LOCUS       sequence-366550-        4936 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Xenopus laevis foxd4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Neilson KM, Klein SL, Mhaske P, Mood K, Daar IO, Moody SA
  TITLE     Specific domains of FoxD4/5 activate and repress neural 
            transcription factor genes to control the progression of immature 
            neural ectoderm to differentiating neural plate.
  JOURNAL   Dev Biol. 2012 May 15;365(2):363-75. doi: 
            10.1016/j.ydbio.2012.03.004. Epub 2012 Mar 10.
  PUBMED    22425621
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2012.03.004"; journalName: "Dev Biol"; date: 
            "2012-05-15- 15"; volume: "365"; issue: "2"; pages: "363-75"
COMMENT     Sequence Label: pCS2-foxd4l1.1-deltaRII-Cterm
FEATURES             Location/Qualifiers
     source          1..4936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    988..1122
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1012..1031)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1066..1085
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1235..1255)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1235..1253)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1274..1290)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1274..1290)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1287..1309)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1298..1314
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1322..1328,1329..1346,1347..1352))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1367..1388
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1505..1522)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1676..2264)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1756..1775)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2435..3226,3227..3295))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3058..3077
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3296..3400)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3426..3881)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3563..3584)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3775..3794
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3952..4936
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattca aggcctatga gctttagcca
      121 ggagtctggt gcccatcatc acccccagga ttatgcagga ctttctgatg aggaggatga
      181 aattgatatt cttggagaag atgatccctg cagcctaaag tcacactttt acctacagcc
      241 cacacattca gtgatggggg acagtgagat gctgagtcct tccaagctca gctgcactga
      301 aagtgagagt gattcctctg gagagagtga aggaggtact tctaaagact cctcaaccac
      361 cccaactggt agcaaagcca aaaggactct ggtaaagcct ccttactctt acattgctct
      421 aatcaccatg gctatcctgc agagtcccca caagaaactg actctcagtg gcatctgtga
      481 ttttatcagc agcaagttcc cttactacaa ggacaagttc cctgcttggc agaattccat
      541 caggcacaac ctgtccctta atgactgctt tattaaaata ccacgggaac caggaaatcc
      601 gggaaaaggc aattattgga cactggaccc tgcttctgag gacatgtttg ataacggaag
      661 cttccttaga aggaggaaaa ggtttaagag gcaccaacag gagttcttta aggatggact
      721 catgatgtac aactctctgc cttactacag accctacagt gctatccaac ctcagccagt
      781 gctccagcag acttcactga catgcatggc aataccagag accttgccca tgtcaactca
      841 ccttgcaccc tacccagaca taaagaggaa ggtttcttac cctgcccagg gggtacacag
      901 gggttttaaa gcccaggatg ctgataacca tcccaataat tcacagagca aatgttgatc
      961 tagaactata gtgagtcgta ttacgtagat ccagacatga taagatacat tgatgagttt
     1021 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     1081 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     1141 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaattcgc ggccgcggcg
     1201 ccaatgcatt gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct
     1261 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     1321 acaacatacg agccgggagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     1381 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     1441 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     1501 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     1561 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     1621 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1681 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1741 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1801 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1861 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     1921 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1981 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2041 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2101 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2161 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2221 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2281 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     2341 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     2401 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     2461 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     2521 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     2581 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     2641 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     2701 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     2761 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     2821 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     2881 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     2941 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     3001 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     3061 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     3121 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     3181 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     3241 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     3301 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     3361 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     3421 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     3481 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     3541 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     3601 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     3661 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     3721 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     3781 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     3841 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
     3901 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agtcgaccgc
     3961 caattcaata tggcgtatat ggactcatgc caattcaata tggtggatct ggacctgtgc
     4021 caattcaata tggcgtatat ggactcgtgc caattcaata tggtggatct ggaccccagc
     4081 caattcaata tggcggactt ggcaccatgc caattcaata tggcggacct ggcactgtgc
     4141 caactgggga ggggtctact tggcacggtg ccaagtttga ggaggggtct tggccctgtg
     4201 ccaagtccgc catattgaat tggcatggtg ccaataatgg cggccatatt ggctatatgc
     4261 caggatcaat atataggcaa tatccaatat ggccctatgc caatatggct attggccagg
     4321 ttcaatacta tgtattggcc ctatgccata tagtattcca tatatgggtt ttcctattga
     4381 cgtagatagc ccctcccaat gggcggtccc atataccata tatggggctt cctaataccg
     4441 cccatagcca ctcccccatt gacgtcaatg gtctctatat atggtctttc ctattgacgt
     4501 catatgggcg gtcctattga cgtatatggc gcctccccca ttgacgtcaa ttacggtaaa
     4561 tggcccgcct ggctcaatgc ccattgacgt caataggacc acccaccatt gacgtcaatg
     4621 ggatggctca ttgcccattc atatccgttc tcacgccccc tattgacgtc aatgacggta
     4681 aatggcccac ttggcagtac atcaatatct attaatagta acttggcaag tacattacta
     4741 ttggaagtac gccagggtac attggcagta ctcccattga cgtcaatggc ggtaaatggc
     4801 ccgcgatggc tgccaagtac atccccattg acgtcaatgg ggaggggcaa tgacgcaaat
     4861 gggcgttcca ttgacgtaaa tgggcggtag gcgtgcctaa tgggaggtct atataagcaa
     4921 tgctcgttta gggaac
//
