LOCUS       sequence-366535-        9855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing optimized Cas9 and NAT marker. Compatible with 
            CTG-clade yeast species..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Strucko T, Andersen NL, Mahler MR, Martinez JL, Mortensen UH
  TITLE     A CRISPR/Cas9 method facilitates efficient oligo-mediated gene 
            editing in Debaryomyces hansenii.
  JOURNAL   Synth Biol (Oxf). 2021 Oct 12;6(1):ysab031. doi: 
            10.1093/synbio/ysab031. eCollection 2021.
  PUBMED    34746438
REFERENCE   2  (bases 1 to 9855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-10-12"; volume: "6(1):ysab031. doi"; pages: " 
            10.1093/synbio/ysab031. eCollection 2021"
COMMENT     Sequence Label: pDIV492
FEATURES             Location/Qualifiers
     source          1..9855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    12..515
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     556..574
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             2121..6224
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2121..6224
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6237..6257
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6261..6281
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6285..6305
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6306..6371
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(6452..6470)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     terminator      6482..6670
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    6710..6743
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        6798..7141
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      7720..7917
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    7980..8013
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(8106..8694)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8186..8205)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8865..9656,9657..9725))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9488..9507
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9726..9830)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gggtcctttt catcacgtgc tataaaaata attataattt aaatttttta atataaatat
       61 ataaattaaa aatagaaagt aaaaaaagaa attaaagaaa aaatagtttt tgttttccga
      121 agatgtaaaa gactctaggg ggatcgccaa caaatactac cttttatctt gctcttcctg
      181 ctctcaggta ttaatgccga attgtttcat cttgtctgtg tagaagacca cacacgaaaa
      241 tcctgtgatt ttacatttta cttatcgtta atcgaatgta tatctattta atctgctttt
      301 cttgtctaat aaatatatat gtaaagtacg ctttttgttg aaatttttta aacctttgtt
      361 tatttttttt tcttcattcc gtaactcttc taccttcttt atttactttc taaaatccaa
      421 atacaaaaca taaaaataaa taaacacaga gtaaattccc aaattattcc atcattaaaa
      481 gatacgaggc gcgtgtaagt tacaggcaag cgatccgtct aagaaaccat tattatcatg
      541 acattaacct ataaaaatag gcgtatcacg aggccctttg aatgcgtgcg atgggcttag
      601 ttagccttgc ttgtgacttt gcagctttat ccatgaacgg atcatacgat tgtatttctg
      661 aatttgtcat agtgacttta agttgttctt tatttggtat gattctattt tttaggactt
      721 tctaaaaaat caatttaaat tttggaaaac catttgtggt ttttcttaaa tgattgcaaa
      781 attattaaaa aatcgttaaa aaaatattca gtgtaaaaag actagcattc atgatctcaa
      841 catctttgga taatatcaga atgagaaaga acagatacgc agtacgtttt ttggtgagct
      901 ctttgcactt ctttagttct ttccatcaat atcagttttt taaactttta ggactaaaag
      961 tgatgtttaa cttcaaaatg tttaaaattt tgttcttccc gacgttcatt aagaatacta
     1021 atacacttta ataattattt taatattttg ttctaaataa tgacttttaa ttaaaaaaga
     1081 taaaatataa aaacatcata ataactcacc agaggttaag aacaaaaaaa caaattagat
     1141 atctgctaat ccaatatagt taaatcaatc tttccttggt ataatgggta tattacatat
     1201 atttcaagga ccgacactcc taccaaatat ctaaaattta ccatattaac ataacatgta
     1261 tataaacgtc aaatcataat cagcactagc ggaatgcgtg cgatcgcgtg cattctcctc
     1321 ctttgggcct gtatcatggt ctttaagaat cataggacat tttactcgtt cccattgggc
     1381 cagtactgta ccaacactgt gcccacaata gatccagaat tattgtcgtc gcatgcgtcc
     1441 ttactaccgt acgtatatgt tctagagtag tatccaactc gtacctctag actacgagag
     1501 atccatcaca aatccgtggt agttccacag atccaagagg tccaagaaaa ttaaacctaa
     1561 ttttgacacc agcgctgcta tcgtttttcc gatcttcact gtaatatcga gctatcgtgg
     1621 gtattctcgt ggtccttcta aaaccgttgc aagcggtttt taaccacaaa aagaaataat
     1681 ggaacaattt ccaaaagaaa taaaattaca aaatcaaccg tgaattaccc gttttggtca
     1741 atttgtttcc gatcaattac gtaataggtt ttttatttct gttggccaca ataccaccgg
     1801 aacataactt tacactttta gagcgtatta tatggtaaca taccggaatc aagcgtatat
     1861 agcgcatcaa aagcatcgtt acgtaataga attttcaaga aaatggcgtt acgtaataag
     1921 atgatgataa ccaaaaaaaa aaatcgtatg ctagagtagt acattcagat agctagacat
     1981 ttctttttgt tgaaggaaat aattgatgaa tataaatacc gtggtaatcc ctgttacaat
     2041 ggataaataa aatatatatt tttacagtat tcaatacttt cattaataca aacataataa
     2101 aataaattac aatctgtcat atggataaaa agtatagtat tggtttagat attggtacta
     2161 actctgtggg ttgggcagtt atcactgacg aatataaagt tccatcaaag aaatttaagg
     2221 tgttaggtaa cactgacaga cactcaataa aaaagaatct tatcggtgct cttttgttcg
     2281 actccggtga aactgccgag gctacacgtt taaaaagaac agcaagaaga agatataccc
     2341 gtagaaaaaa tagaatatgt tatttacaag aaatcttttc taatgaaatg gctaaagttg
     2401 atgattcctt tttccataga ttggaagagt catttttggt tgaagaagac aaaaagcatg
     2461 agagacatcc aatctttggg aatatagttg atgaagtggc ttaccatgaa aaatatccta
     2521 ccatttatca tttaagaaag aaattggtag attcaactga taaagctgac cttagattaa
     2581 tctatttagc acttgcccat atgattaaat ttagaggtca ttttttgatt gaaggtgatt
     2641 tgaacccaga taattctgac gtggataaat tatttattca attagtccaa acctacaacc
     2701 aattatttga ggaaaatcca attaatgcta gtggtgtcga tgccaaagct atattatcag
     2761 ccagattatc aaaatctaga cgtttggaaa atttgattgc ccaattgcca ggagaaaaaa
     2821 agaatggatt atttggaaac ttgatcgcat tatcattggg tttgacacca aattttaaat
     2881 ctaattttga tttagctgaa gatgctaaat tacaattatc aaaagacacc tatgacgacg
     2941 atttggacaa tttacttgct caaattggtg atcaatatgc agatttgttc ttagctgcta
     3001 aaaacttatc tgatgctatt ttgttgtctg atattttgag agtgaacaca gaaataacca
     3061 aagctccatt atcagcatct atgatcaaac gttatgatga acaccatcag gatttgactt
     3121 tattgaaagc tttggtgaga caacaattgc cagagaagta taaagaaatc tttttcgatc
     3181 aatctaaaaa cgggtatgca ggttatattg atgggggtgc ctcccaagag gaattttaca
     3241 aatttataaa acctatttta gaaaagatgg atgggactga ggaacttttg gtcaaattga
     3301 acagagaaga tttgttacgt aaacagagaa cttttgataa tggtagtata cctcaccaaa
     3361 ttcatttggg tgagttgcat gcaattttaa gaagacaaga agatttttat ccatttttaa
     3421 aagataatag agaaaaaatc gagaaaattt taacctttag aattccatac tatgttgggc
     3481 ctttggctag aggtaattca agatttgcct ggatgacacg taaatcagaa gaaactatta
     3541 ccccttggaa ttttgaagag gttgttgata aaggagcatc agcacagagt tttattgaaa
     3601 gaatgaccaa tttcgataaa aacttaccaa atgaaaaagt tttaccaaaa cattccttgt
     3661 tatacgaata ttttactgtt tacaatgaac ttacaaaggt taaatatgtt actgaaggta
     3721 tgcgtaagcc agccttttta tctggagaac agaaaaaggc aatagttgat ttattgttta
     3781 aaacaaatag aaaagttact gttaaacaat taaaagaaga ttactttaag aaaattgaat
     3841 gttttgattc agttgaaatc agtggtgttg aagacagatt taatgctagt ttaggaactt
     3901 accatgattt acttaaaatt atcaaagata aagatttctt ggataacgaa gaaaatgaag
     3961 acattttaga agacattgtt ttaaccttaa ctttattcga agatagagag atgattgaag
     4021 aacgtttgaa gacttatgca catttgtttg acgataaagt gatgaaacag ttgaaaagaa
     4081 gacgttatac tggatggggt agattgtctc gtaaattgat caatggaatt agagataaac
     4141 aaagtggtaa aactatcttg gactttttga aatctgacgg atttgctaat agaaatttca
     4201 tgcaattgat ccacgacgat agtttgacat ttaaagaaga catccaaaag gcccaagtga
     4261 gtgggcaagg tgattcatta catgaacata ttgcaaattt agccggatct cctgctatta
     4321 agaaagggat attacaaact gttaaagttg tggatgaatt agtgaaagta atgggaagac
     4381 ataaacctga aaacattgtc attgagatgg caagagaaaa tcaaactaca caaaaaggac
     4441 agaaaaatag tagagaacgt atgaaaagaa tagaagaggg tattaaagaa ttgggtagtc
     4501 aaatattgaa agaacaccca gtggaaaata cccagttgca aaatgaaaaa ttatatcttt
     4561 actaccttca aaatggacgt gatatgtatg ttgatcagga attagatata aatagacttt
     4621 cagattatga tgtagatcat atagttccac aatctttctt gaaagatgat tccatagaca
     4681 ataaagtatt aactagaagt gataaaaata gaggtaaaag tgataatgtc ccaagtgagg
     4741 aagtcgtcaa aaagatgaaa aattactggc gtcaactttt gaatgctaaa ttaattactc
     4801 aaagaaaatt tgataatttg actaaagcag aaagaggtgg gctttctgaa ttagataaag
     4861 ccgggttcat taaaagacaa ttggtcgaaa ctagacaaat tactaaacat gttgcccaaa
     4921 ttttagattc ccgtatgaac actaagtatg acgaaaatga taagttaata cgtgaggtta
     4981 aagtcattac tttaaaatca aaacttgtct ctgatttcag aaaggatttc caattctata
     5041 aagttagaga aattaataat tatcatcatg ctcatgatgc atatttgaat gctgtagttg
     5101 gaactgcttt aatcaagaaa taccctaaat tagaatctga atttgtatat ggtgattaca
     5161 aagtctatga tgttagaaag atgattgcta aatcagaaca agaaattggt aaagctacag
     5221 ctaaatactt cttttactct aacattatga atttctttaa aacagaaatt actttggcaa
     5281 acggtgaaat tagaaaaaga cctcttattg aaacaaatgg tgagactgga gagatagttt
     5341 gggacaaagg gcgtgatttc gctactgtta gaaaagtttt atcaatgcca caagttaaca
     5401 ttgtaaagaa aacagaggtt caaactggtg gtttctcaaa agaaagtatt ttgcctaaaa
     5461 gaaatagtga taaattgatt gccagaaaaa aggattggga tccaaagaaa tatggtggtt
     5521 tcgactcacc aaccgtagcc tattctgttt tggttgtggc aaaggttgaa aagggtaaaa
     5581 gtaaaaagct taaatcagta aaagaacttt tgggtattac aataatggaa agaagttcct
     5641 ttgaaaagaa ccctattgat tttttggaag ctaaaggtta taaggaagta aagaaggact
     5701 taataatcaa attgcctaaa tattctttat ttgaattaga aaatgggaga aaaagaatgt
     5761 tggcttctgc tggagaattg caaaagggta atgaattagc attgccttcc aaatatgtta
     5821 acttcttgta tttagcttca cactatgaaa agttgaaagg gtcaccagaa gataacgagc
     5881 aaaaacaatt atttgttgaa caacacaaac actacttaga tgagattata gaacaaatta
     5941 gtgaattcag taaaagagtg atattagctg atgcaaattt agataaagtt ttgtcagcct
     6001 ataacaaaca tagagataag ccaattagag aacaagcaga aaacattatt cacttattta
     6061 cccttaccaa tttaggagca cctgctgctt tcaagtattt tgatacaaca attgatcgta
     6121 aaagatatac ctcaacaaaa gaagtcttag acgccacctt aattcatcaa tcaatcactg
     6181 gattgtatga gacaagaatt gatttgtctc aattgggtgg tgatgaaggg gctgatccta
     6241 agaagaaaag aaaagttgat ccaaagaaaa agcgtaaggt ggatcctaag aaaaagagaa
     6301 aggttgacta caaagaccat gacggtgatt ataaagatca tgacatcgac tacaaggatg
     6361 acgatgacaa gtgataatga ctgcagagat ccatcgacct gccgccaagc taattccggg
     6421 cgaatttctg tcgagtcatg taattagtta tgtcacgctt acattcacgc cctcccccca
     6481 catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt
     6541 tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc
     6601 tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg
     6661 gacgctcgaa atcgcgtgca ttcaacgctg caggtcgaca acccttaata taacttcgta
     6721 taatgtatgc tatacgaagt tattaggtct agagatctgt ttagcttgcc tcgtccccgc
     6781 cgggtcaccc ggccagcgac atggaggccc agaataccct ccttgacagt cttgacgtgc
     6841 gcagctcagg ggcatgatgt gactgtcgcc cgtacattta gcccatacat ccccatgtat
     6901 aatcatttgc atccatacat tttgatggcc gcacggcgcg aagcaaaaat tacggctcct
     6961 cgctgcagac ctgcgagcag ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg
     7021 ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc actgaggttc ttctttcata
     7081 tacttccttt taaaatcttg ctaggataca gttctcacat cacatccgaa cataaacaac
     7141 catgggaacc accttggatg acactgctta tcgataccga acatctgtac ctggtgatgc
     7201 tgaagctatc gaagcccttg acgggtcatt cacaactgac accgtcttta gagttacagc
     7261 tactggggat gggtttacat tgcgagaagt accagtagat cctcctttga ccaaagtatt
     7321 cccagatgac gaaagtgacg atgaatccga tgacggtgag gacggtgacc cagactcaag
     7381 aacatttgtc gcttatggag acgacggtga tttggccggt ttcgtagttg tatcctattc
     7441 tggatggaac cgaagactta ctgtcgagga tatagaggtt gccccagaac atcgtgggca
     7501 cggtgttggg cgagccctta tgggattagc tacagaattt gcccgtgaac gtggggctgg
     7561 acatttatgg ttagaagtca ccaatgttaa tgcaccagct atacatgcat atcgaagaat
     7621 ggggttcaca ctttgtggat tggatacagc tttatacgat ggtacagcct cagacggaga
     7681 acaagcttta tacatgtcta tgccttgtcc ataatcagta ctgacaataa aaagattctt
     7741 gttttcaaga acttgtcatt tgtatagttt ttttatattg tagttgttct attttaatca
     7801 aatgttagcg tgatttatat tttttttcgc ctcgacatca tctgcccaga tgcgaagtta
     7861 agtgcgcaga aagtaatatc atgcgttaat cgtatgtgaa tgctggtcgc tatactgctg
     7921 tcgattcgat actaacgccg ccatccagtg tcgaaaacga gctctcgaga acccttaata
     7981 taacttcgta taatgtatgc tatacgaagt tattaggtga tatcagatcc actagtggcc
     8041 attaagacct gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     8101 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     8161 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     8221 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     8281 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8341 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8401 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8461 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8521 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     8581 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8641 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8701 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     8761 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     8821 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8881 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8941 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     9001 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     9061 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     9121 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     9181 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     9241 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     9301 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     9361 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     9421 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     9481 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     9541 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     9601 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     9661 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     9721 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     9781 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9841 cgaaaagtgc cacct
//
