LOCUS       sequence-366630-       10327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP-tagged BRD protein, which can be used to be expressed in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10327)
  AUTHORS   Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M,
            Perez M, Agarwal P, Brodbelt JS, Legube G, Miller KM
  TITLE     Screen identifies bromodomain protein ZMYND8 in chromatin 
            recognition of transcription-associated DNA damage that promotes 
            homologous recombination.
  JOURNAL   Genes Dev. 2015 Jan 15;29(2):197-211. doi: 10.1101/gad.252189.114.
  PUBMED    25593309
REFERENCE   2  (bases 1 to 10327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.252189"; journalName: "Genes Dev"; date: "2015-01-15- 
            15"; volume: "29"; issue: "2"; pages: "197-211"
COMMENT     Sequence Label: GFP-BAZ1B
FEATURES             Location/Qualifiers
     source          1..10327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     206..226
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(235..663)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(345..366)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     557..576
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    865..913
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     938..957
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(997..1038)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    1046..1070
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1864..1875
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5523..5547)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(5563..6273,6274..6276,6277..6279))
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5605..5626)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5954..5973
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6213..6234
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6383..6407)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(6404..6607)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6433..6453)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6608..6987
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7159..7178
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7253..7357
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7358..7426,7427..8218)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7576..7595)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8389..8977
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8878..8897
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9131..9148
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9265..9286
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9301..9306,9307..9324,9325..9331)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9339..9355
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9344..9366
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9363..9379
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9363..9379
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    9428..9549
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9439..9458)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9493..9512
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(9707..10105)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(10124..10171)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(join(10219..10327,1..221))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(10233..10327,1..41)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg
       61 actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct
      121 ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct
      181 gctggggagc ctggggactt tccacaccct aactgacaca cattccacag aattaattcg
      241 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
      301 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
      361 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
      421 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
      481 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
      541 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
      601 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
      661 cgtggggata ccccctagag ccccagctgc gcagatctgc tatggcaggg cctgccgccc
      721 cgacgttggc tgcgagccct gggccttcac ccgaacttgg ggggtggggt ggggaaaagg
      781 aagaaacgcg ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc
      841 cctgggaccg aaccccgcgt ttatgaacaa acgacccaac acccgtgcgt tttattctgt
      901 ctttttattg ccgtcatagc gcgggttcct tccggtattg tctccttccg tgtttcagtt
      961 agcctccccc gtttaaactc attactaacc ggtacgcgta gaatcgagac cgaggagagg
     1021 gttagggata ggcttcccgt tatcaaccac tttgtacaag aaagctgggt ccttcttctg
     1081 tcttcgtccc ctggactgtc caacggcctc tggctcactg tccccttcat cttcagcaag
     1141 cctatcagga aacttcttgc gcttcctgcg gacatatggg tggccaggaa ggtgtttatg
     1201 caacagagcc actagacact gttctgtctt caccatgcag cttagcacat ggctgccacg
     1261 gcagttgtaa acctcagcat tggtaaacac ttgcttcatg tcagtaagaa actcctgcac
     1321 agagcggtag ctcccacagg aacatttgtt ctgcactgtc tgaaagtcca tggggtgcgt
     1381 gatcacatca tagtagtcct cggcctcatc tctggtcaca ggctccctga agggccagct
     1441 gaagcggtac ttcacgatct tgtggaggat ctcttcacac ttctgcagct ccaggctttg
     1501 cctccgggag ctccgcttgg tctgaagcac cagctcatcc acctcagcat catccacagg
     1561 tggtgccttg ggctgagacc tcctggtaga gtgtggcttc ttacccgggc gccggcctga
     1621 ccttgctgca ggggggatga cgctgtgctt gccccggatg gtctttcgag gtctcaatcg
     1681 caaaccagcc acctcataat cttcttcctc ctcctcctct tcttcctcct cctcctcttc
     1741 atcactctca tcatcttcac tgtcctcaga agcagactct tcagtatagt tcctgccacg
     1801 ggagttgcgc ctggcagtag cgggctggca agctgggcac tgccactcac catctggtac
     1861 ttcatagagg gccggcctca gacaaaacag gtggaaggct ttattacact catcacacaa
     1921 gatcaatttg tcatcctcac ctttctttcg acaaactttg cacctagcat tttctgcgga
     1981 catatcccac ttgatacagg catcaagcat cccaagcagc acgtgcatcc tggagaaagt
     2041 ctgagcttcc cggattgctg tcttccattt ctccagtgca gatgcaacct ttgcttcctc
     2101 taccattttc ttctcttcat ccacttcctc agtttttgct gaatcttcac tttggagttt
     2161 tcttctcttt tgcttgggag ccatgaagcc ttggagaaat ttctttatga cactggcctg
     2221 aagggcaatc acacactcac caaaatcctt caatttctct aatgaaatga cccgggcttc
     2281 aaattctgat gtttcttcca catatccaag tcctcctttt tgtaaccttg ttgcaacttc
     2341 aatgagatca ctacgaagga agtttaaaag ctcctggttg ccatcacaag attttagacc
     2401 caaatttggc ttccgtgcta gatgaataga gtgaataatg tcctggtacc tcttctctag
     2461 tctctcttta agttgacttt ctcttattcc ctgagggtga agacagttta gcaactcatc
     2521 cagctccttt tgactatcac ataaaaacca taggttctgt ccttgtttgg gtgtggttgt
     2581 ctctacagca acttctgttg ctgttccatg ttgtgtgttc atgcttgcat ttttacctaa
     2641 gtttgctttc ttactgcgag gacagtaatc ctcatcacca gagactgtgt ggtctttgca
     2701 gtgatggttg aatcggtagt caatgctgtc atgtacccag cctttttcaa tgaataatcc
     2761 tggaacttca tctgagaaga gccagtatct attatggttt cgatctgtgc caataggagt
     2821 cctgcgcatg actagtttgg ccttggcaat cccttcctgg aaagctttct cagcagctgc
     2881 tttgtgcttc cgtattcgtt cttcttcagc ttggcgttcc agtttcactt tcatttctct
     2941 ttcctggatt tcccgttcct tcttagcttg aatggcaagc aaccttctgc tcttcacagc
     3001 actaatcatg tcttcagctt ctgtatctac ttggggctca aacttcacaa tttctttttt
     3061 cctatcagtt ttgcctaacc cattctcaac ttttccattt tctttatttt tggcttccat
     3121 ttctttccgt ttctgtttct ctgctctctt cttatcattt tcttccttca acacagcaag
     3181 ccgttccttc cacaactctg cagacatctg ctgtctggtc tccatgtggt cttgcactga
     3241 gtatgtcatg aggatccggt ggcacagtgc tgtcaagatc tgtagcttct cctctgacgt
     3301 cagctcaaaa aattcagagg tctccagctt ttctaggaac tcatcttgta cctcattatc
     3361 ctcaaatgca gctgaatctt tattgtcatc tgtgtctgag ccctcgcttt cctcctgaac
     3421 atcagatctg cgcaagcaga gccgcaccag ctctgaaaca gaatgcagag tcaaggggat
     3481 ttccgacagc ttcattccca attcaccata gtcttctgct atctcatctt gtaggagggt
     3541 ctgtaagagg atgaccaaca ccctgttaag gtataaaaag ccacccttat ctgcactcaa
     3601 ggcttccata agggacacag cagtaatagg atactgagca tctggtaaaa gtagcccaga
     3661 ataacagctc aagaattcca ccaccatggc cacatcccca aacagcgtgt tgggcagccc
     3721 ttcaggggta tccaccaatc tgaatgctgg aaggtttttg ccagttaact cttggtcctc
     3781 ataccgcttc tgtttttcta atctctcaag catttctttc tctcttcgtt ctttggcttt
     3841 ttccttcaac ttctttttca gctcctcccg tttctttttc aaatattctt tccgttgttc
     3901 ttcagacata gaagcccacc tctttttgtg ctctagaagt tcatagcgtt tttgaacaag
     3961 acttcgcaat tcttctggga gacgagctct atcttcacta gagagaagac gagctgtttt
     4021 ggagataaca caggacaggg cgctcctctt gtcctccctg tctttgtttt ctttgtagta
     4081 tgcaatgagg tgtagggctg caggaggcag atgtttatga ggtttactag aggtcctagg
     4141 tgtaccccca gaattccgtg gggctcgtgt catcttctgc gtgcctttgg ccatatccaa
     4201 caaagtcatc tgcttcattt tggttttagg agtcttcagt ccttttttgg gagatttgga
     4261 attccctgtg gatttctgtc cattcaggat gcctttgctt ctgccctttg ccttcaaagg
     4321 cttgtcactg tgcttccctg gtttcttggc aggtgggcct tttttaggaa tgtgaaagtt
     4381 agtgtgcagc ttattgggcg acatcatctt catcatttct tctagatgtt cttcaggaga
     4441 tttggaattc tttgagttct tcactttgag tggcgagcca ctcaatgact tcttcaagtg
     4501 tacgtgacac cataacttag gatttagtgg tgaactaaga gaagagttgt ctgtcttgga
     4561 tttctttgag ggcttcctgt ctggggatcc agtattcttc ctcttagtag aagggttgag
     4621 agtcatatac ttgtatggat caagtaaaaa gtcactgaac ttgctgggca gagagtattt
     4681 cttcaccaat tcatcttcta cgacccaagg tgcattttca ccagtaccag ctcgtaatgc
     4741 attatgccgt ataaagtatc gaactatctc cttatttggt gggcgctctg tacgaatcaa
     4801 gctgtctgct ggcacgttac tgatgatctt atcttcattt tgtagtttca catcatattt
     4861 gtgaggcaga aattttggag gagcccattt cctttctcct ttttttaatg aagtaggaag
     4921 ttttcgtggc gatctacgtg ctctgtcatt aatactctct ctccttcctt catcctcttt
     4981 cacaactgtc tccttcttct gatggtcctg agcaatctga ctggagttct ctttgtcact
     5041 tgatggagaa tcacaggcac catcagattt cttctcagtg gcctcttcat ccactttctc
     5101 caaaggatga atcttcacaa tcttcacctt gagcattttc tccttcccaa cctcgaagtc
     5161 acactcttct cccacagcat atttggtcat gatctccaac caagcagtat ctactaactt
     5221 ctctaaggag gctgtgttat ggtgaaccat ttccagaaca agcttctcat accaggcagg
     5281 aaactcctcc ttcaaaagct cagcaacttc ctgttcttcc tcccaggctt ccttgtgtgt
     5341 tagctgactg cttccagtac tcttgcacgt ccaaatgcgc tcactgtacc tttccaagcg
     5401 ggcttcatac tcttcccggg tgcggaaggc ctcctgagtg tgcgggatgg tgaagagcgg
     5461 ctcctctccg ggcaacggct tcaccagcgg gaagggcttg cggcccagga gcggcgccat
     5521 gaagcctgct tttttgtaca aacttgttga tgggctcgag cccttgtaca gctcgtccat
     5581 gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc
     5641 gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac
     5701 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc
     5761 ctcgatgttg tggcgggtct tgaagttcac cttgatgccg ttcttctgct tgtcggcggt
     5821 gatatagacc ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc
     5881 ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
     5941 cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac
     6001 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
     6061 ggcgaagcac tgcacgccgt aggtgaaggt ggtcacgagg gtgggccagg gcacgggcag
     6121 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
     6181 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
     6241 caccaccccg gtgaacagct cctcgccctt gctcaccatg gtctcgagga tatcaccggt
     6301 agggatcctc tagagtccgg aggctggatc ggtcccggtg tcttctatgg aggtcaaaac
     6361 agcgtggatg gcgtctccag gcgatctgac ggttcactaa acgagctctg cttatataga
     6421 cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt
     6481 ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga
     6541 gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc
     6601 gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
     6661 ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag
     6721 ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat
     6781 actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca
     6841 ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa
     6901 gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact
     6961 attaataact agtcaataat caatgtcaac gcgtatatct ggcccgtaca tcgcgaagca
     7021 gcgcaaaacg cctaacccta agcagattct tcatgcaatt gtcggtcaag ccttgccttg
     7081 ttgtagctta aattttgctc gcgcactact cagcgacctc caacacacaa gcagggagca
     7141 gatactggct taactatgcg gcatcagagc agattgtact gagagtgcac cataggggat
     7201 cgggagatct cccgatccgt cgacgtcagg tggcactttt cggggaaatg tgcgcggaac
     7261 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7321 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7381 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     7441 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7501 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     7561 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     7621 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     7681 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     7741 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     7801 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     7861 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     7921 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     7981 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     8041 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     8101 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     8161 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     8221 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8281 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8341 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8401 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8461 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8521 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8581 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8641 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8701 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8761 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     8821 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     8881 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     8941 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9001 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     9061 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     9121 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     9181 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     9241 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     9301 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     9361 cacaggaaac agctatgacc atgattacgc caagctctag ctagaggtcg acggtataca
     9421 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     9481 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     9541 aaacaagttg gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca
     9601 gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc
     9661 gaaatctcgt agcacgtgtc agtcctgctc ctcggccacg aagtgcttag ccctcccaca
     9721 cataaccaga gggcagcaat tcacgaatcc caactgccgt cggctgtcca tcactgtcct
     9781 tcactatggc tttgatccca ggatgcagat cgagaagcac ctgtcggcac cgtccgcagg
     9841 ggctcaagat gcccctgttc tcatttccga tcgcgacgat acaagtcagg ttgccagctg
     9901 ccgcagcagc agcagtgccc agcaccacga gttctgcaca aggtccccca gtaaaatgat
     9961 atacattgac accagtgaag atgcggccgt cgctagagag agctgcgctg gcgacgctgt
    10021 agtcttcaga gatggggatg ctgttgattg tagccgttgc tctttcaatg agggtggatt
    10081 cttcttgaga caaaggcttg gccatggttt agttcctcac cttgtcgtat tatactatgc
    10141 cgatatacta tgccgatgat taattgtcaa cacgtgctga tcagatccga aaatggatat
    10201 acaagctccc gggagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
    10261 ctggaatagc tcagaggcag aggcggcctc ggcctctgca taaataaaaa aaattagtca
    10321 gccatgg
//
