LOCUS       sequence-366953-       10020 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible overexpression of SMARCD3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10020)
  AUTHORS   Romero-Tejeda M, Fonoudi H, Weddle CJ, DeKeyser JM, Lenny B, 
            Fetterman KA, Magdy T, Sapkota Y, Epting C, Burridge PW
  TITLE     A novel transcription factor combination for direct reprogramming to
            a spontaneously contracting human cardiomyocyte-like state.
  JOURNAL   J Mol Cell Cardiol. 2023 Jul 6:S0022-2828(23)00106-2. doi: 
            10.1016/j.yjmcc.2023.06.005.
  PUBMED    37421991
REFERENCE   2  (bases 1 to 10020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Cell 
            Cardiol. 2023 Jul 6:S0022-2828(23)00106-2. doi: 
            10.1016/j.yjmcc.2023.06.005."
COMMENT     Sequence Label: tetO-SMARCD3
FEATURES             Location/Qualifiers
     source          1..10020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(49..68)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        240..619
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        621..819
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     774..794
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             837..1017
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1064..1189
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1682..1915
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2100..2144
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2442..2559
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2612..2630,2631..2653,2654..2672,2673..2695,2696..2714,
                     2715..2737,2738..2756,2757..2779,2780..2798,2799..2821,
                     2822..2840,2841..2863,2864..2882)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2915..2952
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2949..2973
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     3052..3071
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3052..3070
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3102..3126
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4546..4570)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4596..4637
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4647..4664
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    4714..5302
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4767..4787)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5185..5196)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5305..5321)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5306..5322)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5827..6007
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6033..6050)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6039..6263
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6309..6737
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6396..6415)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6606..6627
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6746..6766)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6751..7080
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6931..7066
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6993..7012
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7128..7175
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7194..7568
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7698..7819
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7735..7754)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7789..7808
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7868..7884)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7868..7884)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7881..7903)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7892..7908
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7916..7922,7923..7940,7941..7946))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7961..7982
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8099..8116)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8270..8858)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8350..8369)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9029..9820,9821..9889))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9652..9671
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9890..9994)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
       61 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
      121 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
      181 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
      241 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      301 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      361 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      421 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      481 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      541 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      601 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      661 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      721 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      781 gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt
      841 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
      901 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
      961 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     1021 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     1081 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     1141 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     1201 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     1261 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     1321 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     1381 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     1441 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     1501 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
     1561 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     1621 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     1681 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     1741 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     1801 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     1861 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     1921 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     1981 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     2041 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     2101 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     2161 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     2221 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     2281 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     2341 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc
     2401 gtgcgccaat tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
     2461 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     2521 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     2581 agagatccag tttggttatc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     2641 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     2701 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     2761 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     2821 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     2881 gaaaagtgaa agtcgagctc ggtacccggg tcgagtaggc gtgtacggtg ggaggcctat
     2941 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     3001 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt gtaatacgac
     3061 tcactatagg gagacccaag ctggctaggt aagcttgatc aacaagtttg tacaaaaaag
     3121 caggcttcac catgactcca ggtcttcagc acccacccac cgtggtacag cgccccggga
     3181 tgccgtctgg agcccggatg ccccaccagg gggcgcccat gggccccccg ggctccccgt
     3241 acatgggcag ccccgccgtg cgacccggcc tggcccccgc gggcatggag cccgcccgca
     3301 agcgagcagc gcccccgccc gggcagagcc aggcacagag ccagggccag ccggtgccca
     3361 ccgcccccgc gcggagccgc agtgccaaga ggaggaagat ggctgacaaa atcctccctc
     3421 aaaggattcg ggagctggtc cccgagtccc aggcttacat ggacctcttg gcatttgaga
     3481 ggaaactgga tcaaaccatc atgcggaagc gggtggacat ccaggaggct ctgaagaggc
     3541 ccatgaagca aaagcggaag ctgcgactct atatctccaa cacttttaac cctgcgaagc
     3601 ctgatgctga ggattccgac ggcagcattg cctcctggga gctacgggtg gaggggaagc
     3661 tcctggatga tcccagcaaa cagaagcgga agttctcttc tttcttcaag agtttggtca
     3721 tcgagctgga caaagatctt tatggccctg acaaccacct cgttgagtgg catcggacac
     3781 ccacgaccca ggagacggac ggcttccagg tgaaacggcc tggggacctg agtgtgcgct
     3841 gcacgctgct cctcatgctg gactaccagc ctccccagtt caaactggat ccccgcctag
     3901 cccggctgct ggggctgcac acacagagcc gctcagccat tgtccaggcc ctgtggcagt
     3961 atgtgaagac caacaggctg caggactccc atgacaagga atacatcaat ggggacaagt
     4021 atttccagca gatttttgat tgtccccggc tgaagttttc tgagattccc cagcgcctca
     4081 cagccctgct attgccccct gacccaattg tcatcaacca tgtcatcagc gtggaccctt
     4141 cagaccagaa gaagacggcg tgctatgaca ttgacgtgga ggtggaggag ccattaaagg
     4201 ggcagatgag cagcttcctc ctatccacgg ccaaccagca ggagatcagt gctctggaca
     4261 gtaagatcca tgagacgatt gagtccataa accagctcaa gatccagagg gacttcatgc
     4321 taagcttctc cagagacccc aaaggctatg tccaagacct gctccgctcc cagagccggg
     4381 acctcaaggt gatgacagat gtagccggca accctgaaga ggagcgccgg gctgagttct
     4441 accaccagcc ctggtcccag gaggccgtca gtcgctactt ctactgcaag atccagcagc
     4501 gcaggcagga gctggagcag tcgctggttg tgcgcaacac ctagaaccca gctttcttgt
     4561 acaaagtggt tgatctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc
     4621 tcggtctcga ttctacgcgt accggtcatc atcaccatca ccattgagtt taaacccgct
     4681 gatcagcctc aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     4741 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     4801 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     4861 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     4921 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     4981 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     5041 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     5101 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     5161 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     5221 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     5281 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga
     5341 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     5401 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     5461 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     5521 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     5581 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg
     5641 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac
     5701 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta
     5761 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac
     5821 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     5881 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     5941 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6001 tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     6061 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     6121 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     6181 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     6241 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     6301 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     6361 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     6421 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6481 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     6541 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6601 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     6661 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     6721 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     6781 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     6841 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     6901 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     6961 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     7021 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     7081 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat
     7141 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca
     7201 agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct
     7261 ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc
     7321 gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc
     7381 tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt
     7441 ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg
     7501 ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc
     7561 aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     7621 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     7681 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7741 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7801 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     7861 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7921 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     7981 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     8041 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     8101 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     8161 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     8221 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8281 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8341 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8401 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8461 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8521 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8581 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8641 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8701 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8761 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8821 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8881 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8941 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     9001 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     9061 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     9121 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     9181 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     9241 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     9301 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     9361 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9421 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9481 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9541 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9601 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9661 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9721 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9781 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9841 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9901 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9961 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
//
