LOCUS       sequence-366547-        5118 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Xenopus laevis foxd4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Neilson KM, Klein SL, Mhaske P, Mood K, Daar IO, Moody SA
  TITLE     Specific domains of FoxD4/5 activate and repress neural 
            transcription factor genes to control the progression of immature 
            neural ectoderm to differentiating neural plate.
  JOURNAL   Dev Biol. 2012 May 15;365(2):363-75. doi: 
            10.1016/j.ydbio.2012.03.004. Epub 2012 Mar 10.
  PUBMED    22425621
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2012.03.004"; journalName: "Dev Biol"; date: 
            "2012-05-15- 15"; volume: "365"; issue: "2"; pages: "363-75"
COMMENT     Sequence Label: pCS2-foxd4l1.1-deltaN-myc
FEATURES             Location/Qualifiers
     source          1..5118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             103..132
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             142..171
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             181..210
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             220..249
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             259..288
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             322..351
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    1170..1304
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1194..1213)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1248..1267
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1417..1437)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1417..1435)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1456..1472)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1456..1472)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1469..1491)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1480..1496
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1504..1510,1511..1528,1529..1534))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1549..1570
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1687..1704)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1858..2446)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1938..1957)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2617..3408,3409..3477))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3240..3259
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3478..3582)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3608..4063)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3745..3766)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3957..3976
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4134..5118
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga tttaaagcta tggagcaaaa gctcatttct
      121 gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg
      181 gagcaaaagc tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa
      241 gaggacttga atgaaatgga gcaaaagctc atttctgaag aggacttgaa tgaaatggag
      301 agcttgggcg acctcaccat ggagcaaaag ctcatttctg aagaggactt gaattcaagg
      361 actctggtaa agcctcctta ctcttacatt gctctaatca ccatggctat cctgcagagt
      421 ccccacaaga aactgactct cagtggcatc tgtgatttta tcagcagcaa gttcccttac
      481 tacaaggaca agttccctgc ttggcagaat tccatcaggc acaacctgtc ccttaatgac
      541 tgctttatta aaataccacg ggaaccagga aatccaggaa aaggcaatta ttggacactg
      601 gaccctgctt ctgaggacat gtttgataac ggaagcttcc ttagaaggag gaaaaggttt
      661 aagaggcacc aacaggagtt ctttaaggat ggactcatga tgtacaactc tctgccttac
      721 tacagaccct acagtgctat ccaacctcag ccagtgctcc agcagacttc actgacatgc
      781 atggcaatac cagagacctt gcccatgtca actcaccttg caccctaccc agacataaag
      841 aggaaggttt cttaccctgc ccagggggta cacaggggtt ttaaagccca ggatgctgat
      901 aaccatccca ataattcaca gagcaaatgt tcattcagta ttgagaacat catgaggaaa
      961 cccaaggagc cagagccaaa tattcaaagc ttcaactcac actggaacta taatcatgta
     1021 ttccagaggc caagttcctg ccttctccca gctgtgctta acttatccac tggccctctg
     1081 ctagccaata ctcagggtgc caggcaatac aacctcatac aattccctgg gtgttactga
     1141 tctagaacta tagtgagtcg tattacgtag atccagacat gataagatac attgatgagt
     1201 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     1261 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     1321 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaattc gcggccgcgg
     1381 cgccaatgca ttgggcccgg tacccagctt ttgttccctt tagtgagggt taattgcgcg
     1441 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     1501 acacaacata cgagccggga gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     1561 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     1621 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     1681 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     1741 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     1801 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1861 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     1921 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1981 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2041 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2101 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2161 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2221 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2281 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2341 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2401 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2461 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     2521 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     2581 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     2641 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     2701 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     2761 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     2821 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     2881 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     2941 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     3001 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     3061 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     3121 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     3181 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     3241 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     3301 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     3361 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     3421 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     3481 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3541 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     3601 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     3661 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     3721 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     3781 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     3841 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     3901 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     3961 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     4021 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     4081 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagtcgacc
     4141 gccaattcaa tatggcgtat atggactcat gccaattcaa tatggtggat ctggacctgt
     4201 gccaattcaa tatggcgtat atggactcgt gccaattcaa tatggtggat ctggacccca
     4261 gccaattcaa tatggcggac ttggcaccat gccaattcaa tatggcggac ctggcactgt
     4321 gccaactggg gaggggtcta cttggcacgg tgccaagttt gaggaggggt cttggccctg
     4381 tgccaagtcc gccatattga attggcatgg tgccaataat ggcggccata ttggctatat
     4441 gccaggatca atatataggc aatatccaat atggccctat gccaatatgg ctattggcca
     4501 ggttcaatac tatgtattgg ccctatgcca tatagtattc catatatggg ttttcctatt
     4561 gacgtagata gcccctccca atgggcggtc ccatatacca tatatggggc ttcctaatac
     4621 cgcccatagc cactccccca ttgacgtcaa tggtctctat atatggtctt tcctattgac
     4681 gtcatatggg cggtcctatt gacgtatatg gcgcctcccc cattgacgtc aattacggta
     4741 aatggcccgc ctggctcaat gcccattgac gtcaatagga ccacccacca ttgacgtcaa
     4801 tgggatggct cattgcccat tcatatccgt tctcacgccc cctattgacg tcaatgacgg
     4861 taaatggccc acttggcagt acatcaatat ctattaatag taacttggca agtacattac
     4921 tattggaagt acgccagggt acattggcag tactcccatt gacgtcaatg gcggtaaatg
     4981 gcccgcgatg gctgccaagt acatccccat tgacgtcaat ggggaggggc aatgacgcaa
     5041 atgggcgttc cattgacgta aatgggcggt aggcgtgcct aatgggaggt ctatataagc
     5101 aatgctcgtt tagggaac
//
