LOCUS       sequence-366904-       12890 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express GFP and MAN1C1 proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12890)
  AUTHORS   Ladaigue S, Lefranc AC, Balde K, Quitoco M, Bacquer E, Busso D, 
            Piton G, Depagne J, Dechamps N, Yamakawa N, Debusschere L, Han C, 
            Allain F, Buard V, Tarlet G, Francois A, Paget V, Milliat F, Guipaud
            O
  TITLE     A role for endothelial alpha-mannosidase MAN1C1 in radiation-induced
            immune cell recruitment.
  JOURNAL   iScience. 2022 Nov 2;25(12):105482. doi: 10.1016/j.isci.2022.105482.
            eCollection 2022 Dec 22.
  PUBMED    36404925
REFERENCE   2  (bases 1 to 12890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-11-2"; volume: "25(12):105482. doi"; pages: " 
            10.1016/j.isci.2022.105482. eCollection 2022 Dec 22"
COMMENT     Sequence Label: pTRIP-MND-HsMAN1C1-1-630-cMycFlag-hPGK-GFP
FEATURES             Location/Qualifiers
     source          1..12890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2054..2171
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             4690..4719
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4738..4761
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        4779..5289
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5208..5226
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      5299..5308
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5305..5307,5308..5310,5311..6024)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5305..5307,5308..5310,5311..6021)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5350..5371)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5611..5630)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5958..5979
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6310..6490
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8018..8037)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8251..8270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8370..8392
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8430..8448)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8516..8620
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8621..8689,8690..9481)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8839..8858)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9652..10240
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10141..10160
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10394..10411
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10481..10501)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10486..10815
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10666..10801
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11949..12014
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12144..12164
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12589..12723
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12626..12645)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12680..12699
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gccgaattca caaatggcag
     2041 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2101 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2161 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggctg atacgcgtca
     2221 tcgatcgatt agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga
     2281 ctcaacaata tcaccagctg aagcctatag agtacgagcc atagatagaa taaaagattt
     2341 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
     2401 gatcaaggtt aggaacagag agacagcaga atatgggcca aacaggatat ctgtggtaag
     2461 cagttcctgc cccggctcag ggccaagaac agttggaaca gcagaatatg ggccaaacag
     2521 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat
     2581 gcggtcccgc cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga
     2641 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc
     2701 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggcgcgatct
     2761 ggatctgccg ccgcgatcgc catgctcatg aggaaagtgc ccggcttcgt cccggcctcc
     2821 ccgtgggggc tgcggctgcc gcagaagttc ctcttcctcc tcttcctctc gggcctggtc
     2881 accctgtgct tcggggccct cttcctgctg ccccactcct ctcgcctcaa gcgcctcttc
     2941 ctggcccccc ggacccagca gcctggtctg gaagtggtgg ctgaaatcgc cggccatgcc
     3001 ccggcccgcg agcaggagcc gcctcccaac ccggcccccg ccgcgccggc cccgggcgag
     3061 gatgacccca gcagctgggc cagtccccgc cgcaggaaag gggggctgcg gcgcacccgc
     3121 cccactggac cccgcgagga ggccacggcg gcccggggca atagcatccc ggcctccagg
     3181 cccggggacg agggcgtccc tttccgcttt gacttcaacg cattccggag ccgtctccgc
     3241 cacccggtcc tgggaacgag ggccgatgag agtcaggagc cccagagcca agtgcgagcc
     3301 cagcgggaga aaatcaagga gatgatgcag tttgcttggc agagctataa gcgttatgca
     3361 atggggaaaa acgaactccg tccactaaca aaagatggct acgagggtaa catgttcgga
     3421 ggcctcagcg gggcaacagt cattgactcc ctcgataccc tctacctcat ggagctgaag
     3481 gaggagttcc aggaggccaa ggcctgggtg ggagagagct tccacctgaa cgtgagcgga
     3541 gaagcatcct tgtttgaggt gaacatccgc tacatcgggg gactcctctc agccttctac
     3601 ctgacaggag aagaggtgtt ccgaataaag gccatcaggc tgggagagaa gctcctgccg
     3661 gcgttcaaca cccccacggg aatcccaaag ggcgtggtga gcttcaaaag tgggaactgg
     3721 ggctgggcca cagccggcag cagcagcatc ttggcggagt ttggatccct gcacttggaa
     3781 ttcttacacc tcactgaact ctctggcaac caggtcttcg ctgaaaaggt caggaacatc
     3841 cgcaaggtcc tcaggaagat cgaaaagccc tttggcctct accccaactt cctcagccca
     3901 gtgagtggga actgggtgca acaccatgtc tcagttggag gactcgggga cagtttttat
     3961 gaatatttga tcaaatcctg gttgatgtcg ggcaagacag atatggaggc taaaaatatg
     4021 tactacgaag ccttggaggc gatagagacc tacttgctga atgtctctcc cggggggctg
     4081 acctacattg ccgagtggcg aggggggatt ctggaccaca agatggggca cctggcctgt
     4141 ttctccgggg gcatgatcgc ccttggcgcc gaggatgcca aggaagaaaa gagggcccac
     4201 taccgagagc tcgcagccca gatcaccaag acgtgtcacg agtcatacgc ccgctcagac
     4261 accaaacttg ggcctgaggc cttctggttt aactccggca gagaggccgt ggccacccag
     4321 ctgagcgaga gctactacat cctccggcca gaggtggtgg agagctacat gtacctgtgg
     4381 cgacagaccc acaaccccat ctacagggag tggggctggg aggtggtgct ggccttggag
     4441 aaatactgtc ggacagaagc cggtttctct gggatccaag acgtgtacag tagcaccccc
     4501 aaccacgaca acaagcagca gagcttcttt ctagcggaga cactaaagta tctctatctt
     4561 ctgttctctg aagatgactt gctctccctg gaagactggg tgttcaacac cgaggcccac
     4621 ccactcccgg tgaaccactc agacagctcc ggcagagcct ggggcagaca cacgcgtacg
     4681 cggccgctcg agcagaaact catctcagaa gaggatctgg cagcaaatga tatcctggat
     4741 tacaaggatg acgacgataa ggtttaacga attcccacgg ggttggggtt gcgccttttc
     4801 caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc
     4861 agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc
     4921 ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa
     4981 ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt
     5041 accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg
     5101 gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg
     5161 gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc
     5221 tgcaagcctc cggagcgcgc gtcggcagtc ggctccctcg ttgaccgaat caccgacctc
     5281 tctccccagc caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
     5341 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     5401 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     5461 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     5521 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     5581 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     5641 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     5701 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     5761 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     5821 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     5881 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     5941 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     6001 ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gctcgagacc tagaaaaaca
     6061 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     6121 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     6181 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6241 aattcactcc caacgaagac aaaatcgtcg agagatgctg catataagca gctgcttttt
     6301 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     6361 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     6421 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     6481 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     6541 tgaatatcag agagtgagag gccttgacat tataatagat ttagcaggaa ttgaactagg
     6601 agtggagcac acaggcaaag ctgcagaagt acttggaaga agccaccaga gatactcacg
     6661 attctgcaca tacctggcta atcccagatc ctaaggatta cattaagttt actaacattt
     6721 atataatgat ttatagttta aagtataaac ttatctaatt tactattctg acagatatta
     6781 attaatcctc aaatatcata agagatgatt actattatcc ccatttaaca caagaggaaa
     6841 ctgagaggga aagatgttga agtaattttc ccacaattac agcatccgtt agttacgact
     6901 ctatgatctt ctgacacaaa ttccatttac tcctcaccct atgactcagt cgaatatatc
     6961 aaagttatgg acattatgct aagtaacaaa ttaccctttt atatagtaaa tactgagtag
     7021 attgagagaa gaaattgttt gcaaacctga atagcttcaa gaagaagaga agtgaggata
     7081 agaataacag ttgtcattta acaagtttta acaagtaact tggttagaaa gggattcaaa
     7141 tgcataaagc aagggataaa tttttctggc aacaagacta tacaatataa ccttaaatat
     7201 gacttcaaat aattgttgga acttgataaa actaattaaa tattattgaa gattatcaat
     7261 attataaatg taatttactt ttaaaaaggg aacatagaaa tgtgtatcat tagagtagaa
     7321 aacaatcctt attatcacaa tttgtcaaaa caagtttgtt attaacacaa gtagaatact
     7381 gcattcaatt aagttgactg cagattttgt gttttgttaa aattagaaag agataacaac
     7441 aatttgaatt attgaaagta acatgtaaat agttctacat acgttctttt gacatcttgt
     7501 tcaatcattg atcgaagttc tttatcttgg aagaatttgt tccaaagact ctgaaataag
     7561 gaaaacaatc tattatatag tctcacacct ttgttttact tttagtgatt tcaatttaat
     7621 aatgtaaatg gttaaaattt attcttctct gagatcattt cacattgcag atagaaaacc
     7681 tgagactggg gtaattttta ttaaaatcta atttaatctc agaaacacat ctttattcta
     7741 acatcaattt ttccagtttg atattatcat ataaagtcag ccttcctcat ctgcaggttc
     7801 cacaacaaaa atccaaccaa ctgtggatca aaaatattgg gaaaaaatta aaaatagcaa
     7861 tacaacaata aaaaaataca aatcagaaaa acagcacagt ataacaactt tatttagcat
     7921 ttacaatcta ttaggtatta taagtaatct agccagatcc tctacgccgg acgcatcgtg
     7981 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
     8041 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
     8101 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
     8161 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
     8221 aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     8281 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     8341 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     8401 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     8461 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     8521 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     8581 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     8641 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     8701 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     8761 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     8821 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     8881 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     8941 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     9001 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     9061 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     9121 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     9181 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     9241 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     9301 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     9361 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     9421 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     9481 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     9541 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     9601 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     9661 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     9721 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     9781 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     9841 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     9901 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     9961 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    10021 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    10081 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    10141 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    10201 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    10261 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    10321 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
    10381 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
    10441 gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg
    10501 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
    10561 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
    10621 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
    10681 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
    10741 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
    10801 ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc
    10861 actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact
    10921 ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt
    10981 ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg
    11041 ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg
    11101 gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa
    11161 ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc
    11221 gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac
    11281 ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt
    11341 gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc
    11401 ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc
    11461 tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg
    11521 cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct
    11581 gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg
    11641 ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca
    11701 ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat
    11761 cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata
    11821 cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg
    11881 tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa
    11941 gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
    12001 tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct
    12061 ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg
    12121 ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact
    12181 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt
    12241 gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa
    12301 aatattctgt aacctttata agtaggcata acagttataa tcataacata ctgttttttc
    12361 ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct
    12421 ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta
    12481 gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
    12541 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
    12601 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    12661 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
    12721 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt
    12781 accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt
    12841 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
