LOCUS       sequence-366880-        5776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Trim41-PA-1D4 under the mouse Clgn promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Oura S, Hino T, Satoh T, Noda T, Koyano T, Isotani A, Matsuyama M, 
            Akira S, Ishiguro KI, Ikawa M
  TITLE     Trim41 is required to regulate chromosome axis protein dynamics and 
            meiosis in male mice.
  JOURNAL   PLoS Genet. 2022 Jun 1;18(6):e1010241. doi: 
            10.1371/journal.pgen.1010241.
  PUBMED    35648791
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2022-06-1"; volume: "18(6):e1010241. doi"; pages: " 
            10.1371/journal.pgen.1010241"
COMMENT     Sequence Label: pClgn1.1-Trim41-PA-1D4
FEATURES             Location/Qualifiers
     source          1..5776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1896..1915)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1961..2016
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2015..2034)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2371..2390)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2372..2390)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2400..2417)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2400..2416)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2409..2431)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2558..3013
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2645..2664)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2855..2876
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3039..3143
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3144..3212,3213..4004)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3362..3381)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4175..4763
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4664..4683
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4917..4934
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5051..5072
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5087..5092,5093..5110,5111..5117)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5125..5141
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5130..5152
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5149..5165
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5149..5165
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5184..5204
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5186..5204
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      5600..5609
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagttggatc gggaggagga ggaggaggag gtcggagagg aggaagaagt ggaggctgtc
       61 ggggctggcg ggggctggga cacccctatg cgggaagaag attatgaggg tgacatggag
      121 gaggaggccg aggaggaaga ggaagtgttc tggagtagtg gtataggtgg gtctaactgg
      181 gacaacatgg actatgtgtg ggaggaggag gaggaggagg aggaggaaga actggactat
      241 tacttggggg atgtggcgga cctgagaggg gaagatgagg acgaggagga ggaagtgctg
      301 gaggaagacg aggaagagga gctagacccg atcacccaac tgccgccacc tccagcccct
      361 cggagatgct tcacttgtcc ccagtgtcga aaaagttttc ctcggcggag cttccgcccc
      421 aacctgcagt tggccaacat ggtccaggtg atccgtcaga tgcacccaac tcctgggcga
      481 gggagccgtg tgaatgaaca aggcatctgt ccgagacatc aagaagccct gaagctgttc
      541 tgtgaggtgg atgaagaagc catctgtgtg gtgtgccgcg aatccaggag ccacaaacag
      601 catagtgtgg tgccattgga ggaggtagtg caggagtaca aggccaagct acaagggcat
      661 gtggaaccac ttaggaagca tctagaggct gtgcagaaga tgaaggccaa ggaggagagg
      721 cgggtgacag agctgaagag ccagatgaag tcagagctgg cagctgtggc ctcagagttt
      781 gggcggctaa caaggtttct ggctgaagag caggcaggat tagagaggcg cctccgggag
      841 atgcacgaag ctcagttagg acgtgcggga gcagcagcca accgccttga agagcaggct
      901 gcccagctga gccgcttgct tgctgaagcc caagaacgga gccaacaggg gggcctgcgg
      961 ctactgcaag acatcaagga gactttcaat aggtgtgaag agatacagtt gcagccccca
     1021 gagatttggt cccctgaccc atgccaaccc catagccacg acttcctgac agacgccatc
     1081 gtgaggaaaa tgagccgcat gttttgtcag gctgcaagag tggacctgac actggaccct
     1141 gacacagctc acccagctct gctgctgtcc cctgatcgcc gtggagtccg cttggcagaa
     1201 cgtcggcagg aggttcctga acattccaag cgcttctcag ctgactgctg tgtactaggg
     1261 gcccagggct tccgctctgg ccggcactac tgggaggtag aggtgggtgg gaggcgaggc
     1321 tgggcagtgg gtgctgcccg tgaatcaacc catcataaag aaaaagtggg ctctgggggg
     1381 tcctctgtta gcagtgggga tgccagctcc tcacgccatc atcatcgccg ccgccggctc
     1441 catctgcctc agcagctcct actccagcgc gaagtgtggt gtgtgggcac ccacggcaaa
     1501 cgataccagg cacagagctc aacagagcag acactgctca gcccatgtga gaaaccacgg
     1561 cgctttggcg tatacctgga ctatgaggct gggcgcctag gcttctacaa tgcggatacc
     1621 ttagcccatg tgcacacctt ctcagctgcc ttccttggcg agcgtgtttt cccttttttc
     1681 cgggtacttt ccaagggtac ccgaatcaag ctctgccctg aattcgatat cggcgttgcc
     1741 atgccaggtg ccgaagatga tgtggtgatc gggggcgggg gcacagagac cagccaagtg
     1801 gcgcctgcct agctcgagac tcctcaggtg caggctgcct atcagaaggt ggtggctggt
     1861 gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat
     1921 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
     1981 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
     2041 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
     2101 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct
     2161 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     2221 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     2281 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatgaaga
     2341 tccctcgact taattaaggt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     2401 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     2461 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     2521 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     2581 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     2641 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     2701 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     2761 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     2821 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     2881 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     2941 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     3001 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     3061 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     3121 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     3181 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     3241 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     3301 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     3361 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     3421 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     3481 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     3541 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     3601 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     3661 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     3721 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     3781 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     3841 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     3901 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     3961 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     4021 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     4081 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     4141 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     4201 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     4261 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     4321 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     4381 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     4441 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     4501 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     4561 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     4621 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     4681 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     4741 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     4801 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     4861 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     4921 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     4981 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     5041 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     5101 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     5161 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     5221 tcatcgatga gctccagaga gggactgacg ttggaaggac cgcatccccc caggcgcgcg
     5281 gccgctgggt cccgagcggc ggttgagcgg gccgcttgcg cactggggct gccactgcat
     5341 cctgcatcct ccggcgcggc cgctcgcgca cgcgcagtta gcagtcgctg ctgggcggct
     5401 gccggcggga tcgctctggg gagacgccgg gcaccaagta gcggccagtc ggacacctga
     5461 gctgccgctg ctggacacaa gcccgcagga caggtgtgta gaccgcgggc cgccttcctg
     5521 cggcttgttc tctggggacc gggtgagaaa tcggatctct agaggatccc tgcaggaatt
     5581 cgatatcaag cttgtcgacg ccaccatggc tgccgttgcc atgacaccca accctgtgca
     5641 gacccttcag gaggaggcgg tgtgtgccat ctgcctcgat tacttcacgg acccggtgtc
     5701 catcggctgc gggcacaact tctgccgagt gtgtgtaacc cagttgtggg gcggggagga
     5761 tgaggaagac agagat
//
