LOCUS       sequence-366671-        4857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Xenopus laevis foxd4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Yan B, Neilson KM, Moody SA
  TITLE     foxD5 plays a critical upstream role in regulating neural ectodermal
            fate and the onset of neural differentiation.
  JOURNAL   Dev Biol. 2009 May 1;329(1):80-95. doi: 10.1016/j.ydbio.2009.02.019.
            Epub 2009 Feb 26.
  PUBMED    19250931
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2009.02.019"; journalName: "Dev Biol"; date: 
            "2009-05-1- 1"; volume: "329"; issue: "1"; pages: "80-95"
COMMENT     Sequence Label: pCS2-foxd4l1.1-EnR
FEATURES             Location/Qualifiers
     source          1..4857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    909..1043
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(933..952)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     987..1006
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1156..1176)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1156..1174)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1195..1211)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1195..1211)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1208..1230)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1219..1235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1243..1249,1250..1267,1268..1273))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1288..1309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1426..1443)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1597..2185)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1677..1696)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2356..3147,3148..3216))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2979..2998
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3217..3321)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3347..3802)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3484..3505)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3696..3715
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3873..4857
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga tttaaagcta tgctggtaaa gcctccttac
      121 tcttacattg ctctaatcac catggctatc ctgcagagtc cccacaagaa actgactctc
      181 agtggcatct gtgattttat cagcagcaag ttcccttact acaaggacaa gttccctgct
      241 tggcagaatt ccatcaggca caacctgtcc cttaatgact gctttattaa aataccacgg
      301 gaaccaggaa atccaggaaa aggcaattat tggacactgg accctgcttc tgaggacatg
      361 tttgataacg gaagcttcct tagaaggagg aaaaggttta agaggcacca acaggagttc
      421 tttaaggatg gactcatgat gtacaactct ctgccttact acagacccta cagtgctatc
      481 caacctcagc cagtgctcca gcagacttca ctgacatgca tggcaatacc agagaccttg
      541 cccatgtcaa ctcaccttgc accctaccca gacataaaga ggaaggtttc ttaccctgcc
      601 cagggggtac acaggggttt taaagcccag gatgctgata accatcccaa taattcacag
      661 agcaaatgtt cattcagtat tgagaacatc atgaggaaac ccaaggagcc agagccaaat
      721 attcaaagct tcaactcaca ctggaactat aatcatgtat tccagaggcc aagttcctgc
      781 cttctcccag ctgtgcttaa cttatccact ggccctctgc tagccaatac tcagggtgcc
      841 aggcaataca acctcataca attccctggg tgttactgat ctagaactat agtgagtcgt
      901 attacgtaga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
      961 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     1021 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     1081 agggggaggt gtgggaggtt ttttaattcg cggccgcggc gccaatgcat tgggcccggt
     1141 acccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
     1201 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccgggag
     1261 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     1321 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     1381 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     1441 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1501 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1561 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     1621 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1681 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1741 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1801 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1861 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1921 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1981 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     2041 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2101 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2161 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2221 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     2281 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     2341 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     2401 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     2461 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     2521 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     2581 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     2641 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     2701 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     2761 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     2821 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     2881 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     2941 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     3001 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     3061 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     3121 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     3181 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     3241 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     3301 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt
     3361 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     3421 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     3481 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     3541 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     3601 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     3661 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     3721 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     3781 aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg
     3841 cgatcggtgc gggcctcttc gctattacgc cagtcgaccg ccaattcaat atggcgtata
     3901 tggactcatg ccaattcaat atggtggatc tggacctgtg ccaattcaat atggcgtata
     3961 tggactcgtg ccaattcaat atggtggatc tggaccccag ccaattcaat atggcggact
     4021 tggcaccatg ccaattcaat atggcggacc tggcactgtg ccaactgggg aggggtctac
     4081 ttggcacggt gccaagtttg aggaggggtc ttggccctgt gccaagtccg ccatattgaa
     4141 ttggcatggt gccaataatg gcggccatat tggctatatg ccaggatcaa tatataggca
     4201 atatccaata tggccctatg ccaatatggc tattggccag gttcaatact atgtattggc
     4261 cctatgccat atagtattcc atatatgggt tttcctattg acgtagatag cccctcccaa
     4321 tgggcggtcc catataccat atatggggct tcctaatacc gcccatagcc actcccccat
     4381 tgacgtcaat ggtctctata tatggtcttt cctattgacg tcatatgggc ggtcctattg
     4441 acgtatatgg cgcctccccc attgacgtca attacggtaa atggcccgcc tggctcaatg
     4501 cccattgacg tcaataggac cacccaccat tgacgtcaat gggatggctc attgcccatt
     4561 catatccgtt ctcacgcccc ctattgacgt caatgacggt aaatggccca cttggcagta
     4621 catcaatatc tattaatagt aacttggcaa gtacattact attggaagta cgccagggta
     4681 cattggcagt actcccattg acgtcaatgg cggtaaatgg cccgcgatgg ctgccaagta
     4741 catccccatt gacgtcaatg gggaggggca atgacgcaaa tgggcgttcc attgacgtaa
     4801 atgggcggta ggcgtgccta atgggaggtc tatataagca atgctcgttt agggaac
//
