LOCUS       sequence-36669-e         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  TITLE     pBabe-IRES-EGFP plasmids
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBabe neo IRES-EGFP
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(39..60)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(195..707,708..710,711..713))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     388..407
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     647..668
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 antnnagctg tcatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg
       61 tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg
      121 tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc
      181 tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc
      241 tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc
      301 aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg
      361 tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg
      421 gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
      481 tggtcggggt agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc
      541 cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg
      601 gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc
      661 tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac catggttgtg
      721 gccatattat catcgtgttt ttcaaaggaa aaccacgtcc ccgtggttcg gggggcctag
      781 acgttttttt aacctcgact aaacacatgt aaagcatgtg taccgaggcc ccanatcaga
      841 tcccatacaa atggggtacc ttctgggcat ccttcanccc cttgttgaat acgcttngag
      901 gagagcnatt tgactctttc cacaactatc caactcanaa cgtggcactg gggttgtgcn
      961 cttnaggata antgttgca
//
