LOCUS       sequence-366449-        9102 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of orangutan cGAS gene in mammalian cells by retroviral 
            transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9102)
  AUTHORS   Mosallanejad K, Kennedy SN, Bahleda KM, Slavik KM, Zhou W, Govande 
            AA, Hancks DC, Kranzusch PJ, Kagan JC
  TITLE     Species-specific self-DNA detection mechanisms by mammalian cyclic 
            GMP-AMP synthases.
  JOURNAL   Sci Immunol. 2023 Jan 20;8(79):eabp9765. doi: 
            10.1126/sciimmunol.abp9765. Epub 2023 Jan 20.
  PUBMED    36662885
REFERENCE   2  (bases 1 to 9102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2023-01-20"; volume: "8(79):eabp9765. doi"; pages:
            " 10.1126/sciimmunol.abp9765. Epub 2023 Jan 20"
COMMENT     Sequence Label: pLenti-CMV-FLAG-orangutan cGASdeltaN-OMM
FEATURES             Location/Qualifiers
     source          1..9102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        14..118
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(119..187,188..979)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(337..356)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1150..1738
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1639..1658
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1892..1909
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2026..2047
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2062..2067,2068..2085,2086..2092)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2100..2116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2105..2127
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2124..2140
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2124..2140
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2159..2179
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2161..2179
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2207..2433
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2434..2614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2661..2786
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3279..3512
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3697..3741
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4008..4124
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4147..4450
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4451..4654
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4605..4625
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4651..4675
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4732..4741
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4741..4764
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    5980..6568
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6033..6053)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6451..6462)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6582..7069
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6617..6640)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7002..7021
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7090..7689
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7090..7109)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7586..7606
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7827..8060
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8132..8253
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8169..8188)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8223..8242
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8293..8428
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8448..8467)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8449..8467)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8477..8494)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8477..8493)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8486..8508)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8635..9090
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8722..8741)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8932..8953
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
       61 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      121 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      181 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      241 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      301 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      361 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      421 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      481 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
      541 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
      601 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
      661 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
      721 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
      781 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
      841 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
      901 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
      961 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1021 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1081 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1141 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1201 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1261 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1321 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1381 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1441 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1501 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1561 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1621 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1681 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     1741 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     1801 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     1861 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     1921 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     1981 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     2041 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     2101 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     2161 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
     2221 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
     2281 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
     2341 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
     2401 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
     2461 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     2521 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     2581 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     2641 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     2701 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     2761 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     2821 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     2881 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     2941 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     3001 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     3061 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     3121 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     3181 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     3241 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     3301 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     3361 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     3421 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     3481 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     3541 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     3601 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     3661 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     3721 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     3781 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     3841 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     3901 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     3961 gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa
     4021 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     4081 caaactaaag aattacaaaa acaaattaca aaattcaaaa ttttatcgat aagcttggga
     4141 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     4201 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     4261 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     4321 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     4381 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     4441 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     4501 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     4561 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     4621 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     4681 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgactctaga cgccaccatg
     4741 gattacaagg atgacgacga taagcctggg gcctcgaagc tccgggcggt tttggagaag
     4801 ttgaagctca gccgcctgga aatctccaag gcggcggagg tggtgaacag ggttgtggac
     4861 cacctgctgc gcagactgca gaagtacaac tccgagttca gaggcgtcgg gctgctgcgc
     4921 accgggagct actatgagca cgtgaagatt tctgcaccta atgaatttga tgtcatgttt
     4981 aaactggaag tccccagaat tcaactagaa gaatattcca acactggtgc atattacttt
     5041 gtgaaattta aaagaaatcc gaaagaaaat cctctgagtc agtttttaga aggtgaaata
     5101 ttatcagctt ctaagatgct gtcaaagttt aggaaaatca ttaaggaaga aattaacaac
     5161 attaaagata cagatgtcat catgaagagg aaaagaggag ggagccctgc tgtaacactt
     5221 cttattagtg aagaaatatc tgtggatata accctggctt tggaatcaaa aagtagctgg
     5281 cctgctagca cccaagaagg cctgcccatt aaaaactggc tttcagcaaa agttaggaag
     5341 caactaagac taaagccatt ttaccttgta cccaagcatg caaaggaagg aaatggtttc
     5401 caagaagaaa catggcggct atccttctct cacatcgaaa aggaaatttt gaacaatcat
     5461 ggaaaatcta aaacgtgctg tgaagacaaa gaagtgaaat gttgcaggaa agattgttta
     5521 aaactaatga aatacctttt agaacagctg aaagaaaggt ttaaagacaa aaaacatctg
     5581 gataaattct cttcttatca tgtgaaaact gccttctttc acgtatgtac ccagaaccct
     5641 caagacagtc agtgggaccc caaagacctg ggcctctgct ttgataactg cgtgacatac
     5701 tttcttcagt gcctcaggac agaacaactt gggaattatt ttattcctgg attcaatcta
     5761 ttctctagca acttaattga caaaagaagt aaagaatttc tgacaaagca aattgaatat
     5821 gaaagaaaca atgagtttcc agtttttgat gaatttgaga gggaggtgcc atgccacagg
     5881 ccctcacctg gggctctgtg gctccaggtg gctgtgacag gggtgctggt agtcacactc
     5941 ctggtggtgc tgtaccggcg gcgtctgcac taggtcgaca atcaacctct ggattacaaa
     6001 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     6061 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     6121 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     6181 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     6241 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     6301 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     6361 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     6421 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     6481 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     6541 cggatctccc tttgggccgc ctccccgcct ggaattctac cgggtagggg aggcgctttt
     6601 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag
     6661 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
     6721 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
     6781 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
     6841 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
     6901 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct
     6961 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa
     7021 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc
     7081 aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     7141 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     7201 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     7261 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     7321 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     7381 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     7441 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     7501 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     7561 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     7621 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     7681 ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat
     7741 gcatctcgag ggcccggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     7801 ccacttttta aaagaaaagg ggggactgga agggctagct cactcccaac gaagacaaga
     7861 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7921 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7981 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     8041 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     8101 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     8161 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8221 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     8281 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     8341 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     8401 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     8461 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     8521 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     8581 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     8641 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     8701 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     8761 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     8821 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     8881 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     8941 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     9001 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     9061 attttaacaa aatattaacg cttacaattt aggtggcact tt
//
