LOCUS       sequence-36690-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  TITLE     C-terminal fluorescent fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET mCerulean LIC cloning vector (H6-mCerulean)
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(53..73)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(74..91)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(92..136)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     RBS             149..171
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    186..210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(210..229)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(211..229)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(313..332)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        538..615
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(635..654)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 tctcgccctt gctcacggat ccgctgctgc cgttgctccc actaccaata ttggattgga
       61 agtacaggtt ttcatggtga tggtgatggt gttcatgcca ttcaattttc tgcgcttcaa
      121 aaatatcgtt caggcctccc atggtatatc tccttcttaa agttaaacaa aattatttct
      181 agaggggaat tgttatccgc tcacaattcc cctatagtga gtcgtattaa tttcgcggga
      241 tcgagatctc gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
      301 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
      361 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
      421 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
      481 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga gatcccggac
      541 accatcgaat ggcgcaaaac ctttcgcggt atggcatgat agcgcccgga agagagtcaa
      601 ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc
      661 tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg
      721 gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa
      781 ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg
      841 ccgtcgcaaa ttgtcgcggc gattaaaatc tcgcgccgat caactggg
//
