LOCUS       sequence-366553-        5193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Xenopus laevis foxd4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Neilson KM, Klein SL, Mhaske P, Mood K, Daar IO, Moody SA
  TITLE     Specific domains of FoxD4/5 activate and repress neural 
            transcription factor genes to control the progression of immature 
            neural ectoderm to differentiating neural plate.
  JOURNAL   Dev Biol. 2012 May 15;365(2):363-75. doi: 
            10.1016/j.ydbio.2012.03.004. Epub 2012 Mar 10.
  PUBMED    22425621
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2012.03.004"; journalName: "Dev Biol"; date: 
            "2012-05-15- 15"; volume: "365"; issue: "2"; pages: "363-75"
COMMENT     Sequence Label: pCS2-foxd4l1.1-deltaRII-Cterm-myc
FEATURES             Location/Qualifiers
     source          1..5193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             103..132
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             142..171
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             181..210
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             220..249
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             259..288
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             322..351
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    1245..1379
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1269..1288)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1323..1342
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1492..1512)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1492..1510)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1531..1547)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1531..1547)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1544..1566)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1555..1571
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1579..1585,1586..1603,1604..1609))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1624..1645
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1762..1779)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1933..2521)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2013..2032)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2692..3483,3484..3552))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3315..3334
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3553..3657)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3683..4138)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3820..3841)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4032..4051
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4209..5193
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga tttaaagcta tggagcaaaa gctcatttct
      121 gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg
      181 gagcaaaagc tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa
      241 gaggacttga atgaaatgga gcaaaagctc atttctgaag aggacttgaa tgaaatggag
      301 agcttgggcg acctcaccat ggagcaaaag ctcatttctg aagaggactt gaattcaagg
      361 cctatgagct ttagccagga gtctggtgcc catcatcacc cccaggatta tgcaggactt
      421 tctgatgagg aggatgaaat tgatattctt ggagaagatg atccctgcag cctaaagtca
      481 cacttttacc tacagcccac acattcagtg atgggggaca gtgagatgct gagtccttcc
      541 aagctcagct gcactgaaag tgagagtgat tcctctggag agagtgaagg aggtacttct
      601 aaagactcct caaccacccc aactggtagc aaagccaaaa ggactctggt aaagcctcct
      661 tactcttaca ttgctctaat caccatggct atcctgcaga gtccccacaa gaaactgact
      721 ctcagtggca tctgtgattt tatcagcagc aagttccctt actacaagga caagttccct
      781 gcttggcaga attccatcag gcacaacctg tcccttaatg actgctttat taaaatacca
      841 cgggaaccag gaaatccggg aaaaggcaat tattggacac tggaccctgc ttctgaggac
      901 atgtttgata acggaagctt ccttagaagg aggaaaaggt ttaagaggca ccaacaggag
      961 ttctttaagg atggactcat gatgtacaac tctctgcctt actacagacc ctacagtgct
     1021 atccaacctc agccagtgct ccagcagact tcactgacat gcatggcaat accagagacc
     1081 ttgcccatgt caactcacct tgcaccctac ccagacataa agaggaaggt ttcttaccct
     1141 gcccaggggg tacacagggg ttttaaagcc caggatgctg ataaccatcc caataattca
     1201 cagagcaaat gttgatctag aactatagtg agtcgtatta cgtagatcca gacatgataa
     1261 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     1321 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     1381 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     1441 aattcgcggc cgcggcgcca atgcattggg cccggtaccc agcttttgtt ccctttagtg
     1501 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     1561 tccgctcaca attccacaca acatacgagc cgggagcata aagtgtaaag cctggggtgc
     1621 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     1681 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     1741 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1801 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1861 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1921 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1981 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2041 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2101 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2161 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2221 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2281 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2341 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2401 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2461 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2521 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2581 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2641 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     2701 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     2761 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     2821 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     2881 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     2941 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3001 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3061 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3121 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3181 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3241 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3301 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     3361 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     3421 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     3481 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     3541 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     3601 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     3661 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
     3721 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     3781 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     3841 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     3901 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     3961 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     4021 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     4081 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
     4141 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     4201 ttacgccagt cgaccgccaa ttcaatatgg cgtatatgga ctcatgccaa ttcaatatgg
     4261 tggatctgga cctgtgccaa ttcaatatgg cgtatatgga ctcgtgccaa ttcaatatgg
     4321 tggatctgga ccccagccaa ttcaatatgg cggacttggc accatgccaa ttcaatatgg
     4381 cggacctggc actgtgccaa ctggggaggg gtctacttgg cacggtgcca agtttgagga
     4441 ggggtcttgg ccctgtgcca agtccgccat attgaattgg catggtgcca ataatggcgg
     4501 ccatattggc tatatgccag gatcaatata taggcaatat ccaatatggc cctatgccaa
     4561 tatggctatt ggccaggttc aatactatgt attggcccta tgccatatag tattccatat
     4621 atgggttttc ctattgacgt agatagcccc tcccaatggg cggtcccata taccatatat
     4681 ggggcttcct aataccgccc atagccactc ccccattgac gtcaatggtc tctatatatg
     4741 gtctttccta ttgacgtcat atgggcggtc ctattgacgt atatggcgcc tcccccattg
     4801 acgtcaatta cggtaaatgg cccgcctggc tcaatgccca ttgacgtcaa taggaccacc
     4861 caccattgac gtcaatggga tggctcattg cccattcata tccgttctca cgccccctat
     4921 tgacgtcaat gacggtaaat ggcccacttg gcagtacatc aatatctatt aatagtaact
     4981 tggcaagtac attactattg gaagtacgcc agggtacatt ggcagtactc ccattgacgt
     5041 caatggcggt aaatggcccg cgatggctgc caagtacatc cccattgacg tcaatgggga
     5101 ggggcaatga cgcaaatggg cgttccattg acgtaaatgg gcggtaggcg tgcctaatgg
     5161 gaggtctata taagcaatgc tcgtttaggg aac
//
