LOCUS       sequence-36675-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Kazgan N, Williams T, Forsberg LJ, Brenman JE
  TITLE     Identification of a nuclear export signal in the catalytic subunit 
            of AMP-activated protein kinase.
  JOURNAL   Mol Biol Cell. 2010 Oct 1. 21(19):3433-42.
  PUBMED    20685962
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2010 Oct 1. 21(19):3433-42."
COMMENT     Sequence Label: pFLAG-Cherry-N1-PRKAG1
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             246..269
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    393..514
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(430..449)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     484..503
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(658..679)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     870..889
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gagtccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
       61 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
      121 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
      181 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
      241 acaaggacta taaggacgat gatgacaagt agcggccgcg actctagatc ataatcagcc
      301 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
      361 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
      421 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
      481 gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt
      541 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
      601 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
      661 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
      721 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
      781 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
      841 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc g
//
