LOCUS       sequence-366574-        5400 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Xenopus laevis foxd4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Neilson KM, Klein SL, Mhaske P, Mood K, Daar IO, Moody SA
  TITLE     Specific domains of FoxD4/5 activate and repress neural 
            transcription factor genes to control the progression of immature 
            neural ectoderm to differentiating neural plate.
  JOURNAL   Dev Biol. 2012 May 15;365(2):363-75. doi: 
            10.1016/j.ydbio.2012.03.004. Epub 2012 Mar 10.
  PUBMED    22425621
REFERENCE   2  (bases 1 to 5400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2012.03.004"; journalName: "Dev Biol"; date: 
            "2012-05-15- 15"; volume: "365"; issue: "2"; pages: "363-75"
COMMENT     Sequence Label: pCS2-foxd4l1.1-GARP-myc
FEATURES             Location/Qualifiers
     source          1..5400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             103..132
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             142..171
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             181..210
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             220..249
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             259..288
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             322..351
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    1452..1586
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1476..1495)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1530..1549
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1699..1719)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1699..1717)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1738..1754)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1738..1754)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1751..1773)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1762..1778
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1786..1792,1793..1810,1811..1816))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1831..1852
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1969..1986)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2140..2728)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2220..2239)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2899..3690,3691..3759))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3522..3541
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3760..3864)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3890..4345)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4027..4048)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4239..4258
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4416..5400
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga tttaaagcta tggagcaaaa gctcatttct
      121 gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg
      181 gagcaaaagc tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa
      241 gaggacttga atgaaatgga gcaaaagctc atttctgaag aggacttgaa tgaaatggag
      301 agcttgggcg acctcaccat ggagcaaaag ctcatttctg aagaggactt gaattcaagg
      361 cctatgagct ttagccagga gtctggtgcc catcatcacc cccaggatta tgcaggactt
      421 tctgatgagg aggatgaaat tgatattctt ggagaagatg atccctgcag cctaaagtca
      481 cacttttacc tacagcccac acattcagtg atgggggaca gtgagatgct gagtccttcc
      541 aagctcagct gcactgaaag tgagagtgat tcctctggag agagtgaagg aggtacttct
      601 aaagactcct caaccacccc aactggtagc aaagccaaaa ggactctggt aaagcctcct
      661 tactcttaca ttgctctaat caccatggct atcctgcaga gtccccacaa gaaactgact
      721 ctcagtggca tctgtgattt tatcagcagc aagttccctt actacaagga caagttccct
      781 gcttggcaga attccatcag gcacaacctg tcccttaatg actgctttat taaaatacca
      841 cgggaaccag gaaatccggg aaaaggcaat tattggacac tggaccctgc ttctgaggac
      901 atgtttgata acggaagctt ccttagaagg aggaaaaggt ttaagaggca ccaacaggag
      961 ttctttaagg atggactcat gatgtacaac tctctgcctt actacagacc ctacagtgct
     1021 atccaacctc agccagtgct ccagcagact tcactgacat gcatggcaat accagagacc
     1081 ttgcccatgt caactcacct tgcaccctac ccagacataa agaggaaggt ttcttaccct
     1141 gcccaggggg tacacagggg ttttaaagcc caggatgctg ataaccatcc caataattca
     1201 cagagcaaat gttcattcag tattgagaac atcatgagga aacccaagga gccagagcca
     1261 aatattcaaa gcttcaactc acactggaac tataatcatg tattccagag gccaagttcc
     1321 tgccttctcc cagctgtgct taacttatcc actggccctc tgctagccaa tactcagggt
     1381 gccaggccat acaacctcat acaattccct gggtgttact gatctagaac tatagtgagt
     1441 cgtattacgt agatccagac atgataagat acattgatga gtttggacaa accacaacta
     1501 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     1561 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     1621 ttcaggggga ggtgtgggag gttttttaat tcgcggccgc ggcgccaatg cattgggccc
     1681 ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
     1741 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     1801 gagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     1861 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     1921 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     1981 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2041 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     2101 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2161 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2221 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2281 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2341 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2401 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2461 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     2521 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     2581 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     2641 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     2701 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     2761 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     2821 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     2881 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     2941 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     3001 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     3061 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     3121 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     3181 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     3241 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     3301 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     3361 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     3421 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     3481 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     3541 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     3601 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     3661 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     3721 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     3781 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     3841 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc
     3901 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     3961 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     4021 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     4081 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     4141 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     4201 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     4261 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     4321 cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa
     4381 gggcgatcgg tgcgggcctc ttcgctatta cgccagtcga ccgccaattc aatatggcgt
     4441 atatggactc atgccaattc aatatggtgg atctggacct gtgccaattc aatatggcgt
     4501 atatggactc gtgccaattc aatatggtgg atctggaccc cagccaattc aatatggcgg
     4561 acttggcacc atgccaattc aatatggcgg acctggcact gtgccaactg gggaggggtc
     4621 tacttggcac ggtgccaagt ttgaggaggg gtcttggccc tgtgccaagt ccgccatatt
     4681 gaattggcat ggtgccaata atggcggcca tattggctat atgccaggat caatatatag
     4741 gcaatatcca atatggccct atgccaatat ggctattggc caggttcaat actatgtatt
     4801 ggccctatgc catatagtat tccatatatg ggttttccta ttgacgtaga tagcccctcc
     4861 caatgggcgg tcccatatac catatatggg gcttcctaat accgcccata gccactcccc
     4921 cattgacgtc aatggtctct atatatggtc tttcctattg acgtcatatg ggcggtccta
     4981 ttgacgtata tggcgcctcc cccattgacg tcaattacgg taaatggccc gcctggctca
     5041 atgcccattg acgtcaatag gaccacccac cattgacgtc aatgggatgg ctcattgccc
     5101 attcatatcc gttctcacgc cccctattga cgtcaatgac ggtaaatggc ccacttggca
     5161 gtacatcaat atctattaat agtaacttgg caagtacatt actattggaa gtacgccagg
     5221 gtacattggc agtactccca ttgacgtcaa tggcggtaaa tggcccgcga tggctgccaa
     5281 gtacatcccc attgacgtca atggggaggg gcaatgacgc aaatgggcgt tccattgacg
     5341 taaatgggcg gtaggcgtgc ctaatgggag gtctatataa gcaatgctcg tttagggaac
//
