LOCUS       sequence-366443-        9074 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human cGAS gene in mammalian cells by retroviral 
            transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9074)
  AUTHORS   Mosallanejad K, Kennedy SN, Bahleda KM, Slavik KM, Zhou W, Govande 
            AA, Hancks DC, Kranzusch PJ, Kagan JC
  TITLE     Species-specific self-DNA detection mechanisms by mammalian cyclic 
            GMP-AMP synthases.
  JOURNAL   Sci Immunol. 2023 Jan 20;8(79):eabp9765. doi: 
            10.1126/sciimmunol.abp9765. Epub 2023 Jan 20.
  PUBMED    36662885
REFERENCE   2  (bases 1 to 9074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2023-01-20"; volume: "8(79):eabp9765. doi"; pages:
            " 10.1126/sciimmunol.abp9765. Epub 2023 Jan 20"
COMMENT     Sequence Label: pLenti-CMV-human cGASdeltaN-OMM
FEATURES             Location/Qualifiers
     source          1..9074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(92..160,161..952)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(310..329)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1123..1711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1612..1631
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1865..1882
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1999..2020
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2035..2040,2041..2058,2059..2065)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2073..2089
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2078..2100
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2097..2113
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2097..2113
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2132..2152
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2134..2152
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2180..2406
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2407..2587
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2634..2759
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3252..3485
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3670..3714
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3981..4097
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4120..4423
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4424..4627
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4578..4598
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4624..4648
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    5925..6513
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5978..5998)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6396..6407)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6527..7014
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6562..6585)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6947..6966
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7035..7634
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7035..7054)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7531..7551
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7772..8005
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8077..8198
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8114..8133)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8168..8187
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8238..8373
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8393..8412)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8394..8412)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8422..8439)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8422..8438)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8431..8453)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8580..9035
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8667..8686)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8877..8898
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        join(9061..9074,1..91)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
       61 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      121 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      181 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      241 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      301 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
      361 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      421 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
      481 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
      541 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
      601 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
      661 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
      721 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
      781 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
      841 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
      901 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
      961 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     1021 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     1081 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1141 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1201 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1261 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1321 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1381 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1441 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     1501 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     1561 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     1621 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     1681 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     1741 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     1801 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     1861 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     1921 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     1981 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     2041 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     2101 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     2161 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
     2221 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
     2281 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
     2341 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
     2401 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     2461 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     2521 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     2581 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     2641 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     2701 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     2761 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     2821 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     2881 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     2941 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     3001 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     3061 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     3121 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     3181 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     3241 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     3301 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     3361 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     3421 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     3481 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     3541 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     3601 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     3661 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     3721 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     3781 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     3841 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     3901 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     3961 tctcgacggt atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     4021 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     4081 acaaaattca aaattttatc gataagcttg ggagttccgc gttacataac ttacggtaaa
     4141 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     4201 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     4261 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     4321 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     4381 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     4441 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     4501 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     4561 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     4621 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     4681 ccatagaaga caccgactct agagccacca tgcctggggc ctcgaagctc cgggcggttt
     4741 tggagaagtt gaagctcagc cgcgatgata tctccacggc ggcggggatg gtgaaagggg
     4801 ttgtggacca cctgctgctc agactgaagt gcgactccgc gttcagaggc gtcgggctgc
     4861 tgaacaccgg gagctactat gagcacgtga agatttctgc acctaatgaa tttgatgtca
     4921 tgtttaaact ggaagtcccc agaattcaac tagaagaata ttccaacact cgtgcatatt
     4981 actttgtgaa atttaaaaga aatccgaaag aaaatcctct gagtcagttt ttagaaggtg
     5041 aaatattatc agcttctaag atgctgtcaa agtttaggaa aatcattaag gaagaaatta
     5101 acgacattaa agatacagat gtcatcatga agaggaaaag aggagggagc cctgctgtaa
     5161 cacttcttat tagtgaaaaa atatctgtgg atataaccct ggctttggaa tcaaaaagta
     5221 gctggcctgc tagcacccaa gaaggcctgc gcattcaaaa ctggctttca gcaaaagtta
     5281 ggaagcaact acgactaaag ccattttacc ttgtacccaa gcatgcaaag gaaggaaatg
     5341 gtttccaaga agaaacatgg cggctatcct tctctcacat cgaaaaggaa attttgaaca
     5401 atcatggaaa atctaaaacg tgctgtgaaa acaaagaaga gaaatgttgc aggaaagatt
     5461 gtttaaaact aatgaaatac cttttagaac agctgaaaga aaggtttaaa gacaaaaaac
     5521 atctggataa attctcttct tatcatgtga aaactgcctt ctttcacgta tgtacccaga
     5581 accctcaaga cagtcagtgg gaccgcaaag acctgggcct ctgctttgat aactgcgtga
     5641 catactttct tcagtgcctc aggacagaaa aacttgagaa ttattttatt cctgaattca
     5701 atctattctc tagcaactta attgacaaaa gaagtaaaga atttctgaca aagcaaattg
     5761 aatatgaaag aaacaatgag tttccagttt ttgatgaatt tgagagggag gtgccatgcc
     5821 acaggccctc acctggggct ctgtggctcc aggtggctgt gacaggggtg ctggtagtca
     5881 cactcctggt ggtgctgtac cggcggcgtc tgcactaggt cgacaatcaa cctctggatt
     5941 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     6001 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     6061 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     6121 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     6181 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     6241 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     6301 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     6361 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     6421 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     6481 cgagtcggat ctccctttgg gccgcctccc cgcctggaat tctaccgggt aggggaggcg
     6541 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac
     6601 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg
     6661 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc
     6721 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc
     6781 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa
     6841 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc
     6901 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     6961 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
     7021 agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     7081 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     7141 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     7201 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     7261 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     7321 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     7381 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     7441 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     7501 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     7561 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     7621 agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac
     7681 cccatgcatc tcgagggccc ggtaccttta agaccaatga cttacaaggc agctgtagat
     7741 cttagccact ttttaaaaga aaagggggga ctggaagggc tagctcactc ccaacgaaga
     7801 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     7861 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7921 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7981 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     8041 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     8101 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8161 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     8221 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     8281 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     8341 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     8401 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     8461 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     8521 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     8581 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     8641 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     8701 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     8761 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     8821 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     8881 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     8941 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     9001 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     9061 cgcggaaccc ctat
//
