LOCUS       sequence-366899-       11205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Conditionally express both AsCas12a and CRISPR arrays on single 
            transcripts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11205)
  AUTHORS   Campa CC, Weisbach NR, Santinha AJ, Incarnato D, Platt RJ
  TITLE     Multiplexed genome engineering by Cas12a and CRISPR arrays encoded 
            on single transcripts.
  JOURNAL   Nat Methods. 2019 Aug 12. pii: 10.1038/s41592-019-0508-6. doi: 
            10.1038/s41592-019-0508-6.
  PUBMED    31406383
REFERENCE   2  (bases 1 to 11205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2019 Aug 12. pii: 10.1038/s41592-019-0508-6. doi: 
            10.1038/s41592-019-0508-6."
COMMENT     Sequence Label: pCI108-SiT-Cas12a-[Cond]
FEATURES             Location/Qualifiers
     source          1..11205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1210..1243
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    1410..1544
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    1664..1798
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    1918..2052
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    2081..2114
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2147..6067
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      5787..5796
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6068..6115
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6122..6202
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(6505..6522)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6511..6735
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6781..7209
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6868..6887)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7078..7099
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7218..7238)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7223..7552
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7403..7538
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7465..7484
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7619..8413
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7673..7692)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8283..8302
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8587..8708
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8757..8773)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8757..8773)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8770..8792)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8781..8797
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8805..8811,8812..8829,8830..8835))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8850..8871
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8988..9005)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9159..9747)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9239..9258)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9918..10709,10710..10778))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10541..10560
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10779..10883)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10958..10977)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag ctagccgtcg acatttaaat
     1201 catttaaata taacttcgta taatgtatgc tatacgaagt tattcgcgat gaataaatga
     1261 aagcttgcag atctgcgact ctagaggatc tgcgactcta gaggatcata atcagccata
     1321 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     1381 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     1441 aataaagcaa tagcatcaca aatttcacaa ataaagtatt tttttcactg cattctagtt
     1501 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgcgac tctagaggat
     1561 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     1621 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     1681 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     1741 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg
     1801 cgactctaga ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     1861 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     1921 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     1981 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     2041 catgtctgga tccccatcaa gctgatccgg aacccttaat ataacttcgt ataatgtatg
     2101 ctatacgaag ttattaggtc cctcgacctg cagcggtacc gccaccatga cacagttcga
     2161 gggctttacc aacctgtatc aggtgagcaa gacactgcgg tttgagctga tcccacaggg
     2221 caagaccctg aagcacatcc aggagcaggg cttcatcgag gaggacaagg cccgcaatga
     2281 tcactacaag gagctgaagc ccatcatcga tcggatctac aagacctatg ccgaccagtg
     2341 cctgcagctg gtgcagctgg attgggagaa cctgagcgcc gccatcgact cctatagaaa
     2401 ggagaaaacc gaggagacaa ggaacgccct gatcgaggag caggccacat atcgcaatgc
     2461 catccacgac tacttcatcg gccggacaga caacctgacc gatgccatca ataagagaca
     2521 cgccgagatc tacaagggcc tgttcaaggc cgagctgttt aatggcaagg tgctgaagca
     2581 gctgggcacc gtgaccacaa ccgagcacga gaacgccctg ctgcggagct tcgacaagtt
     2641 tacaacctac ttctccggct tttatgagaa caggaagaac gtgttcagcg ccgaggatat
     2701 cagcacagcc atcccacacc gcatcgtgca ggacaacttc cccaagttta aggagaattg
     2761 tcacatcttc acacgcctga tcaccgccgt gcccagcctg cgggagcact ttgagaacgt
     2821 gaagaaggcc atcggcatct tcgtgagcac ctccatcgag gaggtgtttt ccttcccttt
     2881 ttataaccag ctgctgacac agacccagat cgacctgtat aaccagctgc tgggaggaat
     2941 ctctcgggag gcaggcaccg agaagatcaa gggcctgaac gaggtgctga atctggccat
     3001 ccagaagaat gatgagacag cccacatcat cgcctccctg ccacacagat tcatccccct
     3061 gtttaagcag atcctgtccg ataggaacac cctgtctttc atcctggagg agtttaagag
     3121 cgacgaggaa gtgatccagt ccttctgcaa gtacaagaca ctgctgagaa acgagaacgt
     3181 gctggagaca gccgaggccc tgtttaacga gctgaacagc atcgacctga cacacatctt
     3241 catcagccac aagaagctgg agacaatcag cagcgccctg tgcgaccact gggatacact
     3301 gaggaatgcc ctgtatgagc ggagaatctc cgagctgaca ggcaagatca ccaagtctgc
     3361 caaggagaag gtgcagcgca gcctgaagca cgaggatatc aacctgcagg agatcatctc
     3421 tgccgcaggc aaggagctga gcgaggcctt caagcagaaa accagcgaga tcctgtccca
     3481 cgcacacgcc gccctggatc agccactgcc tacaaccctg aagaagcagg aggagaagga
     3541 gatcctgaag tctcagctgg acagcctgct gggcctgtac cacctgctgg actggtttgc
     3601 cgtggatgag tccaacgagg tggaccccga gttctctgcc cggctgaccg gcatcaagct
     3661 ggagatggag ccttctctga gcttctacaa caaggccaga aattatgcca ccaagaagcc
     3721 ctactccgtg gagaagttca agctgaactt tcagatgcct acactggcct ctggctggga
     3781 cgtgaataag gagaagaaca atggcgccat cctgtttgtg aagaacggcc tgtactatct
     3841 gggcatcatg ccaaagcaga agggcaggta taaggccctg agcttcgagc ccacagagaa
     3901 aaccagcgag ggctttgata agatgtacta tgactacttc cctgatgccg ccaagatgat
     3961 cccaaagtgc agcacccagc tgaaggccgt gacagcccac tttcagaccc acacaacccc
     4021 catcctgctg tccaacaatt tcatcgagcc tctggagatc acaaaggaga tctacgacct
     4081 gaacaatcct gagaaggagc caaagaagtt tcagacagcc tacgccaaga aaaccggcga
     4141 ccagaagggc tacagagagg ccctgtgcaa gtggatcgac ttcacaaggg attttctgtc
     4201 caagtatacc aagacaacct ctatcgatct gtctagcctg cggccatcct ctcagtataa
     4261 ggacctgggc gagtactatg ccgagctgaa tcccctgctg taccacatca gcttccagag
     4321 aatcgccgag aaggagatca tggatgccgt ggagacaggc aagctgtacc tgttccagat
     4381 ctataacaag gactttgcca agggccacca cggcaagcct aatctgcaca cactgtattg
     4441 gaccggcctg ttttctccag agaacctggc caagacaagc atcaagctga atggccaggc
     4501 cgagctgttc taccgcccta agtccaggat gaagaggatg gcacaccggc tgggagagaa
     4561 gatgctgaac aagaagctga aggatcagaa aaccccaatc cccgacaccc tgtaccagga
     4621 gctgtacgac tatgtgaatc acagactgtc ccacgacctg tctgatgagg ccagggccct
     4681 gctgcccaac gtgatcacca aggaggtgtc tcacgagatc atcaaggata ggcgctttac
     4741 cagcgacaag ttctttttcc acgtgcctat cacactgaac tatcaggccg ccaattcccc
     4801 atctaagttc aaccagaggg tgaatgccta cctgaaggag caccccgaga cacctatcat
     4861 cggcatcgat cggggcgaga gaaacctgat ctatatcaca gtgatcgact ccaccggcaa
     4921 gatcctggag cagcggagcc tgaacaccat ccagcagttt gattaccaga agaagctgga
     4981 caacagggag aaggagaggg tggcagcaag gcaggcctgg tctgtggtgg gcacaatcaa
     5041 ggatctgaag cagggctatc tgagccaggt catccacgag atcgtggacc tgatgatcca
     5101 ctaccaggcc gtggtggtgc tggagaacct gaatttcggc tttaagagca agaggaccgg
     5161 catcgccgag aaggccgtgt accagcagtt cgagaagatg ctgatcgata agctgaattg
     5221 cctggtgctg aaggactatc cagcagagaa agtgggaggc gtgctgaacc cataccagct
     5281 gacagaccag ttcacctcct ttgccaagat gggcacccag tctggcttcc tgttttacgt
     5341 gcctgcccca tatacatcta agatcgatcc cctgaccggc ttcgtggacc ccttcgtgtg
     5401 gaaaaccatc aagaatcacg agagccgcaa gcacttcctg gagggcttcg actttctgca
     5461 ctacgacgtg aaaaccggcg acttcatcct gcactttaag atgaacagaa atctgtcctt
     5521 ccagaggggc ctgcccggct ttatgcctgc atgggatatc gtgttcgaga agaacgagac
     5581 acagtttgac gccaagggca cccctttcat cgccggcaag agaatcgtgc cagtgatcga
     5641 gaatcacaga ttcaccggca gataccggga cctgtatcct gccaacgagc tgatcgccct
     5701 gctggaggag aagggcatcg tgttcaggga tggctccaac atcctgccaa agctgctgga
     5761 gaatgacgat tctcacgcca tcgacaccat ggtggccctg atccgcagcg tgctgcagat
     5821 gcggaactcc aatgccgcca caggcgagga ctatatcaac agccccgtgc gcgatctgaa
     5881 tggcgtgtgc ttcgactccc ggtttcagaa cccagagtgg cccatggacg ccgatgccaa
     5941 tggcgcctac cacatcgccc tgaagggcca gctgctgctg aatcacctga aggagagcaa
     6001 ggatctgaag ctgcagaacg gcatctccaa tcaggactgg ctggcctaca tccaggagct
     6061 gcgcaacaaa aggccggcgg ccacgaaaaa ggccggccag gcaaaaaaga aaaagggatc
     6121 ctacccatac gatgttccag attacgctta tccctacgac gtgcctgatt atgcataccc
     6181 atatgatgtc cccgactatg cctaagaatt cgattcgtca gtagggttgt aaaggttttt
     6241 cttttcctga gaaaacaacc ttttgttttc tcaggttttg ctttttggcc tttccctagc
     6301 tttaaaaaaa aaaaagcaaa actcaccgag gcagttccat aggatggcaa gatcctggta
     6361 ttggtctgcg aaatttctac tcttgtagat ggagacgcaa attctaatcc agcttcaaca
     6421 gctatttcac aagctttcat gatttctcgt ctctaatttc tactcttgta gattctagag
     6481 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     6541 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     6601 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     6661 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     6721 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     6781 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6841 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6901 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6961 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     7021 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7081 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     7141 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     7201 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     7261 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     7321 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     7381 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7441 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     7501 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     7561 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
     7621 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     7681 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     7741 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     7801 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     7861 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     7921 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     7981 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     8041 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     8101 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     8161 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     8221 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     8281 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     8341 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     8401 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     8461 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     8521 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     8581 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8641 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8701 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     8761 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     8821 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8881 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8941 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     9001 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     9061 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9121 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9181 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9241 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9301 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9361 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9421 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9481 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9541 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     9601 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9661 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9721 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9781 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9841 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9901 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9961 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    10021 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10081 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10141 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10201 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10261 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10321 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10381 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10441 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10501 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10561 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10621 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10681 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10741 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10801 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10861 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
    10921 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
    10981 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
    11041 aaatttaagc tacaacaagg caaggcttga ccgacttcgc gttgacattg attattgact
    11101 agtcttaatt aacgcgttta attaatgcaa agatggataa agttttaaac agagaggaat
    11161 ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaatt
//
