LOCUS       sequence-36665-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Chao JA, Patskovsky Y, Almo SC, Singer RH
  TITLE     Structural basis for the coevolution of a viral RNA-protein complex.
  JOURNAL   Nat Struct Mol Biol. 2008 Jan;15(1):103-5. Epub 2007 Dec 9.
  PUBMED    18066080
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol."; date: "2008-01"; volume: "15(1)"; pages: "103-5. Epub 
            2007 Dec 9"
COMMENT     Sequence Label: pET22HT-PP7delFG
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..90
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             484..501
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(554..572)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      568..615
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(739..758)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attccctctg aatattttgt ttaactttaa gaaggagata tacatatgca tcatcatcat
       61 catcacggcg aaaacctgta ttttcagggc ggatccatgt ccaaaaccat cgttctttcg
      121 gtcggcgagg ctactcgcac tctgactgag atccagtcca ccgcagaccg tcagatcttc
      181 gaagagaagg tcgggcctct ggtgggtcgg ctgcgcctca cggcttcgct ccgtcaaaac
      241 ggagccaaga ccgcgtatcg cgtcaaccta aaactggatc aggcggacgt cgttgattcc
      301 ggacttccga aagtgcgcta cactcaggta tggtcgcacg acgtgacaat cgttgcgaat
      361 agcaccgagg cctcgcgcaa atcgttgtac gatttgacca agtccctcgt cgcgacctcg
      421 caggtcgaag atcttgtcgt caaccttgtg ccgctgggcc gttgataagc ggccgcactc
      481 gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag
      541 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
      601 ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct
      661 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
      721 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
      781 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
      841 ggcacctcga ccccaaaaaa cttgattagg g
//
