LOCUS       sequence-36646-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent viral expression of ArchT-tdTomato for neuronal 
            inhibition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Han X, Chow BY, Zhou H, Klapoetke NC, Chuong A, Rajimehr R, Yang A, 
            Baratta MV, Winkle J, Desimone R, Boyden ES
  TITLE     A high-light sensitivity optical neural silencer: development and 
            application to optogenetic control of non-human primate cortex.
  JOURNAL   Front Syst Neurosci. 2011;5:18. Epub 2011 Apr 13.
  PUBMED    21811444
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Syst 
            Neurosci."; date: "2011"; volume: "5"; pages: "18. Epub 2011 Apr 13"
COMMENT     Sequence Label: pAAV-FLEX-ArchT-tdTomato
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    56..89
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    160..193
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      210..219
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tccagaggtt gattatcgat aagcttgata tcgaattcga acctcttcga gggacataac
       61 ttcgtataat gtatgctata cgaagttatg ggtcgatggt gagatctgga ctagagggtc
      121 gatggtgatg cttggatcaa aataggaaga atcccaatga taacttcgta taaagtatcc
      181 tatacgaagt tatactagaa ttcggtaccg ccaccatgga ccccatcgct ctgcaggcag
      241 gatacgacct gctgggcgac ggaaggccag agaccctgtg gctgggaatc ggaaccctgc
      301 tgatgctgat cggcaccttc tacttcatcg tgaagggctg gggcgtgacc gacaaggagg
      361 ccagggagta ctacagcatc acaatcctgg tgcccggcat cgccagcgcc gcctacctga
      421 gcatgttctt cggcatcggc ctgaccgagg tgaccgtggc cggcgaggtg ctggacatct
      481 actacgccag atacgccgac tggctgttca ccaccccttt gcttctgctc gacctggccc
      541 tgctggctaa ggtggacagg gtgagcatcg gaaccctggt gggagtggac gccctgatga
      601 tcgtgaccgg cctgatcggc gccctgagcc acaccccact ggctaggtac agctggtggc
      661 tgttcagcac catctgcatg atcgtggtgc tgtacttcct ggctaccagc ctgagggctg
      721 ctgctaagga gaggggacca gaggtggcta gcaccttcaa caccctgacc gccctggtgc
      781 tggtgctgtg gaccgcctac cccatcctgt ggatcatcgg aaccgaggga gctggagtgg
      841 tgggactggg aatcgagacc ctgctgttca tggtgctgga cgtgaccgcc aaagt
//
