LOCUS       sequence-366958-       10138 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible overexpression of SOX9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10138)
  AUTHORS   Romero-Tejeda M, Fonoudi H, Weddle CJ, DeKeyser JM, Lenny B, 
            Fetterman KA, Magdy T, Sapkota Y, Epting C, Burridge PW
  TITLE     A novel transcription factor combination for direct reprogramming to
            a spontaneously contracting human cardiomyocyte-like state.
  JOURNAL   J Mol Cell Cardiol. 2023 Jul 6:S0022-2828(23)00106-2. doi: 
            10.1016/j.yjmcc.2023.06.005.
  PUBMED    37421991
REFERENCE   2  (bases 1 to 10138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Cell 
            Cardiol. 2023 Jul 6:S0022-2828(23)00106-2. doi: 
            10.1016/j.yjmcc.2023.06.005."
COMMENT     Sequence Label: tetO-SOX9
FEATURES             Location/Qualifiers
     source          1..10138
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(49..68)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        240..619
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        621..819
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             837..1017
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1064..1189
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1682..1915
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2100..2144
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2442..2559
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2612..2630,2631..2653,2654..2672,2673..2695,2696..2714,
                     2715..2737,2738..2756,2757..2779,2780..2798,2799..2821,
                     2822..2840,2841..2863,2864..2882)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2915..2953
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2950..2974
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     3053..3072
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3053..3071
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3103..3127
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4664..4688)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4714..4755
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4765..4782
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    4832..5420
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4885..4905)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5303..5314)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5423..5439)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5424..5440)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5945..6125
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6151..6168)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6157..6381
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6427..6855
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6514..6533)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6724..6745
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6864..6884)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6869..7198
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7049..7184
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7111..7130
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7246..7293
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7312..7686
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7816..7937
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7853..7872)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7907..7926
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7986..8002)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7986..8002)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7999..8021)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8010..8026
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8034..8040,8041..8058,8059..8064))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8079..8100
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8217..8234)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8388..8976)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8468..8487)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9147..9938,9939..10007))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9770..9789
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10008..10112)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
       61 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
      121 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
      181 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
      241 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      301 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      361 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      421 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      481 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      541 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      601 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      661 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      721 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      781 gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt
      841 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
      901 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
      961 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     1021 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     1081 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     1141 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     1201 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     1261 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     1321 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     1381 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     1441 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     1501 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
     1561 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     1621 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     1681 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     1741 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     1801 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     1861 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     1921 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     1981 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     2041 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     2101 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     2161 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     2221 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     2281 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     2341 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc
     2401 gtgcgccaat tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
     2461 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     2521 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     2581 agagatccag tttggttatc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     2641 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     2701 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     2761 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     2821 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     2881 gaaaagtgaa agtcgagctc ggtacccggg tcgaggtagg cgtgtacggt gggaggccta
     2941 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
     3001 tgacctccat agaagacacc gggaccgatc cagcctccgc ggccccgaat tgtaatacga
     3061 ctcactatag ggagacccaa gctggctagg taagcttgat caacaagttt gtacaaaaaa
     3121 gcaggcttca ccatgaatct cctggacccc ttcatgaaga tgaccgacga gcaggagaag
     3181 ggcctgtccg gcgcccccag ccccaccatg tccgaggact ccgcgggctc gccctgcccg
     3241 tcgggctccg gctcggacac cgagaacacg cggccccagg agaacacgtt ccccaagggc
     3301 gagcccgatc tgaagaagga gagcgaggag gacaagttcc ccgtgtgcat ccgcgaggcg
     3361 gtcagccagg tgctcaaagg ctacgactgg acgctggtgc ccatgccggt gcgcgtcaac
     3421 ggctccagca agaacaagcc gcacgtcaag cggcccatga acgccttcat ggtgtgggcg
     3481 caggcggcgc gcaggaagct cgcggaccag tacccgcact tgcacaacgc cgagctcagc
     3541 aagacgctgg gcaagctctg gagacttctg aacgagagcg agaagcggcc cttcgtggag
     3601 gaggcggagc ggctgcgcgt gcagcacaag aaggaccacc cggattacaa gtaccagccg
     3661 cggcggagga agtcggtgaa gaacgggcag gcggaggcag aggaggccac ggagcagacg
     3721 cacatctccc ccaacgccat cttcaaggcg ctgcaggccg actcgccaca ctcctcctcc
     3781 ggcatgagcg aggtgcactc ccccggcgag cactcggggc aatcccaggg cccaccgacc
     3841 ccacccacca cccccaaaac cgacgtgcag ccgggcaagg ctgacctgaa gcgagagggg
     3901 cgccccttgc cagagggggg cagacagccc cctatcgact tccgcgacgt ggacatcggc
     3961 gagctgagca gcgacgtcat ctccaacatc gagaccttcg atgtcaacga gtttgaccag
     4021 tacctgccgc ccaacggcca cccgggggtg ccggccacgc acggccaggt cacctacacg
     4081 ggcagctacg gcatcagcag caccgcggcc accccggcga gcgcgggcca cgtgtggatg
     4141 tccaagcagc aggcgccgcc gccacccccg cagcagcccc cacaggcccc gccggccccg
     4201 caggcgcccc cgcagccgca ggcggcgccc ccacagcagc cggcggcacc cccgcagcag
     4261 ccacaggcgc acacgctgac cacgctgagc agcgagccgg gccagtccca gcgaacgcac
     4321 atcaagacgg agcagctgag ccccagccac tacagcgagc agcagcagca ctcgccccaa
     4381 cagatcgcct acagcccctt caacctccca cactacagcc cctcctaccc gcccatcacc
     4441 cgctcacagt acgactacac cgaccaccag aactccagct cctactacag ccacgcggca
     4501 ggccagggca ccggcctcta ctccaccttc acctacatga accccgctca gcgccccatg
     4561 tacaccccca tcgccgacac ctctggggtc ccttccatcc cgcagaccca cagcccccag
     4621 cactgggaac aacccgtcta cacacagctc actcgacctt gaaacccagc tttcttgtac
     4681 aaagtggttg atctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc
     4741 ggtctcgatt ctacgcgtac cggtcatcat caccatcacc attgagttta aacccgctga
     4801 tcagcctcaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     4861 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4921 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4981 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     5041 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     5101 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     5161 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     5221 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     5281 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     5341 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     5401 cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc
     5461 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     5521 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     5581 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     5641 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     5701 tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg
     5761 ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac
     5821 ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga
     5881 gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg
     5941 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     6001 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     6061 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     6121 tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     6181 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     6241 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     6301 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     6361 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     6421 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     6481 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     6541 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     6601 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     6661 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     6721 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     6781 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     6841 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     6901 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     6961 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     7021 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     7081 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     7141 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     7201 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
     7261 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
     7321 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
     7381 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
     7441 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
     7501 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
     7561 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
     7621 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
     7681 gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     7741 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     7801 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7861 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7921 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     7981 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     8041 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     8101 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     8161 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     8221 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     8281 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     8341 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     8401 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     8461 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     8521 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     8581 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     8641 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8701 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8761 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8821 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8881 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8941 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     9001 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     9061 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     9121 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     9181 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     9241 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     9301 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     9361 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     9421 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     9481 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     9541 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9601 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     9661 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9721 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     9781 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9841 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9901 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9961 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    10021 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    10081 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctg
//
