LOCUS       sequence-366534-        9642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-dependent expression of human cGAS gene in mammalian 
            cells by retroviral transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9642)
  AUTHORS   Mosallanejad K, Kennedy SN, Bahleda KM, Slavik KM, Zhou W, Govande 
            AA, Hancks DC, Kranzusch PJ, Kagan JC
  TITLE     Species-specific self-DNA detection mechanisms by mammalian cyclic 
            GMP-AMP synthases.
  JOURNAL   Sci Immunol. 2023 Jan 20;8(79):eabp9765. doi: 
            10.1126/sciimmunol.abp9765. Epub 2023 Jan 20.
  PUBMED    36662885
REFERENCE   2  (bases 1 to 9642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2023-01-20"; volume: "8(79):eabp9765. doi"; pages:
            " 10.1126/sciimmunol.abp9765. Epub 2023 Jan 20"
COMMENT     Sequence Label: pTetOne-FLAG-human cGASdeltaN-HA
FEATURES             Location/Qualifiers
     source          1..9642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(13..29)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13..29)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(26..48)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    37..53
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(61..67,68..85,86..91))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(244..261)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(415..1003)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(495..514)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1174..1965,1966..2034))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1797..1816
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2035..2139)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2207..2225
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2263..2285)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2385..2404
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2598..2620
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2612..2629
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2613..2629
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2652..2773
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3086..3106)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     promoter        complement(4979..5489)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5042..5060)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        5506..5873
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    5514..5532
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5550..5568
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5586..5604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5622..5640
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5658..5676
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5694..5712
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5730..5748
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      5880..5889
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5889..5912
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7002..7028
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    7212..7346
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7494..7510)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     primer_bind     complement(7848..7864)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(7880..7902)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    8313..8670
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     enhancer        9101..9480
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
       61 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
      121 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      181 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      241 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      301 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      361 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      421 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      481 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      541 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      601 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      661 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      721 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      781 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      841 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      901 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      961 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1021 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     1081 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     1141 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1201 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1261 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1321 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1381 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1441 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1501 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1561 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1621 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1681 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1741 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     1801 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1861 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1921 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1981 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     2041 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     2101 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     2161 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2221 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2281 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2341 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2401 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     2461 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     2521 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     2581 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattaattcg
     2641 acgcgccatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     2701 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     2761 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     2821 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc
     2881 tctagagtcg gtgggcctcg ggggcgggtg cggggtcggc ggggccgccc cgggtggctt
     2941 cggtcggagc catggggtcg tgcgctcctt tcggtcgggc gctgcgggtc gtggggcggg
     3001 cgtcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg
     3061 tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg cgcggaggtc
     3121 tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag cacgacggcg
     3181 ctgcccagac ccttgccctg gtggtcgggc gagacgccga cggtggccag gaaccacgcg
     3241 ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc ggccagccgg
     3301 gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc cgcttcgacg
     3361 ctctccggcg tggtccagac cgccaccgcg gtggcttaac tatgcggcat acgcgtgaaa
     3421 gacccccgct gacgggtagt caatcactca gaggagaccc tcccaaggaa cagcgagacc
     3481 acaagtcgga tgcaactgca agagggttta ttggatacac gggtacccgg gcgactcagt
     3541 caatcggagg actggcgccc cgagtgaggg gttgtgggct cttttgttga tgtctgggag
     3601 cagaagcgcg cgaacagaag cgagaagcta gcttgccaaa cctacaggtg gggtctttca
     3661 ttcccccctt tttctggaga ctaaataaaa tcttttattt tatctatggc tcgtactcta
     3721 taggcttcag ctggtgatat tgttgagtca aaactagagc ctggaccact gatatctccg
     3781 caggaagatc tcgacgtcag cttccccgac aacaccacgg aattatcagt gcccagcaac
     3841 ctagcccctg tccagcagcg ggcaaggcag gcggcgatga gttctgccgt ggcgataggg
     3901 agggggaaag cgaaagtccc agaaaggagt tgacaggtgg tggcaatgcc ccagccagtg
     3961 ggggttgcgt cagcaaacac agagcacacc acgccacgtt gacggacaac gggccacaac
     4021 tcctcataaa gagacagcaa ccaggattta tacaaggagg agaaaacgaa agccgtacgg
     4081 gaagcaatag catgatacag aggcattaaa gcagcatatc cacacagcgt aaaaggagca
     4141 acatagttmw gmmtrncwgk srakcytksr samwttyyry amycyarmkg wyrmwyawnc
     4201 arkwmttack yrktyasscr ssgrgcwtgy saaggtcaaa atcgtcaaga gcgtcagcag
     4261 gcagcatatc aaggtcaaag tcgtcaaggg catcggctgg gagcatgtct aagtcaaaat
     4321 cgtcaagggc gtcggtcggc ccgccgcttt cgcactttag ctgtttctcc aggccacata
     4381 tgattagttc caggccgaaa aggaaggcag gttcggctcc ctgccggtcg aacagctcaa
     4441 ttgcttgttt cagaagtggg ggcatagaat cggtggtagg tgtctctctt tcctcttttg
     4501 ctacttgatg ctcctgttcc tccaatacgc agcccagtgt aaagtggccc acggcggaca
     4561 gagcgtacag tgcgttctcc agggagaagc cttgctgaca caggaacgcg agctgatttt
     4621 ccagggtttc gtactgtttc tctgttgggc gggtgccgag atgcacttta gccccgtcgc
     4681 gatgtgagag gagagcacag cggtatgact tggcgttgtt ccgcagaaag tcttgccatg
     4741 actcgccttc cagggggcag gagtgggtat gatgcctgtc cagcatctcg attggcaggg
     4801 catcgagcag ggcccgcttg ttcttcacgt gccagtacag ggtaggctgc tcaactccca
     4861 gcttttgagc gagtttcctt gtcgtcaggc cttcgatacc gactccattg agtaattcca
     4921 gagcagagtt tatgactttg ctcttgtcca gtctagacat ggtaattcga tgatccccct
     4981 ggggagagag gtcggtgatt cggtcaacga gggagccgac tgccgacgtg cgctccggag
     5041 gcttgcagaa tgcggaacac cgcgcgggca ggaacagggc ccacactacc gccccacacc
     5101 ccgcctcccg caccgcccct tcccggccgc tgctctcggc gcgccctgct gagcagccgc
     5161 tattggccac agcccatcgc ggtcggcgcg ctgccattgc tccctggcgc tgtccgtctg
     5221 cgagggtact agtgagacgt gcggcttccg tttgtcacgt ccggcacgcc gcgaaccgca
     5281 aggaaccttc ccgacttagg ggcggagcag gaagcgtcgc cggggggccc acaagggtag
     5341 cggcgaagat ccgggtgacg ctgcgaacgg acgtgaagaa tgtgcgagac ccagggtcgg
     5401 cgccgctgcg tttcccggaa ccacgcccag agcagccgcg tccctgcgca aacccagggc
     5461 tgccttggaa aaggcgcaac cccaaccccg tggaattatc acctcgagtt tactccctat
     5521 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac
     5581 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga
     5641 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat
     5701 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtataagctt
     5761 tgcttatgta aaccagggcg cctataaaag agtgctgatt ttttgagtaa acttcaattc
     5821 cacaacactt ttgtcttata ccaactttcc gtaccacttc ctaccctcgt aaagaattcg
     5881 ccaccatgga ttacaaggat gacgacgata agcctggggc ctcgaagctc cgggcggttt
     5941 tggagaagtt gaagctcagc cgcgatgata tctccacggc ggcggggatg gtgaaagggg
     6001 ttgtggacca cctgctgctc agactgaagt gcgactccgc gttcagaggc gtcgggctgc
     6061 tgaacaccgg gagctactat gagcacgtga agatttctgc acctaatgaa tttgatgtca
     6121 tgtttaaact ggaagtcccc agaattcaac tagaagaata ttccaacact cgtgcatatt
     6181 actttgtgaa atttaaaaga aatccgaaag aaaatcctct gagtcagttt ttagaaggtg
     6241 aaatattatc agcttctaag atgctgtcaa agtttaggaa aatcattaag gaagaaatta
     6301 acgacattaa agatacagat gtcatcatga agaggaaaag aggagggagc cctgctgtaa
     6361 cacttcttat tagtgaaaaa atatctgtgg atataaccct ggctttggaa tcaaaaagta
     6421 gctggcctgc tagcacccaa gaaggcctgc gcattcaaaa ctggctttca gcaaaagtta
     6481 ggaagcaact acgactaaag ccattttacc ttgtacccaa gcatgcaaag gaaggaaatg
     6541 gtttccaaga agaaacatgg cggctatcct tctctcacat cgaaaaggaa attttgaaca
     6601 atcatggaaa atctaaaacg tgctgtgaaa acaaagaaga gaaatgttgc aggaaagatt
     6661 gtttaaaact aatgaaatac cttttagaac agctgaaaga aaggtttaaa gacaaaaaac
     6721 atctggataa attctcttct tatcatgtga aaactgcctt ctttcacgta tgtacccaga
     6781 accctcaaga cagtcagtgg gaccgcaaag acctgggcct ctgctttgat aactgcgtga
     6841 catactttct tcagtgcctc aggacagaaa aacttgagaa ttattttatt cctgaattca
     6901 atctattctc tagcaactta attgacaaaa gaagtaaaga atttctgaca aagcaaattg
     6961 aatatgaaag aaacaatgag tttccagttt ttgatgaatt ttacccatac gatgttccag
     7021 attacgcttg aggatccaga ccacctcccc tgcgagctaa gctggacagc caatgacggg
     7081 taagagagtg acatttttca ctaacctaag acaggagggc cgtcagagct actgcctaat
     7141 ccaaagacgg gtaaaagtga taaaaatgta tcactccaac ctaagacagg cgcagcttcc
     7201 gagggatttg agatccagac atgataagat acattgatga gtttggacaa accaaaacta
     7261 gaatgcagtg aaaaaaatgc cttatttgtg aaatttgtga tgctattgcc ttatttgtaa
     7321 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     7381 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     7441 ctgattatga tctagaggat ggtccacccc cggggtcggc agccttcacg tgggcggcgt
     7501 gtatccaagc tgcgatgccg tctactttga gggcggtggg ggtggtcagc aggactgtgt
     7561 aaggtccttt ccagcgaggt tctaggttct tagtctggtg tcggcggacc cacactgtgt
     7621 cgccgactcg gtaagggtga ggtaccaccg gtcggtccag ttgttcttgg taggctgccg
     7681 ccagaggtct ccagacttcg tgctggacta agtagagagc ctgtaagtga gcttggagag
     7741 aggggctgtt agtaactctt gtcatgtcag ggtcagggaa gtttacaagg ggcgggggtg
     7801 ccccatatag gatctcatat ggccatatgg gggcgcctag agaaggagtg agggctggat
     7861 aaagggagga tcgaggcggg gtcgaacgag gaggttcaag ggggagagac ggggcggatg
     7921 gaggaagagg aggcggaggc ttagggtgta caaagggctt gacccaggga ggggggtcaa
     7981 aagccaaggc ttcccaggtc acgatgtagg ggacctggtc tgggtgtcca tgcgggccag
     8041 gtgaaaagac cttgatctta acctgggtga tgaggtctcg gttaaaggtg ccgtctcgcg
     8101 gccatccgac gttaaaggtt ggccattctg cagagcagaa ggtaacccaa cgtctcttct
     8161 tgacatctac cgactggttg tgagcgatcc gctcgacatc tttccagtga cctaaggtca
     8221 aacttaaggg agtggtaaca ggctagcccg ggccctcatt ttcagacaaa tacagaaaca
     8281 cagtcagaca gagacaacac agaacgatgc tgcagcagac aagacgcgcg gcgcggcttc
     8341 ggtcccaaac cgaaagcaaa aattcagacg gaggcgggaa ctgttttagg ttctcgtctc
     8401 ctaccagaac cacatatccc tcctctaagg ggggtgcacc aaagagtcct aaacgatcgg
     8461 gattttagga ctcaggtcgg gccacaaaaa cggcccccga agtccctggg acgtctccca
     8521 gggttgcggc cgggtgttcc gaactcgtca gttccaccac gggtccgcca gatacagagc
     8581 tagttagcta actagtaccg acgcaggcgc ataaaatcag tcatagacac tagacaatcg
     8641 gacagacaca gataagttgc tggccagctt acctcccggt ggtgggtcgg tggtccctgg
     8701 gcaggggtct cccgatcccg gacgagcccc caaatgaaag acccccgctg acgggtagtc
     8761 aatcactcag aggagaccct cccaaggaac agcgagacca caagtcggat gcaactgcaa
     8821 gagggtttat tggatacacg ggtacccggg cgactcagtc aatcggagga ctggcgcccc
     8881 gagtgagggg ttgtgggctc ttttatagag ctcggggagc agaagcgctc tagatgccca
     8941 tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc
     9001 aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc
     9061 gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta
     9121 atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc
     9181 aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt
     9241 gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag
     9301 tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt
     9361 tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg
     9421 ggctatgaac taatgacccc gtaattgatt actattaata actagtcaat aatcaatgtc
     9481 aacatggcgg taatgttgga catgagccaa tataaatgta catattatga tatggataca
     9541 acgtatgcaa tgggccaagc tcctagggca tagaacaagt caaatgcttt atatatttga
     9601 gtttgggatg tattaggaaa ctcctaagag caaagctagc tt
//
