LOCUS       sequence-36648-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Manabe R, Kovalenko M, Webb DJ, Horwitz AR
  TITLE     GIT1 functions in a motile, multi-molecular signaling complex that 
            regulates protrusive activity and cell migration.
  JOURNAL   J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-510.
  PUBMED    11896197
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2002 Apr 1. 115(Pt 7):1497-510."
COMMENT     Sequence Label: pEGFP-GIT1
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     204..225
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      267..276
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(315..518)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(344..364)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        519..822
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca
       61 ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt
      121 ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga
      181 cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc
      241 ggtgaacagc tcctcgccct tgctcaccat ggtggcgacc ggtagcgcta gcggatctga
      301 cggttcacta aaccagctct gcttatatag acctcccacc gtacacgcct accgcccatt
      361 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
      421 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
      481 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
      541 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
      601 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
      661 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
      721 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
      781 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
//
