LOCUS       sequence-366395-        5361 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Xenopus laevis foxd4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Neilson KM, Klein SL, Mhaske P, Mood K, Daar IO, Moody SA
  TITLE     Specific domains of FoxD4/5 activate and repress neural 
            transcription factor genes to control the progression of immature 
            neural ectoderm to differentiating neural plate.
  JOURNAL   Dev Biol. 2012 May 15;365(2):363-75. doi: 
            10.1016/j.ydbio.2012.03.004. Epub 2012 Mar 10.
  PUBMED    22425621
REFERENCE   2  (bases 1 to 5361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2012.03.004"; journalName: "Dev Biol"; date: 
            "2012-05-15- 15"; volume: "365"; issue: "2"; pages: "363-75"
COMMENT     Sequence Label: pCS2-foxd4l1.1-deltaAB-myc
FEATURES             Location/Qualifiers
     source          1..5361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             103..132
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             142..171
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             181..210
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             220..249
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             259..288
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             322..351
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    1413..1547
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1437..1456)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1491..1510
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1660..1680)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1660..1678)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1699..1715)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1699..1715)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1712..1734)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1723..1739
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1747..1753,1754..1771,1772..1777))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1792..1813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1930..1947)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2101..2689)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2181..2200)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2860..3651,3652..3720))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3483..3502
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3721..3825)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3851..4306)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3988..4009)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4200..4219
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4377..5361
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga tttaaagcta tggagcaaaa gctcatttct
      121 gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg
      181 gagcaaaagc tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa
      241 gaggacttga atgaaatgga gcaaaagctc atttctgaag aggacttgaa tgaaatggag
      301 agcttgggcg acctcaccat ggagcaaaag ctcatttctg aagaggactt gaattcaagg
      361 cctatgagct ttagccagga gtctggtgcc catcatcacc cccaggatta tgcaggactt
      421 tctccctgca gcctaaagtc acacttttac ctacagccca cacattcagt gatgggggac
      481 agtgagatgc tgagtccttc caagctcagc tgcactgaaa gtgagagtga ttcctctgga
      541 gagagtgaag gaggtacttc taaagactcc tcaaccaccc caactggtag caaagccaaa
      601 aggactctgg taaagcctcc ttactcttac attgctctaa tcaccatggc tatcctgcag
      661 agtccccaca agaaactgac tctcagtggc atctgtgatt ttatcagcag caagttccct
      721 tactacaagg acaagttccc tgcttggcag aattccatca ggcacaacct gtcccttaat
      781 gactgcttta ttaaaatacc acgggaacca ggaaatccgg gaaaaggcaa ttattggaca
      841 ctggaccctg cttctgagga catgtttgat aacggaagct tccttagaag gaggaaaagg
      901 tttaagaggc accaacagga gttctttaag gatggactca tgatgtacaa ctctctgcct
      961 tactacagac cctacagtgc tatccaacct cagccagtgc tccagcagac ttcactgaca
     1021 tgcatggcaa taccagagac cttgcccatg tcaactcacc ttgcacccta cccagacata
     1081 aagaggaagg tttcttaccc tgcccagggg gtacacaggg gttttaaagc ccaggatgct
     1141 gataaccatc ccaataattc acagagcaaa tgttcattca gtattgagaa catcatgagg
     1201 aaacccaagg agccagagcc aaatattcaa agcttcaact cacactggaa ctataatcat
     1261 gtattccaga ggccaagttc ctgccttctc ccagctgtgc ttaacttatc cactggccct
     1321 ctgctagcca atactcaggg tgccaggcaa tacaacctca tacaattccc tgggtgttac
     1381 tgatctagaa ctatagtgag tcgtattacg tagatccaga catgataaga tacattgatg
     1441 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     1501 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     1561 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa ttcgcggccg
     1621 cggcgccaat gcattgggcc cggtacccag cttttgttcc ctttagtgag ggttaattgc
     1681 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     1741 tccacacaac atacgagccg ggagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     1801 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     1861 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     1921 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1981 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2041 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2101 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2161 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2221 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2281 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     2341 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     2401 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2461 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2521 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     2581 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2641 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2701 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2761 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2821 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2881 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2941 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3001 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3061 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3121 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3181 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3241 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3301 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3361 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3421 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3481 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3541 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3601 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3661 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3721 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3781 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     3841 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     3901 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     3961 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     4021 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     4081 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     4141 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     4201 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     4261 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
     4321 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagtcg
     4381 accgccaatt caatatggcg tatatggact catgccaatt caatatggtg gatctggacc
     4441 tgtgccaatt caatatggcg tatatggact cgtgccaatt caatatggtg gatctggacc
     4501 ccagccaatt caatatggcg gacttggcac catgccaatt caatatggcg gacctggcac
     4561 tgtgccaact ggggaggggt ctacttggca cggtgccaag tttgaggagg ggtcttggcc
     4621 ctgtgccaag tccgccatat tgaattggca tggtgccaat aatggcggcc atattggcta
     4681 tatgccagga tcaatatata ggcaatatcc aatatggccc tatgccaata tggctattgg
     4741 ccaggttcaa tactatgtat tggccctatg ccatatagta ttccatatat gggttttcct
     4801 attgacgtag atagcccctc ccaatgggcg gtcccatata ccatatatgg ggcttcctaa
     4861 taccgcccat agccactccc ccattgacgt caatggtctc tatatatggt ctttcctatt
     4921 gacgtcatat gggcggtcct attgacgtat atggcgcctc ccccattgac gtcaattacg
     4981 gtaaatggcc cgcctggctc aatgcccatt gacgtcaata ggaccaccca ccattgacgt
     5041 caatgggatg gctcattgcc cattcatatc cgttctcacg ccccctattg acgtcaatga
     5101 cggtaaatgg cccacttggc agtacatcaa tatctattaa tagtaacttg gcaagtacat
     5161 tactattgga agtacgccag ggtacattgg cagtactccc attgacgtca atggcggtaa
     5221 atggcccgcg atggctgcca agtacatccc cattgacgtc aatggggagg ggcaatgacg
     5281 caaatgggcg ttccattgac gtaaatgggc ggtaggcgtg cctaatggga ggtctatata
     5341 agcaatgctc gtttagggaa c
//
