LOCUS       sequence-366469-       10598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Sox9 and puromycin resistance gene under control of tetO 
            sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10598)
  AUTHORS   Canals I, Ginisty A, Quist E, Timmerman R, Fritze J, Miskinyte G, 
            Monni E, Hansen MG, Hidalgo I, Bryder D, Bengzon J, Ahlenius H
  TITLE     Rapid and efficient induction of functional astrocytes from human 
            pluripotent stem cells.
  JOURNAL   Nat Methods. 2018 Sep;15(9):693-696. doi: 10.1038/s41592-018-0103-2.
            Epub 2018 Aug 20.
  PUBMED    30127505
REFERENCE   2  (bases 1 to 10598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0103-2"; journalName: "Nat Methods"; date: 
            "2018-09"; volume: "15"; issue: "9"; pages: "693-696"
COMMENT     Sequence Label: tetO.Sox9.Puro
FEATURES             Location/Qualifiers
     source          1..10598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1544..1597
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1604..2203
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1604..1623)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2100..2120
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2242..2830
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2295..2315)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2713..2724)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2833..2849)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2834..2850)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3355..3535
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3561..3578)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3567..3791
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3837..4265
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3924..3943)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4134..4155
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4274..4294)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4279..4608
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4459..4594
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4521..4540
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4656..4703
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4722..5096
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5226..5347
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5263..5282)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5317..5336
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5396..5412)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5396..5412)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5409..5431)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5420..5436
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5444..5450,5451..5468,5469..5474))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5489..5510
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5627..5644)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5798..6386)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5878..5897)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6557..7348,7349..7417))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7180..7199
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7418..7522)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7597..7616)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7788..8167
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8169..8367
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8385..8565
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8612..8737
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9230..9463
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9648..9692
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9990..10107
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(10164..10182,10183..10205,10206..10224,10225..10247,
                     10248..10266,10267..10289,10290..10308,10309..10331,
                     10332..10350,10351..10373,10374..10392,10393..10415,
                     10416..10434)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        10467..10505
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10502..10526
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gaattcgccc ttattcatga atctcctgga ccccttcatg aagatgaccg acgagcagga
       61 gaagggcctg tctggcgccc ccagccccac catgtcggag gactcggctg gttcgccctg
      121 tccctcgggc tccggctcgg acacggagaa cacccggccc caggagaaca ccttccccaa
      181 gggcgagccg gatctgaaga aggagagcga ggaagataag ttccccgtgt gcatccgcga
      241 ggcggtcagc caggtgctga agggctacga ctggacgctg gtgcccatgc ccgtgcgcgt
      301 caacggctcc agcaagaaca agccacacgt caagcgaccc atgaacgcct tcatggtgtg
      361 ggcgcaggct gcgcgcagga agctggcaga ccagtacccg catctgcaca acgcggagct
      421 cagcaagact ctgggcaagc tctggaggct gctgaacgag agcgagaaga gacccttcgt
      481 ggaggaggcg gagcggctgc gcgtgcagca caagaaagac caccccgatt acaagtacca
      541 gccccggcgg aggaagtcgg tgaagaacgg acaagcggag gccgaagagg ccacggaaca
      601 gactcacatc tctcctaatg ctatcttcaa ggcgctgcaa gccgactccc cacattcctc
      661 ctccggcatg agtgaggtgc actccccggg cgagcactct gggcaatctc agggtccgcc
      721 gaccccaccc accactccca aaaccgacgt gcaagctggc aaagttgatc tgaagcgaga
      781 ggggcgccct ctggcagagg ggggcaggca gccccccatc gacttccgcg acgtggacat
      841 cggtgaactg agcagcgacg tcatctccaa cattgagacc ttcgacgtca atgagtttga
      901 ccaatacttg ccacccaacg gccacccagg ggttccggcc acccacggcc aggtcaccta
      961 cactggcagt tacggcatca gcagcaccgc acccacccct gcgaccgcgg gccacgtgtg
     1021 gatgtcgaag cagcaggcgc cgccccctcc tccgcagcag cctccgcagg ccccgcaagc
     1081 cccacaggcg cctccgcagc agcaagcacc cccgcagcag ccgcaggcac cccagcagca
     1141 gcaggcacac acgctcacca cgctgagcag cgagccaggc cagtcccagc gaacgcacat
     1201 caagacggag cagctgagcc ccagccacta cagcgagcag cagcagcact ccccgcaaca
     1261 gatctcctac agccccttca accttcctca ctacagcccc tcctacccgc ccatcacccg
     1321 ctcgcaatac gactacgctg accatcagaa ctccggctcc tactacagtc acgcagccgg
     1381 ccagggctca gggctctact ccaccttcac ttacatgaac cccgcgcagc gccccatgta
     1441 cacccccatc gctgacacct ccggggtccc ttccatcccg cagacccaca gcccgcagca
     1501 ctgggaacaa ccagtctaca cacagctcac cagaccctct agagagggca ggggaagtct
     1561 tctaacatgc ggggacgtgg aggaaaatcc cggcccactc gagatgaccg agtacaagcc
     1621 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     1681 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     1741 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     1801 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     1861 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     1921 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     1981 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     2041 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     2101 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     2161 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgattaatta agctagctaa
     2221 ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
     2281 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     2341 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     2401 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     2461 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     2521 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     2581 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     2641 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     2701 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     2761 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     2821 gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt
     2881 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     2941 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     3001 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     3061 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag
     3121 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta
     3181 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta
     3241 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt
     3301 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct
     3361 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     3421 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     3481 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc
     3541 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     3601 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     3661 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     3721 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     3781 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     3841 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     3901 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     3961 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4021 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     4081 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4141 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     4201 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4261 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     4321 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     4381 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     4441 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4501 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     4561 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     4621 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt
     4681 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg
     4741 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
     4801 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
     4861 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
     4921 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
     4981 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
     5041 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg
     5101 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     5161 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     5221 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5281 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5341 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     5401 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     5461 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     5521 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     5581 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     5641 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5701 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5761 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5821 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5881 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5941 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6001 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6061 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6121 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6181 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     6241 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6301 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6361 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6421 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6481 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6541 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6601 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6661 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     6721 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6781 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     6841 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     6901 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     6961 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7021 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7081 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7141 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7201 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7261 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7321 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7381 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7441 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7501 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     7561 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
     7621 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
     7681 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
     7741 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
     7801 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     7861 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     7921 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     7981 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     8041 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     8101 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     8161 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     8221 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     8281 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     8341 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
     8401 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8461 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8521 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     8581 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     8641 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     8701 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     8761 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     8821 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     8881 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     8941 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     9001 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     9061 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     9121 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     9181 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     9241 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     9301 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     9361 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     9421 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     9481 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     9541 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     9601 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     9661 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     9721 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     9781 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     9841 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     9901 agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc
     9961 tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
    10021 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
    10081 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
    10141 tggttagatc tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
    10201 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
    10261 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
    10321 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
    10381 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
    10441 aaagtcgagc tcggtacccg ggtcgaggta ggcgtgtacg gtgggaggcc tatataagca
    10501 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
    10561 atagaagaca ccgggaccga tccagcctcc gcggcccc
//
