LOCUS       sequence-36643-e         550 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for NTAP (N-terminal affinity purification), 
            frame B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR1A-NTAP-B (w323-1)
FEATURES             Location/Qualifiers
     source          1..550
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    86..110
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             142..219
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             238..351
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
ORIGIN
        1 ctnncngggc ccaatangat tttattttga ctggatagtg acctgttcgt tgcaacaaat
       61 tgataagcaa tgctttttta taatgccaac tttgtacaaa aaagcaggct ttaaaggaac
      121 ccggtggcgg ccgccaccat gaagcgacga tggaaaaaga atttcatagc cgtctcagca
      181 gccaaccgct ttaagaaaat ctcatcctcc ggggcacttg gaagcggtag cggtaccatg
      241 gacgagaaga ccaccggctg gcggggcggc cacgtggtgg agggcctggc cggcgagctg
      301 gagcagctgc gggccaggct ggagcaccac cctcagggcc agcgggagcc ctccggcggc
      361 tgcaagctgg gctgcccggg cggatccccc gggctgcagg aattcgatat caagcttatc
      421 gataccgtng acntcntaga tatctagacc canctttctt gnacnangtt ggnattntaa
      481 gaangcgttg cntatcaatt tgttgcgncg aatgaggtca cnatnagttc anaaataaaa
      541 tcntctattt
//
