LOCUS       sequence-366956-        7025 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7025)
  AUTHORS   Young-Pearse TL, Bai J, Chang R, Zheng JB, LoTurco JJ, Selkoe DJ
  TITLE     A critical function for beta-amyloid precursor protein in neuronal 
            migration revealed by in utero RNA interference.
  JOURNAL   J Neurosci. 2007 Dec 26. 27(52):14459-69.
  PUBMED    18160654
REFERENCE   2  (bases 1 to 7025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2007 Dec 26. 27(52):14459-69."
COMMENT     Sequence Label: pCAX FLAG APP
FEATURES             Location/Qualifiers
     source          1..7025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          657..1673
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1597..1619
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1681..1700
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1797..1820
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2097..2108)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     CDS             2588..2608
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     primer_bind     complement(4043..4062)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4108..4163
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4162..4181)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4524..4540)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4524..4540)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4537..4559)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4548..4564
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4572..4578,4579..4596,4597..4602))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4617..4638
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4697..4892
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4743..4878
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4898..5032
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4935..4954)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4989..5008
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5100..5117)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5271..5859)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5351..5370)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6030..6821,6822..6890))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6653..6672
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6891..6995)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg nnsgcgccrg gcggggsggg gsggggsgrg gggsggggsg
      541 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
      601 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
      661 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
      721 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      781 gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc
      841 ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt
      901 gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg
      961 ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg
     1021 gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc
     1141 ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg
     1201 cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc
     1261 cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct
     1441 ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc
     1501 ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg
     1561 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     1621 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg
     1681 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattcagg taccgatatc
     1741 gagccatgct gcccggtttg gcactgctcc tgctggccgc ctggacggct cgggcggatt
     1801 acaaggatga cgacgataag ctggaggtac ccactgatgg taatgctggc ctgctggctg
     1861 aaccccagat tgccatgttc tgtggcagac tgaacatgca catgaatgtc cagaatggga
     1921 agtgggattc agatccatca gggaccaaaa cctgcattga taccaaggaa ggcatcctgc
     1981 agtattgcca agaagtctac cctgaactgc agatcaccaa tgtggtagaa gccaaccaac
     2041 cagtgaccat ccagaactgg tgcaagcggg gccgcaagca gtgcaagacc catccccact
     2101 ttgtgattcc ctaccgctgc ttagttggtg agtttgtaag tgatgccctt ctcgttcctg
     2161 acaagtgcaa attcttacac caggagagga tggatgtttg cgaaactcat cttcactggc
     2221 acaccgtcgc caaagagaca tgcagtgaga agagtaccaa cttgcatgac tacggcatgt
     2281 tgctgccctg cggaattgac aagttccgag gggtagagtt tgtgtgttgc ccactggctg
     2341 aagaaagtga caatgtggat tctgctgatg cggaggagga tgactcggat gtctggtggg
     2401 gcggagcaga cacagactat gcagatggga gtgaagacaa agtagtagaa gtagcagagg
     2461 aggaagaagt ggctgaggtg gaagaagaag aagccgatga tgacgaggac gatgaggatg
     2521 gtgatgaggt agaggaagag gctgaggaac cctacgaaga agccacagag agaaccacca
     2581 gcattgccac caccaccacc accaccacag agtctgtgga agaggtggtt cgagaggtgt
     2641 gctctgaaca agccgagacg gggccgtgcc gagcaatgat ctcccgctgg tactttgatg
     2701 tgactgaagg gaagtgtgcc ccattctttt acggcggatg tggcggcaac cggaacaact
     2761 ttgacacaga agagtactgc atggccgtgt gtggcagcgc cattcctaca acagcagcca
     2821 gtacccctga tgccgttgac aagtatctcg agacacctgg ggatgagaat gaacatgccc
     2881 atttccagaa agccaaagag aggcttgagg ccaagcaccg agagagaatg tcccaggtca
     2941 tgagagaatg ggaagaggca gaacgtcaag caaagaactt gcctaaagct gataagaagg
     3001 cagttatcca gcatttccag gagaaagtgg aatctttgga acaggaagca gccaacgaga
     3061 gacagcagct ggtggagaca cacatggcca gagtggaagc catgctcaat gaccgccgcc
     3121 gcctggccct ggagaactac atcaccgctc tgcaggctgt tcctcctcgg cctcgtcacg
     3181 tgttcaatat gctaaagaag tatgtccgcg cagaacagaa ggacagacag cacaccctaa
     3241 agcatttcga gcatgtgcgc atggtggatc ccaagaaagc cgctcagatc cggtcccagg
     3301 ttatgacaca cctccgtgtg atttatgagc gcatgaatca gtctctctcc ctgctctaca
     3361 acgtgcctgc agtggccgag gagattcagg atgaagttga tgagctgctt cagaaagagc
     3421 aaaactattc agatgacgtc ttggccaaca tgattagtga accaaggatc agttacggaa
     3481 acgatgctct catgccatct ttgaccgaaa cgaaaaccac cgtggagctc cttcccgtga
     3541 atggagagtt cagcctggac gatctccagc cgtggcattc ttttggggct gactctgtgc
     3601 cagccaacac agaaaacgaa gttgagcctg ttgatgcccg ccctgctgcc gaccgaggac
     3661 tgaccactcg accaggttct gggttgacaa atatcaagac ggaggagatc tctgaagtga
     3721 agatggatgc agaattccga catgactcag gatatgaagt tcatcatcaa aaattggtgt
     3781 tctttgcaga agatgtgggt tcaaacaaag gtgcaatcat tggactcatg gtgggcggtg
     3841 ttgtcatagc gacagtgatc gtcatcacct tggtgatgct gaagaagaaa cagtacacat
     3901 ccattcatca tggtgtggtg gaggttgacg ccgctgtcac cccagaggag cgccacctgt
     3961 ccaagatgca gcagaacggc tacgaaaatc caacctacaa gttctttgag cagatgcaga
     4021 actaggcggc cgcctcgaga gatctttttc cctctgccaa aaattatggg gacatcatga
     4081 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
     4141 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
     4201 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga
     4261 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
     4321 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
     4381 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
     4441 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc
     4501 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     4561 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     4621 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     4681 tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     4741 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     4801 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     4861 gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa
     4921 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     4981 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat
     5041 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     5101 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     5161 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     5221 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5281 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5341 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5401 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5461 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5521 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5581 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5641 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5701 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     5761 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     5821 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     5881 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     5941 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     6001 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6061 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6121 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     6181 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     6241 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     6301 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     6361 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     6421 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6481 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     6541 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6601 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     6661 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     6721 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     6781 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     6841 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     6901 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     6961 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7021 gggtc
//
