LOCUS       sequence-366439-        8846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-dependent expression of mouse cGAS gene in mammalian 
            cells by retroviral transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8846)
  AUTHORS   Mosallanejad K, Kennedy SN, Bahleda KM, Slavik KM, Zhou W, Govande 
            AA, Hancks DC, Kranzusch PJ, Kagan JC
  TITLE     Species-specific self-DNA detection mechanisms by mammalian cyclic 
            GMP-AMP synthases.
  JOURNAL   Sci Immunol. 2023 Jan 20;8(79):eabp9765. doi: 
            10.1126/sciimmunol.abp9765. Epub 2023 Jan 20.
  PUBMED    36662885
REFERENCE   2  (bases 1 to 8846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2023-01-20"; volume: "8(79):eabp9765. doi"; pages:
            " 10.1126/sciimmunol.abp9765. Epub 2023 Jan 20"
COMMENT     Sequence Label: pTRE3G-mouse cGAS-GFP
FEATURES             Location/Qualifiers
     source          1..8846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        318..521
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             522..697
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    760..959
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1160..1576
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1506..1528
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1544..1560
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        1616..1965
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    1624..1642
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1660..1678
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1696..1714
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1732..1750
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1768..1786
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1804..1822
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1840..1858
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1904..1928
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1972..1981
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3514..3516,3517..4230)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(3514..3516,3517..4227)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3556..3577)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3817..3836)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4164..4185
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        4261..4760
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4296..4319)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4693..4712
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4781..5380
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4781..4800)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5277..5297
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5561..5986
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(6089..6111)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6247..6267)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6252..6581
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6432..6567
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6603..6622
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6703..6720)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6874..7462)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6954..6973)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7633..8424,8425..8493))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8256..8275
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8494..8598)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8666..8684
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(8784..8846,1..317)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
       61 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      121 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      181 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      241 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      301 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      361 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      421 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      481 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcaataaaag agcccacaac
      541 ccctcactcg gcgcgccagt cttccgatag actgcgtcgc ccgggtaccc gtattcccaa
      601 taaagcctct tgctgtttgc atccgaatcg tggtctcgct gttccttggg agggtctcct
      661 ctgagtgatt gactacccac gacgggggtc tttcatttgg gggctcgtcc gggatttgga
      721 gacccctgcc cagggaccac cgacccacca ccgggaggta agctggccag caacttatct
      781 gtgtctgtcc gattgtctag tgtctatgtt tgatgttatg cgcctgcgtc tgtactagtt
      841 agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttct gaacacccgg
      901 ccgcaaccct gggagacgtc ccagggactt tgggggccgt ttttgtggcc cgacctgagg
      961 aagggagtcg atgtggaatc cgaccccgtc aggatatgtg gttctggtag gagacgagaa
     1021 cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg gtttggaacc gaagccgcgc
     1081 gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt ctctgtctga ctgtgtttct
     1141 gtatttgtct gaaaattagg gccagactgt taccactccc ttaagtttga ccttaggtca
     1201 ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga agagacgttg
     1261 ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc gagacggcac
     1321 ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg
     1381 acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc
     1441 ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc
     1501 tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac
     1561 tccttctcta ggcgccggaa ttgaagatct cgatgaggcc ctttcgtctt cactcgagtt
     1621 tactccctat cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac
     1681 gtatgcagac tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca
     1741 gtgatagaga acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt
     1801 tactccctat cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac
     1861 gtatgtcgag gtaggcgtgt acggtgggcg cctataaaag cagagctcgt ttagtgaacc
     1921 gtcagatcgc ctggagcaat tccacaacac ttttgtctta tacttggatc cgccaccatg
     1981 gaagatccgc gtagaaggac gacggcgcca cgcgctaaga agccgtccgc gaagcgcgcc
     2041 ccgacgcagc ccagcaggac cagggcccac gcggaaagct gcggcccgca aaggggggct
     2101 cgatcgcggc gggcggagcg tgacggggac accacggaga agccacgtgc cccagggccc
     2161 cgagtgcatc cagcaagggc cactgagctc accaaagatg cacagccctc ggccatggac
     2221 gcggcaggag ccaccgcgcg gcctgccgtc cgggtgcccc agcagcaggc catcctggat
     2281 ccggagctgc ccgccgtacg ggagccccag ccgcccgcgg atcccgaggc gcggaaagtc
     2341 gtaaggggac ctagccacag aaggggcgcg cgctccaccg ggcagcccag agcgccgcga
     2401 gggtccggga aggaaccgga caagctaaag aaggtgctgg acaaattgag attgaaacgc
     2461 aaagatatct cggaggcggc cgagacggtg aataaagttg tggaacgcct gctgcgcaga
     2521 atgcagaaac gggagtcgga gttcaaaggt gtggagcagc tgaacactgg cagctactat
     2581 gaacatgtga agatttctgc tcctaatgaa tttgatgtta tgtttaaact ggaagtcccc
     2641 aggattgagc tacaagaata ttatgaaaca ggtgctttct atcttgtgaa attcaaaaga
     2701 attccacgag gaaatccgct gagtcatttc ttagaagggg aagtattatc agctaccaag
     2761 atgctgtcaa agtttaggaa aatcattaaa gaagaagtta aagaaatcaa agatatagat
     2821 gtcagtgtgg agaaggaaaa accaggaagc cctgctgtaa cacttcttat caggaaccct
     2881 gaagaaatct ctgtggatat aattctggct ttggagtcaa aaggcagctg gcctattagt
     2941 accaaagaag gactacctat tcaaggctgg ctgggcacaa aagtgaggac caatctaaga
     3001 cgagagccgt tttatctcgt acccaagaat gcaaaggatg gaaatagttt tcaaggagag
     3061 acctggcgcc tctctttctc tcacactgaa aagtacattt tgaataatca cgggatagag
     3121 aaaacatgct gtgaatcttc cggagcaaaa tgctgcagaa aagaatgttt aaaattaatg
     3181 aaataccttt tggaacagtt gaaaaaagag tttcaagagc tggatgcatt ctgttcctac
     3241 catgtgaaaa ctgccatctt tcacatgtgg acccaggacc cgcaggacag tcagtgggac
     3301 cccaggaacc tcagctcctg cttcgataag ttgttagcat tctttcttga gtgcctcagg
     3361 acagagaaac tggatcatta ttttattcca aagttcaatc tattctctca agaactaatt
     3421 gaccgaaaaa gtaaagaatt tctatcgaag aaaattgaat atgaaagaaa taatgggttt
     3481 ccaatttttg acaagcttgg tggcggaggt agtgtgagca agggcgagga gctgttcacc
     3541 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     3601 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     3661 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     3721 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     3781 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     3841 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     3901 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     3961 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     4021 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     4081 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     4141 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     4201 actctcggca tggacgagct gtacaagtaa gcggccgcgc cggcgggccc gaattctacc
     4261 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     4321 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
     4381 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
     4441 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
     4501 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
     4561 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
     4621 gtgggtccgg gggcgggctc aggggcnngc tcaggggcgg ggcgggcgcc cgaaggtcct
     4681 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     4741 cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac
     4801 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     4861 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     4921 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     4981 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     5041 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     5101 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     5161 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     5221 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     5281 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     5341 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca
     5401 gcgcccgacc gaaaggagcg cacgacccca tgcatcggcg tctcgagata tcagtggtcc
     5461 aggctctagt tttgactcaa caatatcacc agctgaagcc tatagagtac gagccataga
     5521 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
     5581 ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga
     5641 gaatagagaa gttcagatca aggtcaggaa cagatggaac agggtcgacc ctagagaacc
     5701 atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa
     5761 ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga
     5821 gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg
     5881 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag
     5941 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg ggctcgtccg
     6001 ggatcgggag acccctgccc agggaccacc gacccaccac cgggaggtaa gctggctgcc
     6061 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     6121 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     6181 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtagatcc
     6241 ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     6301 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     6361 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     6421 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     6481 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     6541 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttact ggcttaacta
     6601 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     6661 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
     6721 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6781 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6841 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6901 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6961 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7021 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     7081 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     7141 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7201 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     7261 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     7321 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7381 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7441 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7501 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7561 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7621 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7681 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7741 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7801 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7861 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7921 tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg
     7981 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     8041 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     8101 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     8161 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     8221 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac
     8281 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     8341 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     8401 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8461 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8521 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     8581 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     8641 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga
     8701 attagcttgg ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc
     8761 atgtccaaca ttaccgccat gttgacattg attattgact agttattaat agtaatcaat
     8821 tacggggtca ttagttcata gcccat
//
