LOCUS       sequence-366452-        9587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  broad-host range plasmid to constitutively express fluorescent 
            protein genes in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9587)
  AUTHORS   Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, 
            Dobson RCJ, Remus DM, Remus-Emsermann MNP
  TITLE     Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal 
            Insertion Toolbox for Fluorescent Protein Expression in Bacteria.
  JOURNAL   Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. 
            eCollection 2018.
  PUBMED    30631309
REFERENCE   2  (bases 1 to 9587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2018-12-12"; volume: "9:3052. doi"; pages: " 
            10.3389/fmicb.2018.03052. eCollection 2018"
COMMENT     Sequence Label: pMRE164
FEATURES             Location/Qualifiers
     source          1..9587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1023..1794
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1795..2457
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     3151..3173
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3165..3182
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3166..3182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3192..3211
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3192..3210
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      3253..3339
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3436..3522
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3619..3705
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3802..3888
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3990..5189)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             6322..7032
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     terminator      7049..7143
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             7187..7846
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7253..7272)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      7911..7997
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(8336..9130)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8447..8466)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9057..9076
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaagggcgaa gcccgcccgc
     1381 ctgccccccg agcctcacgg cggcgagtgc gggggttcca agggggcagc gccaccttgg
     1441 gcaaggccga aggccgcgca gtcgatcaac aagccccgga ggggccactt tttgccggag
     1501 ggggagccgc gccgaaggcg tgggggaacc ccgcaggggt gcccttcttt gggcaccaaa
     1561 gaactagata tagggcgaaa tgcgaaagac ttaaaaatca acaacttaaa aaaggggggt
     1621 acgcaacagc tcattgcggc accccccgca atagctcatt gcgtaggtta aagaaaatct
     1681 gtaattgact gccactttta cgcaacgcat aattgttgtc gcgctgccga aaagttgcag
     1741 ctgattgcgc atggtgccgc aaccgtgcgg cacccctacc gcatggagat aagcatggcc
     1801 acgcagtcca gagaaatcgg cattcaagcc aagaacaagc ccggtcactg ggtgcaaacg
     1861 gaacgcaaag cgcatgaggc gtgggccggg cttattgcga ggaaacccac ggcggcaatg
     1921 ctgctgcatc acctcgtggc gcagatgggc caccagaacg ccgtggtggt cagccagaag
     1981 acactttcca agctcatcgg acgttctttg cggacggtcc aatacgcagt caaggacttg
     2041 gtggccgagc gctggatctc cgtcgtgaag ctcaacggcc ccggcaccgt gtcggcctac
     2101 gtggtcaatg accgcgtggc gtggggccag ccccgcgacc agttgcgcct gtcggtgttc
     2161 agtgccgccg tggtggttga tcacgacgac caggacgaat cgctgttggg gcatggcgac
     2221 ctgcgccgca tcccgaccct gtatccgggc gagcagcaac taccgaccgg ccccggcgag
     2281 gagccgccca gccagcccgg cattccgggc atggaaccag acctgccagc cttgaccgaa
     2341 acggaggaat gggaacggcg cgggcagcag cgcctgccga tgcccgatga gccgtgtttt
     2401 ctggacgatg gcgagccgtt ggagccgccg acacgggtca cgctgccgcg ccggtagcac
     2461 ttgggttgcg cagcaacccg taagtgcgct gttccagact atcggctgta gccgcctcgc
     2521 cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc
     2581 gacctgaatg gaagccggcg gcacctcgct aacggattca ccgtttttat caggctctgg
     2641 gaggcagaat aaatgatcat atcgtcaatt attacctcca cggggagagc ctgagcaaac
     2701 tggcctcagg catttgagaa gcacacggtc acactgcttc cggtagtcaa taaaccggta
     2761 aaccagcaat agacataagc ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg
     2821 aatttgcttt cgaatttctg ccattcatcc gcttattatc acttattcag gcgtagcaac
     2881 caggcgttta agggcaccaa taactgcctt aaaaaaatta cgccccgccc tgccactcat
     2941 cgcagtacgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     3001 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
     3061 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     3121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     3181 gtgagcgcgc gtaatacgac tcactatagg gcgaattgga gctggggccg caattcccaa
     3241 ttccccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     3301 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
     3361 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggaat
     3421 taattcccca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     3481 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
     3541 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
     3601 aattaattcc ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     3661 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat
     3721 ttgaacgttg cgaagcaacg gcccggaggg dggcgggsar grmgcccgcc ataacctgcc
     3781 aggaattaat tccccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     3841 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg
     3901 gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact
     3961 gccaggaatt ggggatcgga ttcgagctct cagcgatcgg ctcgttgccc tgcgccgctc
     4021 caaagcccgc gacgcagcgc cggcaggcag agcaagtaga gggcagcgcc tgcaatccat
     4081 gcccacccgt tccacgttgt tatagaagcc gcatagatcg ccgtgaagag gaggggtccg
     4141 acgatcgagg tcaggctggt gagcgccgcc agtgagcctt gcagctgccc ctgacgttcc
     4201 tcatccacct gcctggacaa cattgcttgc agcgccggca ttccgatgcc acccgaagca
     4261 agcaggacca tgatcgggaa cgccatccat ccccgtgtcg cgaaggcaag caggatgtag
     4321 cctgtgccgt cggcaatcat tccgagcatg agtgcccgcc tttcgccgag ccgggcggct
     4381 acagggccgg tgatcattgc ctgggcgagt gaatgcagaa tgccaaatgc ggcaagcgaa
     4441 atgccgatcg tggtcgcgtc ccagtgaaag cgatcctcgc cgaaaatgac ccaaagcgcg
     4501 gccggcacct gtccgacaag ttgcatgatg aagaagaccg ccatcagggc ggcgacgacg
     4561 gtcatgcccc gggcccaccg gaacgaagcg agcgggttga gagcctcccg gcgtaacggc
     4621 cggcgttcgc ctttgtgcga ctccggcaaa aggaaacagc ccgtcaggaa attgaggccg
     4681 ttcaaggctg ccgcggcgaa gaacggagcg tggggggaga aaccgcccat cagcccaccg
     4741 agcacaggtc ccgcgaccat cccgaacccg aaacaggcgc tcatgaagcc gaagtgccgc
     4801 gcgcgctcat cgccatcagt gatatcggca atataagcgc cggctaccgc cccagtcgcc
     4861 ccggtgatgc cggccacgat ccgcccgata tagagaaccc aaaggaaagg cgctgtcgcc
     4921 atgatggcgt agtcgacagt ggcgccggcc agcgagacga gcaagattgg ccgccgcccg
     4981 aaacgatccg acagcgcgcc cagcacaggt gcgcaggcaa attgcaccaa cgcatacagc
     5041 gccagcagaa tgccatagtg ggcggtgacg tcgttcgagt gaaccagatc gcgcaggagg
     5101 cccggcagca ccggcataat caggccgatg ccgacagcgt cgagcgcgac agtgctcaga
     5161 attacgatca ggggtatgtt gggtttcacg tctggcctcc ggaccagcct ccgctggtcc
     5221 gattgaacgc gcggattctt tatcactgat aagttggtgg acatattatg tttatcagtg
     5281 ataaagtgtc aagcatgaca aagttgcagc cgaatacagt gatccgtgcc gccctggacc
     5341 tgttgaacga ggtcggcgta gacggtctga cgacacgcaa actggcggaa cggttggggg
     5401 ttcagcagcc ggcgctttac tggcacttca ggaacaagcg ggcgctgctc gacgcactgg
     5461 ccgaagccat gctggcggag aatcatacgc attcggtgcc gagagccgac gacgactggc
     5521 gctcatttct gatcgggaat gcccgcagct tcaggcaggc gctgctcgcc taccgcgatg
     5581 gcgcgcgcat ccatgccggc acgcgaccgg gcgcaccgca gatggaaacg gccgacgcgc
     5641 agcttcgctt cctctgcgag gcgggttttt cggccgggga cgccgtcaat gcgctgatga
     5701 caatcagcta cttcactgtt ggggccgtgc ttgaggagca ggccggcgac agcgatgccg
     5761 gcgagcgcgg cggcaccgtt gaacaggctc cgctctcgcc gctgttgcgg gccgcgatag
     5821 acgccttcga cgaagccggt ccggacgcag cgttcgagca gggactcgcg gtgattgtcg
     5881 atggattggc gaaaaggagg ctcgttgtca ggaacgttga aggaccgaga aagggtgcta
     5941 agaaaccatt attatcatga agctcgcacg ctgccgcaag cactcagggc gcaagggctg
     6001 ctaaaggaag cggaacacgt agaaagccag tccgcagaaa cggtgctgac cccggatgaa
     6061 tgtcagctac tgggctatct ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc
     6121 ttgcagtggg cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc
     6181 ggaattgcca gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat
     6241 ggctttcttg ccgccaagga tctgatggcg caggggatca agatctgatc aagagacagg
     6301 atactagtgg aggaagaaaa aatggtgagc aagggcgagg agaataacat ggccatcatc
     6361 aaggagttca tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca cgagttcgag
     6421 atcgagggcg agggcgaggg ccgcccctac gagggctttc agaccgctaa gctgaaggtg
     6481 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcattt cacctacggc
     6541 tccaaggcct acgtgaagca ccccgccgac atccccgact acttcaagct gtccttcccc
     6601 gagggcttca agtgggagcg cgtgatgaac tacgaggacg gcggcgtggt gaccgtgacc
     6661 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     6721 ttcccctccg acggccccgt gatgcagaag aagaccatgg gctgggaggc ctcctccgag
     6781 cggatgtacc ccgaggacgg tgccctgaag ggcaagatca agatgaggct gaagctgaag
     6841 gacggcggcc actacacctc cgaggtcaag accacctaca aggccaagaa gcccgtgcag
     6901 ctgcccggcg cctacatcgt cgacatcaag ttggacatca cctcccacaa cgaggactac
     6961 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     7021 ctgtacaagt aataattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
     7081 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
     7141 aatccaagct agcttggcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat
     7201 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt
     7261 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt
     7321 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg
     7381 cctgatgaat gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg
     7441 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct
     7501 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc
     7561 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt
     7621 ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa
     7681 cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat
     7741 gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct
     7801 taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag
     7861 ttattggtgc ccttaaacgc ctggggtaat gactctctag cttgaggcat caaataaaac
     7921 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     7981 tcctgagtag gacaaatccg ccgctaggag cttgcggccg ccagtgtgat ggatatctgc
     8041 ataaaaactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
     8101 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     8161 tgggggtggg cgaagaactc cagcatgaga tccccgcgct ggaggatcat ccagccggcg
     8221 tcccggaaaa cgattccgaa gcccaacctt tcatagaagg cggcggtgga atcgaaatct
     8281 cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga accccagagt cccgctcaga
     8341 agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt
     8401 aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag
     8461 ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag
     8521 aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga
     8581 gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc
     8641 cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg
     8701 ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat
     8761 gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg
     8821 acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga
     8881 caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg
     8941 cctcgtcctg cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc
     9001 gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc
     9061 agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt
     9121 gttcaatcat gcgaaacgat cctcatcctg tctcttgatc agatcttgat cccctgcgcc
     9181 atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc ccaaccttac
     9241 cagagggcgc cccagctggc aattccggtt cgcttgctgt ccataaaacc gcccagtcta
     9301 gctatcgcca tgtaagccca ctgcaagcta cctgctttct ctttgcgctt gcgttttccc
     9361 ttgtccagat agcccagtag ctgacattca tcccaggtgg cacttttcgg ggaaatgtgc
     9421 gcgcccgcgt tcctgctggc gctgggcctg tttctggcgc tggacttccc gctgttccgt
     9481 cagcagcttt tcgcccacgg ccttgatgat cgcggcggcc ttggcctgca tatcccgatt
     9541 caacggcccc agggcgtcca gaacgggctt caggcgctcc cgaaggt
//
