LOCUS       sequence-366785-       10213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tier-3 vector for CRISPR-mediated stable integration into the AAVS1 
            locus of the tetON-system 
            (OTetO7-PCMVmin-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::A3-pA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10213)
  AUTHORS   Haellman V, Strittmatter T, Bertschi A, Stucheli P, Fussenegger M
  TITLE     A versatile plasmid architecture for mammalian synthetic biology 
            (VAMSyB).
  JOURNAL   Metab Eng. 2021 Jul;66:41-50. doi: 10.1016/j.ymben.2021.04.003. Epub
            2021 Apr 18.
  PUBMED    33857582
REFERENCE   2  (bases 1 to 10213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2021-07"; volume: "66:41-50. doi"; pages: " 
            10.1016/j.ymben.2021.04.003. Epub 2021 Apr 18"
COMMENT     Sequence Label: pTS2163
FEATURES             Location/Qualifiers
     source          1..10213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     60..79
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             180..920
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="SRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTTD
                     SMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPAD
                     ALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(1042..1058)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1042..1058)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1214..1235
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    1290..2126
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     rep_origin      complement(2217..2805)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2297..2316)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2926..3717,3718..3786))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3549..3568
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3787..3891)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    3970..4773
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    4780..4805
                     /label=SA
                     /note="splice acceptor site"
     CDS             4829..4882
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5209..5220
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             5642..5698
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     polyA_signal    6338..6459
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6349..6368)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6403..6422
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    6497..6515
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6533..6551
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6569..6587
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6605..6623
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6641..6659
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6677..6695
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6713..6731
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     6785..6809
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     6861..6880
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6861..6879
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             8510..8566
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8573..9502
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="ARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAGA
                     FFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLHR
                     HDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVML
                     YRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSRI
                     VPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYEP
                     RAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     primer_bind     complement(9563..9580)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9569..9793
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        join(9841..10213,1..127)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9876..9899)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
       61 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
      121 cctttcgacc tccagcccga gctcggtata gggagaccga attcaccatg actagtggat
      181 caagactgga caagagcaaa gtcataaact ctgctctgga attactcaat ggagtcggta
      241 tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt
      301 actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca
      361 ggcatcatac ccactcctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca
      421 acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg
      481 gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc
      541 agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac
      601 tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta
      661 ccaccgattc tatgccccca cttctgaaac aagcaattga gctgttcgac cggcagggag
      721 ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa
      781 agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag
      841 ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg
      901 accttgacat gctccccggg gctagcggat ccaccggtgt ctagaaagct atacaggtgc
      961 accgctttat aataaaactg atacagagaa cgttatcatg tgtgttggtt ttttttgtgt
     1021 gtgcggttta agggagcctc ggtcatagct gtttcctgtg taaaacgacg gccagcgcgg
     1081 gagacggtca gttcgtctcc agctcagaaa ttatgagagc gtttaataaa gtattgacta
     1141 tggttgacaa aacgtgtgtt ggagtttttt gtgtgctttg tcatcaactg agccagtagg
     1201 tcgagggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     1261 tccagtcggg aaacctgtcg tgccagctga ctagggacag gattggtgac agaaaagccc
     1321 catccttagg cctcctcctt cctagtctcc tgatattggg tctaaccccc acctcctgtt
     1381 aggcagattc cttatctggt gacacacccc catttcctgg agccatctct ctccttgcca
     1441 gaacctctaa ggtttgctta cgatggagcc agagagaatc ctgggaggga gagcttggca
     1501 gggggtggga gggaaggggg ggatgcgtga cctgcccggt tctcagtggc caccctgcgc
     1561 taccctctcc cagaacctga gctgctctga cgcggctgtc tggtgcgttt cactgatcct
     1621 ggtgctgcag cttccttaca cttcccaaga ggagaagcag tttggaaaaa caaaatcaga
     1681 ataagttggt cctgagttct aactttggct cttcaccttt ctagtcccca atttatattg
     1741 ttcctccgtg cgtcagtttt acctgtgaga taaggccagt agccagcccc gtcctggcag
     1801 ggctgtggtg aggagggggg tgtccgtgtg gaaaactccc tttgtgagaa tggtgcgtct
     1861 taggtgttca ccaggtcgtg gccgcctcta ctccctttct ctttctccat ccttctttcc
     1921 ttaaagagtc cccagtgcta tctgggacat attcctccgc ccagagcagg gtcccgcttc
     1981 cctaaggccc tgctctgggc ttctgggttt gagtccttgg caagcccagg agaggcgctc
     2041 aggcttccct gtcccccttc ctcgtccacc atctcatgcc cctggctctc ctgccccttc
     2101 cctacagggg ttcctggctc tgctctccaa tcctgtccct agtggccccc catggacatg
     2161 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     2221 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     2281 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     2341 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     2401 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     2461 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     2521 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     2581 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     2641 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     2701 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     2761 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     2821 ttttctacgg ggattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     2881 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     2941 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     3001 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     3061 accgcggctc ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     3121 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     3181 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     3241 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     3301 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     3361 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     3421 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     3481 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     3541 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     3601 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     3661 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     3721 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     3781 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     3841 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     3901 ccgaaaagtg ccacctgacg tctaagaaac caagatcagc ggccgcgggg ccactaggga
     3961 caggattggt gctttctctg accagcattc tctcccctgg gcctgtgccg ctttctgtct
     4021 gcagcttgtg gcctgggtca cctctacggc tggcccagat ccttccctgc cgcctccttc
     4081 aggttccgtc ttcctccact ccctcttccc cttgctctct gctgtgttgc tgcccaagga
     4141 tgctctttcc ggagcacttc cttctcggcg ctgcaccacg tgatgtcctc tgagcgaatc
     4201 ctccccgtgt ctgggtcctc tccgggcatc tctcctccct cacccaaccc catgccgtct
     4261 tcactcgctg ggttcccttt tccttctcct tctggggcct gtgccatctc tcgtttctta
     4321 ggatggcctt ctccgacgga tgtctccctt gcgtcccgcc tccccttctt gtaggcctgc
     4381 atcatcaccg tttttctgga caaccccaaa gtaccccatc tccctggctt tagccacctc
     4441 tccatcctct tgctttcttt gcctggacac cccgttctcc tgtggattcg ggtcacctct
     4501 cactcctttc atttgggcag ctcccctacc ccccttacct ctctagtctg tgctaactct
     4561 tccagccccc tgtcatggca tcttccaggg gtccgagagt tcagctagtc ctcttcctcc
     4621 aacccgggcc cctatgtcca cttcaggaca gcatgtttgc tgcctccagg aatcctgtgt
     4681 ccccgagctg ggaccacctt atattcccag ggccggttaa tgtggctctg gttctgggta
     4741 cttttatctg tcccctccac cccacagtgg ggcaagctac tgacctcttc tcttcctccc
     4801 acagggccct tcaggcggcg gacccgggga gggcagagga agccttctaa catgcggtga
     4861 cgtggaggag aatcccggcc ctgctagtgg tggtcaattg gtgtctaaag gcgaggagct
     4921 atttacagga gtcgtgccca ttctagtcga attggatggt gacgttaacg gccacaaatt
     4981 ctctgtttcg ggagagggtg aaggtgatgc cacttacggc aagttaactc tgaagttgtt
     5041 atgtacaaca gggaagctcc ccgtcccctg gccaaccctc gttacaacac tcgggtacgg
     5101 agtacaatgc tttgctaggt atccagatca tatgaagcaa cacgattttt ttaaatccgc
     5161 tatgcccgaa ggctatgtac aggaacgtac catttttttt aaggatgatg ggaattacaa
     5221 gacacgagcg gaagttaaat ttgagggcga caccctagtg aatcgtattg agcttaaggg
     5281 catagatttt aaggaggacg gtaatatact cggtcataag ctagagtata attataattc
     5341 tcataatgtg tatatcacgg ccgataaaca gaaaaacggg atcaaggcca atttcaagat
     5401 caggcataat atcgaggacg gtggggtcca actggctgat cattaccagc agaacacgcc
     5461 gatcggtgac ggccctgttc ttctgcccga taaccactac ctctcatacc agagcgcact
     5521 tttcaaagat cccaacgaga agagagatca catggtactg ttggaatttc tcaccgcggc
     5581 aggtatcaca gaggggatgg acgaactgta caaggcctcc ggaggaagcg gaggaagtgg
     5641 cgccacaaac ttctcactct tgaaacaggc aggtgacgtc gaagaaaacc cagggcccgc
     5701 atccggtggg agtgggacag agtataagcc aacggttagg cttgcgacgc gggatgatgt
     5761 ccccagggcc gttcgcactc ttgcggcagc attcgctgac taccctgcca ccaggcacac
     5821 tgtagaccct gaccggcaca ttgaaagagt gactgaactg caggaacttt tcctgactcg
     5881 cgtcggcttg gacatcggca aggtctgggt ggctgacgac ggcgccgccg tggcagtgtg
     5941 gactaccccc gaaagtgtgg aagctggggc cgtgttcgct gaaattggac caagaatggc
     6001 tgagctgagc ggatcacggc tggctgcaca gcagcagatg gaggggctgc tggcccccca
     6061 cagaccgaaa gagcctgcat ggttcctggc aaccgtgggc gtgagcccag accaccaggg
     6121 gaaaggactg ggctccgccg tggtgctgcc aggcgtggag gcagccgaga gagctggcgt
     6181 gccagccttc ctggaaacct ctgctcctcg aaacctgcct ttctatgagc gcctgggctt
     6241 cacagtgacc gccgacgtgg aggtgccaga gggccctcgg acctggtgca tgacccgcaa
     6301 acctggagcc tgacctaggt gatcttgaca gacatgataa gatacattga tgagtttgga
     6361 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     6421 gctttatttg taaccattat aagctgcaat aaacaagtta gatctacgcg tccgtacacg
     6481 cctaaagcat atacgttctc tatcactgat agggagtaaa ctggatatac gttctctatc
     6541 actgataggg agtaaactgt agatacgttc tctatcactg atagggagta aactggtcat
     6601 acgttctcta tcactgatag ggagtaaact ccttatacgt tctctatcac tgatagggag
     6661 taaagtctgc atacgttctc tatcactgat agggagtaaa ctcttcatac gttctctatc
     6721 actgataggg agtaaactcg agtatgtcga ggtggcgtgt acggtgggag gcctatataa
     6781 gcagagctcg tttagtgaac cgtcagatcg cgagctctct ggctaactag agaacccact
     6841 gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc tgttcgaagc
     6901 ggaattcacc atgactagtt ggctgtggct ggtgctgctg ctgctgtgcc tggccggcaa
     6961 cgtgcaggcc gctagtatca tccccgtgga ggaggagaac cccgacttct ggaacagaga
     7021 ggccgccgag gccctgggcg ccgccaagaa gctgcagccc gcccagaccg ccgccaagaa
     7081 cctgatcatc ttcctgggcg acggcatggg cgtgagcacc gtgaccgccg ccagaatcct
     7141 gaagggccag aagaaggaca agctgggccc cgagatcccc ctggccatgg acagattccc
     7201 ctacgtggcc ctgagcaaga cctacaacgt ggacaagcac gtgcccgaca gcggcgccac
     7261 cgccaccgcc tacctgtgcg gcgtgaaggg caacttccag accatcggcc tgagcgccgc
     7321 cgccagattc aaccagtgca acaccaccag aggcaacgag gtgatcagcg tgatgaacag
     7381 agccaagaag gccggcaaga gcgtgggcgt ggtgaccacc accagagtgc agcacgccag
     7441 ccccgccggc acctacgccc acaccgtgaa cagaaactgg tacagcgacg ccgacgtgcc
     7501 cgccagcgcc agacaggagg gctgccagga catcgccacc cagctgatca gcaacatgga
     7561 catcgacgtg atcctgggcg gcggcagaaa gtacatgttc agaatgggca cccccgaccc
     7621 cgagtacccc gacgactaca gccagggcgg caccagactg gacggcaaga acctggtgca
     7681 ggagtggctg gccaagagac agggcgccag atacgtgtgg aacagaaccg agctgatgca
     7741 ggccagcctg gaccccagcg tgacccacct gatgggcctg ttcgagcccg gcgacatgaa
     7801 gtacgagatc cacagagaca gcaccctgga ccccagcctg atggagatga ccgaggccgc
     7861 cctgagactg ctgagcagaa accccagagg cttcttcctg ttcgtggagg gcggcagaat
     7921 cgaccacggc caccacgaga gcagagccta cagagccctg accgagacca tcatgttcga
     7981 cgacgccatc gagagagccg gccagctgac cagcgaggag gacaccctga gcctggtgac
     8041 cgccgaccac agccacgtgt tcagcttcgg cggctacccc ctgagaggca gcagcatctt
     8101 cggcctggcc cccggcaagg ccagagacag aaaggcctac accgtgctgc tgtacggcaa
     8161 cggccccggc tacgtgctga aggacggcgc cagacccgac gtgaccgaga gcgagagcgg
     8221 cagccccgag tacagacagc agagcgccgt gcccctggac gaggagaccc acgccggcga
     8281 ggacgtggcc gtgttcgcca gaggccccca ggcccacctg gtgcacggcg tgcaggagca
     8341 gaccttcatc gcccacgtga tggccttcgc cgcctgcctg gagccctaca ccgcctgcga
     8401 cctggccccc cccgccggca ccaccgacgc cgcccacccc ggctacagca gagtgggcgc
     8461 cgccggcaga ttcgagcaga ccgctagtgg tggttctggt ggaagcggag ctactaactt
     8521 cagcctgctg aagcaggctg gtgacgtgga ggagaaccct ggacctgcta gtgcgcgtaa
     8581 ggtcgatctc acctcctgcg atcgcgagcc gatccacatc cccggcagca ttcagccgtg
     8641 cggctgcctg cttgcctgcg acgcgcaggc ggtgcggatc acgcgcatta cggaaaatgc
     8701 cggcgcgttc tttggacgcg aaactccgcg ggtcggtgag ctactcgccg attacttcgg
     8761 cgagaccgaa gcccatgcgc tgcgcaacgc actggcgcag tcctccgatc caaagcgacc
     8821 ggcgctgatc ttcggttggc gcgacggcct gaccggccgc accttcgaca tctcactgca
     8881 tcgccatgac ggtacatcga tcatcgagtt cgagcctgcg gcggccgaac aggccgacaa
     8941 tccgctgcgg ctgacgcggc agatcatcgc gcgcaccaaa gaactgaagt cgctcgaaga
     9001 gatggccgca cgggtgccgc gctatctgca ggcgatgctc ggctatcacc gcgtgatgtt
     9061 gtaccgcttc gcggacgacg gctccgggat ggtgatcggc gaggcgaagc gcagcgacct
     9121 cgagagcttt ctcggtcagc actttccggc gtcgctggtc ccgcagcagg cgcggctact
     9181 gtacttgaag aacgcgatcc gcgtggtctc ggattcgcgc ggcatcagca gccggatcgt
     9241 gcccgagcac gacgcctccg gcgccgcgct cgatctgtcg ttcgcgcacc tgcgcagcat
     9301 ctcgccctgc catctcgaat ttctgcggaa catgggcgtc agcgcctcga tgtcgctgtc
     9361 gatcatcatt gacggcacgc tatggggatt gatcatctgt catcattacg agccgcgtgc
     9421 cgtgccgatg gcgcagcgcg tcgcggccga aatgttcgcc gacttcttat cgctgcactt
     9481 caccgccgcc caccaccaac gcgctagcgg atccaccggt gtctagaaag ctttgaggcc
     9541 ggcctgcagg cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     9601 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     9661 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     9721 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     9781 gtgggctcta tggtagctta ggaggcggaa agaaccagcg cgtggtaccc tcgagctacc
     9841 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     9901 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
     9961 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
    10021 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
    10081 gtctcagtag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
    10141 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
    10201 gtgggtccgg ggg
//
