LOCUS       sequence-366906-        7875 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pFastBac1 (baculovirus polyhedrin promoter) encoding HA signal 
            peptide, TEV-protease cleavable His10-tag, and full-length human 
            pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Zhang S, Hansen DT, Martin-Garcia JM, Zook JD, Pan S, Craciunescu 
            FM, Burnett JC Jr, Fromme P
  TITLE     Purification, characterization, and preliminary serial 
            crystallography diffraction advances structure determination of 
            full-length human particulate guanylyl cyclase A receptor.
  JOURNAL   Sci Rep. 2022 Jul 12;12(1):11824. doi: 10.1038/s41598-022-15798-z.
  PUBMED    35821229
REFERENCE   2  (bases 1 to 7875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-12"; volume: "12(1):11824. doi"; pages: " 
            10.1038/s41598-022-15798-z"
COMMENT     Sequence Label: pFastBac1-pGC-A
FEATURES             Location/Qualifiers
     source          1..7875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(89..108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        482..586
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(587..655,656..1447)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(805..824)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1618..2206
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2107..2126
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2360..2377
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2511..2735
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2802..3335)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3524..3529,3530..3546,3547..3552))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3904..3995
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3920..3937
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4086..4115
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             4125..4145
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    7365..7499
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7402..7421)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7456..7475
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  7528..7693
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt cttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
      481 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      541 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      601 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      661 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      721 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
      781 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
      841 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
      901 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
      961 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     1021 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     1081 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     1141 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     1201 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     1261 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     1321 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     1381 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     1441 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     1501 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1561 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1621 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1681 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1741 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     1801 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1861 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     1921 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     1981 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2041 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2101 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2161 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2221 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2281 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     2341 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     2401 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct
     2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc
     3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
     3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
     3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
     3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
     3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
     4021 ccatcgggcg cggatccatg aaaactatta tcgccctgtc ttacatcttc tgtctcgtct
     4081 tcgcacatca ccaccaccac catcaccacc atcacggcgc gccggaaaac ctgtacttcc
     4141 agggtggcaa cctcactgtg gctgtggtcc tgccactggc taacacttcc tacccttggt
     4201 cttgggctcg tgtgggtcca gctgtcgagc tggccctggc tcaggtcaag gccagacctg
     4261 acctgctgcc cggctggacc gtgcgcactg tcctgggttc cagcgagaac gctctgggtg
     4321 tgtgctctga cactgctgct cctctggctg ctgtggacct gaagtgggaa cacaacccag
     4381 ccgtgttcct gggacctggt tgcgtgtacg ctgccgctcc agtcggaaga ttcaccgctc
     4441 actggcgcgt ccctctgctc actgctggtg ctcccgctct gggattcgga gtgaaggacg
     4501 agtacgctct gaccactcgt gccggaccta gctacgctaa gctgggtgac ttcgtggctg
     4561 ctctgcaccg ccgtctgggt tgggagaggc aggccctgat gctgtacgct tacaggcccg
     4621 gagacgagga acactgcttc ttcctggtgg aaggtctgtt catgcgcgtc cgtgacaggc
     4681 tgaacatcac tgtggaccac ctggagttcg ccgaagacga cctgtctcac tacacccgcc
     4741 tgctgcgtac tatgcccagg aagggcagag tgatctacat ctgctcttca ccagacgctt
     4801 tccgcactct catgctcctg gctctggagg ctggcctgtg cggagaagac tacgtcttct
     4861 tccacctgga catcttcggt cagagcctgc agggaggtca aggaccagct cctcgccgcc
     4921 cttgggagag aggtgacgga caggacgtgt ctgctcgcca ggccttccag gctgctaaga
     4981 tcatcaccta caaggacccc gacaacccag agtacctgga gttcctgaag cagctgaagc
     5041 acctggccta cgagcagttc aacttcacta tggaggacgg cctggtcaac actatccccg
     5101 ctagcttcca cgacggactg ctgctgtaca tccaagctgt cactgagact ctggctcacg
     5161 gcggaaccgt gactgacggt gaaaacatca ctcagaggat gtggaacaga tcattccagg
     5221 gtgtgaccgg ctacctgaag atcgactcca gcggcgaccg tgagactgac ttctccctgt
     5281 gggacatgga cccagaaaac ggcgccttca gggtggtcct gaactacaac ggaaccagcc
     5341 aggagctggt ggctgtctct ggaaggaagc tgaactggcc actgggttac cctcccccag
     5401 acatccctaa gtgcggtttc gacaacgagg accccgcctg caaccaggac cacctgtcca
     5461 ctctggaagt gctggctctg gtcggatctc tgtcactgct gggtatcctg atcgtctcat
     5521 tcttcatcta ccgcaagatg cagctggaga aggaactggc ctccgagctg tggagagtgc
     5581 gctgggagga cgtcgaacca tcttcactgg aacgccacct gcgttccgct ggaagccgtc
     5641 tgaccctgtc tggaaggggt tcaaactacg gctccctgct gaccactgag ggacagttcc
     5701 aggtgttcgc taagactgcc tactacaagg gtaacctggt ggccgtcaag cgtgtcaacc
     5761 gtaagaggat cgagctgacc aggaaggtgc tgttcgaact gaagcacatg cgtgacgtcc
     5821 agaacgagca cctgaccagg ttcgtgggcg cttgcactga ccctcccaac atctgcatcc
     5881 tgactgaata ctgccctaga ggatcactgc aggacatcct ggagaacgaa tccatcaccc
     5941 tggactggat gttccgctac agcctgacta acgacatcgt gaagggcatg ctgttcctgc
     6001 acaacggcgc tatctgctct cacggaaacc tgaagtccag caactgcgtg gtggacggta
     6061 gattcgtcct gaagatcacc gactacggcc tggagtcatt ccgcgacctg gaccctgaac
     6121 agggccacac cgtgtacgcc aagaagctgt ggactgctcc cgaactgctg agaatggcta
     6181 gcccacctgt gcgcggctct caggctggag acgtctactc cttcggaatc atcctgcagg
     6241 agatcgctct gcgtagcggt gtgttccacg tcgaaggcct ggacctgtct ccaaaggaga
     6301 tcatcgaaag ggtgaccaga ggagagcagc ccccattcag gccttcactg gccctgcagt
     6361 cccacctgga ggaactgggt ctgctgatgc agagatgctg ggctgaggac cctcaggaac
     6421 gccctccctt ccagcagatc cgcctgactc tgcgtaagtt caacagggag aactcttcaa
     6481 acatcctgga caacctgctg agcagaatgg aacagtacgc caacaacctg gaggaactgg
     6541 tcgaggaacg tacccaggct tacctggagg aaaagaggaa ggctgaggcc ctgctgtacc
     6601 agatcctgcc ccactctgtg gctgaacagc tgaagcgcgg tgaaaccgtg caggctgaag
     6661 ccttcgactc agtcactatc tacttctccg acatcgtggg attcaccgct ctgtctgccg
     6721 agtcaacccc aatgcaggtg gtcactctgc tgaacgacct gtacacttgc ttcgacgccg
     6781 tcatcgacaa cttcgacgtg tacaaggtcg aaaccatcgg tgacgcttac atggtggtgt
     6841 ccggactgcc agtccgcaac ggtcgtctgc acgcttgcga agtggccaga atggctctgg
     6901 ctctgctgga cgccgtgcgc tccttcagaa tccgccaccg tcctcaggag cagctgaggc
     6961 tgagaatcgg tatccacact ggcccagtct gcgctggagt ggtcggtctg aagatgcctc
     7021 gttactgcct gttcggtgac accgtgaaca ctgccagcag gatggagtct aacggcgaag
     7081 ctctgaagat ccacctgtcc agcgaaacca aggctgtcct ggaggagttc ggtggcttcg
     7141 agctggaact gcgcggcgac gtggaaatga agggaaaagg caaagtcagg acatactggt
     7201 tgttgggaga aagaggtagc agcacccgtg gataaaagct tgtcgagaag tactagagga
     7261 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     7321 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     7381 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7441 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct
     7501 gatcactgct tgagcctagg agatccgaac cagataagtg aaatctagtt ccaaactatt
     7561 ttgtcatttt taattttcgt attagcttac gacgctacac ccagttccca tctattttgt
     7621 cactcttccc taaataatcc ttaaaaactc catttccacc cctcccagtt cccaactatt
     7681 ttgtccgccc acagcggggc atttttcttc ctgttatgtt tttaatcaaa catcctgcca
     7741 actccatgtg acaaaccgtc atcttcggct actttttctc tgtcacagaa tgaaaatttt
     7801 tctgtcatct cttcgttatt aatgtttgta attgactgaa tatcaacgct tatttgcagc
     7861 ctgaatggcg aatgg
//
