LOCUS       sequence-36698-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Sato S, Tomomori-Sato C, Banks CA, Parmely TJ, Sorokina I, Brower 
            CS, Conaway RC, Conaway JW
  TITLE     A mammalian homolog of Drosophila melanogaster transcriptional 
            coactivator intersex is a subunit of the mammalian Mediator complex.
  JOURNAL   J Biol Chem. 2003 Dec 12. 278(50):49671-4.
  PUBMED    14576168
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Dec 12. 278(50):49671-4."
COMMENT     Sequence Label: pET19b-MED17
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..85
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             101..115
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 gagnaattcc ctctanaata ttttgtttac tttaagaagg agatatacca tgggccatca
       61 tcatcatcat catcatcatc atcacagcag cggccatatc gacgacgacg acaagcatat
      121 gctcgagatg tccggggtgc gcgcagtgcg gatcagcatc gaatcggcct gcgagaagca
      181 ggtccatgag gtgggcctgg atggcaccga gacgtacctg cccccgctgt ccatgtcgca
      241 gaatctggcg cgtctggccc agcggataga cttcagccag ggttcgggct ccgaggagga
      301 ggaggcggcg gggaccgagg gcgacgcgca ggagtggccg ggcgccgggt ccagcgcaga
      361 ccaggacgac gaggaaggag tggtaaaatt tcagccttcc ctttggcctt gggactcagt
      421 gaggaacaat ttgagaagtg ccctgacaga gatgtgtgtt ctctatgatg ttctcagtat
      481 tgttagggat aaaaaattta tgactcttga tcctgtctct caggatgcac ttcctccaaa
      541 acagaatcct cagacgttgc aattgatatc taaaaagaag tcacttgctg gagcagcaca
      601 aatcttattg aagggggcag aaagactgac taaatcagtt accgaaaacc aagaaaacaa
      661 gctacaaaga gacttcaatt ctgagctttt gcgattacgg caacactgga aacttcgaaa
      721 agttggagat aaaattctcg gagatctgag ctacagaagt gcaggatctc tctttcctca
      781 tcatggtaca tttgaagtaa taaagaatac agatctcgat ctggataaaa agatacctga
      841 agattactgt cctcttgatg tccaaattcc tagtgattta gaggggtctg catatatcaa
      901 ggtttcaata caaaaacagg ctccagat
//
