LOCUS       sequence-36678-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Barde I, Laurenti E, Verp S, Wiznerowicz M, Offner S, Viornery A, 
            Galy A, Trumpp A, Trono D
  TITLE     Lineage- and stage-restricted lentiviral vectors for the gene 
            therapy of chronic granulomatous disease.
  JOURNAL   Gene Ther. 2011 Nov;18(11):1087-97. doi: 10.1038/gt.2011.65. Epub 
            2011 May 5.
  PUBMED    21544095
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/gt.2011.65"; journalName: "Gene Ther"; date: "2011-11"; 
            volume: "18"; issue: "11"; pages: "1087-97"
COMMENT     Sequence Label: pRRL.gp91.EF1aIntron.eGFP
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          89..292
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(112..132)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggtagcgc taggacgcgt aagcttaggc
       61 ctggatcccg gggtagtttt cacgacacct gaaatggaag aaaaaaactt tgaaccactg
      121 tctgaggctt gagaatgaac caagatccaa actcaaaaag ggcaaattcc aaggagaatt
      181 acatcaagtg ccaagctggc ctaacttcag tctccaccca ctcagtgtgg ggaaactcca
      241 tcgcataaaa cccctccccc caacctaaag acgacgtact ccaaaagctc gagaactaat
      301 cgaggtgcct ggacggcgcc cggtactccg tggagtcaca tgaagcgacg gctgaggacg
      361 gaaaggccct tttcctttgt gtgggtgact cacccgcccg ctctcccgag cgccgcgtcc
      421 tccattttga gctccctgca gcagggccgg gaagcggcca tctttccgct cacgcaactg
      481 gtgccgaccg ggccagcctt gccgcccagg gcggggcgat acacggcggc gcgaggccag
      541 gcaccagagc aggccggcca gcttgagact acccccgtcc gattctcggt ggccgcgctc
      601 gcaggccccg cctcgccgaa catgtgcgct gggacgcacg ggccccgtcg ccgcccgcgg
      661 ccccaaaaac cgaaatacca gtgtgcagat cttggcccgc atttacaaga ctatcttgcc
      721 agaaaaaaag cgtcgcagca ggtcatcaaa aattttaaat ggctagagac ttatcgaaag
      781 cagcgagaca ggcgcgaagg tgccaccaga ttcgcacgcg gcggccccag cgcccaggcc
      841 aggcctcaac tcaagcacga ggcgaagggg ctccttaagc gcaaggcctc gaactctccc
      901 acccacttcc aacccgaagc tcgg
//
