LOCUS       sequence-366563-        5143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Xenopus laevis foxd4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Neilson KM, Klein SL, Mhaske P, Mood K, Daar IO, Moody SA
  TITLE     Specific domains of FoxD4/5 activate and repress neural 
            transcription factor genes to control the progression of immature 
            neural ectoderm to differentiating neural plate.
  JOURNAL   Dev Biol. 2012 May 15;365(2):363-75. doi: 
            10.1016/j.ydbio.2012.03.004. Epub 2012 Mar 10.
  PUBMED    22425621
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2012.03.004"; journalName: "Dev Biol"; date: 
            "2012-05-15- 15"; volume: "365"; issue: "2"; pages: "363-75"
COMMENT     Sequence Label: pCS2-foxd4l1.1-GARG
FEATURES             Location/Qualifiers
     source          1..5143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    1195..1329
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1219..1238)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1273..1292
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1442..1462)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1442..1460)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1481..1497)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1481..1497)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1494..1516)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1505..1521
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1529..1535,1536..1553,1554..1559))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1574..1595
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1712..1729)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1883..2471)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1963..1982)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2642..3433,3434..3502))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3265..3284
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3503..3607)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3633..4088)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3770..3791)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3982..4001
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4159..5143
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattca aggcctatga gctttagcca
      121 ggagtctggt gcccatcatc acccccagga ttatgcagga ctttctgatg aggaggatga
      181 aattgatatt cttggagaag atgatccctg cagcctaaag tcacactttt acctacagcc
      241 cacacattca gtgatggggg acagtgagat gctgagtcct tccaagctca gctgcactga
      301 aagtgagagt gattcctctg gagagagtga aggaggtact tctaaagact cctcaaccac
      361 cccaactggt agcaaagcca aaaggactct ggtaaagcct ccttactctt acattgctct
      421 aatcaccatg gctatcctgc agagtcccca caagaaactg actctcagtg gcatctgtga
      481 ttttatcagc agcaagttcc cttactacaa ggacaagttc cctgcttggc agaattccat
      541 caggcacaac ctgtccctta atgactgctt tattaaaata ccacgggaac caggaaatcc
      601 gggaaaaggc aattattgga cactggaccc tgcttctgag gacatgtttg ataacggaag
      661 cttccttaga aggaggaaaa ggtttaagag gcaccaacag gagttcttta aggatggact
      721 catgatgtac aactctctgc cttactacag accctacagt gctatccaac ctcagccagt
      781 gctccagcag acttcactga catgcatggc aataccagag accttgccca tgtcaactca
      841 ccttgcaccc tacccagaca taaagaggaa ggtttcttac cctgcccagg gggtacacag
      901 gggttttaaa gcccaggatg ctgataacca tcccaataat tcacagagca aatgttcatt
      961 cagtattgag aacatcatga ggaaacccaa ggagccagag ccaaatattc aaagcttcaa
     1021 ctcacactgg aactataatc atgtattcca gaggccaagt tcctgccttc tcccagctgt
     1081 gcttaactta tccactggcc ctctgctagc caatactcag ggtgccaggg gatacaacct
     1141 catacaattc cctgggtgtt actgatctag aactatagtg agtcgtatta cgtagatcca
     1201 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     1261 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     1321 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     1381 gaggtttttt aattcgcggc cgcggcgcca atgcattggg cccggtaccc agcttttgtt
     1441 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     1501 gaaattgtta tccgctcaca attccacaca acatacgagc cgggagcata aagtgtaaag
     1561 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     1621 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     1681 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     1741 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     1801 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     1861 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     1921 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     1981 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2041 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2101 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     2161 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     2221 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     2281 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     2341 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     2401 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2461 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     2521 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     2581 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     2641 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     2701 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     2761 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     2821 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     2881 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     2941 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     3001 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     3061 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     3121 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     3181 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     3241 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     3301 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     3361 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     3421 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     3481 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     3541 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     3601 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
     3661 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     3721 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     3781 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     3841 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     3901 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     3961 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     4021 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     4081 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     4141 ctcttcgcta ttacgccagt cgaccgccaa ttcaatatgg cgtatatgga ctcatgccaa
     4201 ttcaatatgg tggatctgga cctgtgccaa ttcaatatgg cgtatatgga ctcgtgccaa
     4261 ttcaatatgg tggatctgga ccccagccaa ttcaatatgg cggacttggc accatgccaa
     4321 ttcaatatgg cggacctggc actgtgccaa ctggggaggg gtctacttgg cacggtgcca
     4381 agtttgagga ggggtcttgg ccctgtgcca agtccgccat attgaattgg catggtgcca
     4441 ataatggcgg ccatattggc tatatgccag gatcaatata taggcaatat ccaatatggc
     4501 cctatgccaa tatggctatt ggccaggttc aatactatgt attggcccta tgccatatag
     4561 tattccatat atgggttttc ctattgacgt agatagcccc tcccaatggg cggtcccata
     4621 taccatatat ggggcttcct aataccgccc atagccactc ccccattgac gtcaatggtc
     4681 tctatatatg gtctttccta ttgacgtcat atgggcggtc ctattgacgt atatggcgcc
     4741 tcccccattg acgtcaatta cggtaaatgg cccgcctggc tcaatgccca ttgacgtcaa
     4801 taggaccacc caccattgac gtcaatggga tggctcattg cccattcata tccgttctca
     4861 cgccccctat tgacgtcaat gacggtaaat ggcccacttg gcagtacatc aatatctatt
     4921 aatagtaact tggcaagtac attactattg gaagtacgcc agggtacatt ggcagtactc
     4981 ccattgacgt caatggcggt aaatggcccg cgatggctgc caagtacatc cccattgacg
     5041 tcaatgggga ggggcaatga cgcaaatggg cgttccattg acgtaaatgg gcggtaggcg
     5101 tgcctaatgg gaggtctata taagcaatgc tcgtttaggg aac
//
