LOCUS       sequence-366446-        9077 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of orangutan cGAS gene in mammalian cells by retroviral 
            transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9077)
  AUTHORS   Mosallanejad K, Kennedy SN, Bahleda KM, Slavik KM, Zhou W, Govande 
            AA, Hancks DC, Kranzusch PJ, Kagan JC
  TITLE     Species-specific self-DNA detection mechanisms by mammalian cyclic 
            GMP-AMP synthases.
  JOURNAL   Sci Immunol. 2023 Jan 20;8(79):eabp9765. doi: 
            10.1126/sciimmunol.abp9765. Epub 2023 Jan 20.
  PUBMED    36662885
REFERENCE   2  (bases 1 to 9077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2023-01-20"; volume: "8(79):eabp9765. doi"; pages:
            " 10.1126/sciimmunol.abp9765. Epub 2023 Jan 20"
COMMENT     Sequence Label: pLenti-CMV-orangutan cGASdeltaN-OMM
FEATURES             Location/Qualifiers
     source          1..9077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(105..173,174..965)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(323..342)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1136..1724
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1625..1644
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1878..1895
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2012..2033
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2048..2053,2054..2071,2072..2078)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2086..2102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2091..2113
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2110..2126
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2110..2126
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2145..2165
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2147..2165
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2193..2419
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2420..2600
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2647..2772
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3265..3498
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3683..3727
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3994..4110
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4133..4436
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4437..4640
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4591..4611
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4637..4661
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    5941..6529
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5994..6014)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6412..6423)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6543..7030
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6578..6601)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6963..6982
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7051..7650
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7051..7070)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7547..7567
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7788..8021
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8093..8214
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8130..8149)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8184..8203
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8254..8389
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8409..8428)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8410..8428)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8438..8455)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8438..8454)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8447..8469)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8596..9051
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8683..8702)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8893..8914
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        join(9077,1..104)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
       61 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
      121 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
      181 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
      241 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
      301 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
      361 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
      421 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
      481 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
      541 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
      601 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
      661 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
      721 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
      781 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
      841 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
      901 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
      961 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     1021 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     1081 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1141 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1201 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1261 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     1321 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1381 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     1441 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1501 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     1561 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     1621 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     1681 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     1741 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     1801 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     1861 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     1921 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     1981 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     2041 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     2101 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     2161 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     2221 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     2281 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     2341 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     2401 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     2461 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     2521 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     2581 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     2641 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     2701 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     2761 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     2821 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     2881 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     2941 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     3001 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     3061 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     3121 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     3181 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     3241 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     3301 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     3361 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     3421 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     3481 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     3541 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     3601 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     3661 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     3721 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     3781 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     3841 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     3901 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     3961 attagtgaac ggatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg
     4021 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     4081 acaaaaacaa attacaaaat tcaaaatttt atcgataagc ttgggagttc cgcgttacat
     4141 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     4201 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     4261 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     4321 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     4381 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     4441 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     4501 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     4561 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     4621 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
     4681 gctgttttga cctccataga agacaccgac tctagagcca ccatgcctgg ggcctcgaag
     4741 ctccgggcgg ttttggagaa gttgaagctc agccgcctgg aaatctccaa ggcggcggag
     4801 gtggtgaaca gggttgtgga ccacctgctg cgcagactgc agaagtacaa ctccgagttc
     4861 agaggcgtcg ggctgctgcg caccgggagc tactatgagc acgtgaagat ttctgcacct
     4921 aatgaatttg atgtcatgtt taaactggaa gtccccagaa ttcaactaga agaatattcc
     4981 aacactggtg catattactt tgtgaaattt aaaagaaatc cgaaagaaaa tcctctgagt
     5041 cagtttttag aaggtgaaat attatcagct tctaagatgc tgtcaaagtt taggaaaatc
     5101 attaaggaag aaattaacaa cattaaagat acagatgtca tcatgaagag gaaaagagga
     5161 gggagccctg ctgtaacact tcttattagt gaagaaatat ctgtggatat aaccctggct
     5221 ttggaatcaa aaagtagctg gcctgctagc acccaagaag gcctgcccat taaaaactgg
     5281 ctttcagcaa aagttaggaa gcaactaaga ctaaagccat tttaccttgt acccaagcat
     5341 gcaaaggaag gaaatggttt ccaagaagaa acatggcggc tatccttctc tcacatcgaa
     5401 aaggaaattt tgaacaatca tggaaaatct aaaacgtgct gtgaagacaa agaagtgaaa
     5461 tgttgcagga aagattgttt aaaactaatg aaataccttt tagaacagct gaaagaaagg
     5521 tttaaagaca aaaaacatct ggataaattc tcttcttatc atgtgaaaac tgccttcttt
     5581 cacgtatgta cccagaaccc tcaagacagt cagtgggacc ccaaagacct gggcctctgc
     5641 tttgataact gcgtgacata ctttcttcag tgcctcagga cagaacaact tgggaattat
     5701 tttattcctg gattcaatct attctctagc aacttaattg acaaaagaag taaagaattt
     5761 ctgacaaagc aaattgaata tgaaagaaac aatgagtttc cagtttttga tgaatttgag
     5821 agggaggtgc catgccacag gccctcacct ggggctctgt ggctccaggt ggctgtgaca
     5881 ggggtgctgg tagtcacact cctggtggtg ctgtaccggc ggcgtctgca ctaggtcgac
     5941 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     6001 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     6061 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     6121 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     6181 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     6241 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     6301 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     6361 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     6421 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     6481 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcta
     6541 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct
     6601 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag
     6661 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt
     6721 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc
     6781 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct
     6841 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag
     6901 gggtgggtcc gggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
     6961 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
     7021 gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc
     7081 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     7141 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     7201 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     7261 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     7321 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     7381 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     7441 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     7501 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     7561 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     7621 tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc
     7681 gaaaggagcg cacgacccca tgcatctcga gggcccggta cctttaagac caatgactta
     7741 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctagc
     7801 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     7861 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7921 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7981 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     8041 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     8101 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     8161 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     8221 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8281 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     8341 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     8401 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     8461 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     8521 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     8581 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     8641 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     8701 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     8761 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     8821 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     8881 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     8941 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     9001 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     9061 ttttcgggga aatgtgc
//
