LOCUS       sequence-36645-e         618 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for NTAP (N-terminal affinity purification), 
            frame A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR1A-NTAP-A (w322-1)
FEATURES             Location/Qualifiers
     source          1..618
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    8..107
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             139..216
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             235..348
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
ORIGIN
        1 ctacngggcc aatatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
       61 taagcaatgc ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaacccg
      121 gtggcggccg ccaccatgaa gcgacgatgg aaaaagaatt tcatagccgt ctcagcagcc
      181 aaccgcttta agaaaatctc atcctccggg gcacttggaa gcggtagcgg taccatggac
      241 gagaagacca ccggctggcg gggcggccac gtggtggagg gcctggccgg cgagctggag
      301 cagctgcggg ccaggctgga gcaccaccct cagggccagc gggagccctc cggcggctgc
      361 aagctgggcg cccgggngga tcccccgggc tgtaggaatt cnatntcaag cttatnnatn
      421 ctgtcgaccn ngagatatct tnncncagcn ttcangntcg anggcngnat ttttacnnag
      481 nattgcagtn tcgctttntt gcttcgtnac aggtgcacta ttcagtcaan atntatntca
      541 tttantagcn attcngntcn canntncann ncnnacngtc ttncctatta acngatntta
      601 tncntgtcct naggatca
//
