LOCUS       sequence-366993-        9740 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin 
            inducible degron) and 3HA. ARF16(PB1)-P2A-osTIR1 with SIR4 homology 
            arms for genome integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9740)
  AUTHORS   Shively CA, Dong F, Mitra RD
  TITLE     A Suite of New Strain Construction Vectors for Gene Expression 
            Knockdown in Budding Yeast.
  JOURNAL   ACS Synth Biol. 2023 Jan 17. doi: 10.1021/acssynbio.2c00547.
  PUBMED    36650116
REFERENCE   2  (bases 1 to 9740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Jan 17. doi: 10.1021/acssynbio.2c00547."
COMMENT     Sequence Label: C-SWAT IAA17-3HA
FEATURES             Location/Qualifiers
     source          1..9740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             440..1774
                     /codon_start=1
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /product="O-acetylserine and O-acetylhomoserine 
                     sulfhydrylase, required for sulfur amino acid synthesis"
                     /label=MET17
                     /translation="MPSHFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGSQ
                     LFGLEVPGYVYSRFQNPTSNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGDNI
                     VSTSYLYGGTYNQFKISFKRFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPKYNV
                     PDFEKIVAIAHKHGIPVVVDNTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTIGGII
                     VDSGKFPWKDYPEKFPQFSQPAEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPLMNPFA
                     SFLLLQGVETLSLRAERHGENALKLAKWLEQSPYVSWVSYPGLASHSHHENAKKYLSNG
                     FGGVLSFGVKDLPNADKETDPFELSGAQVVDNLKLASNLANVGDAKTLVIAPYFTTHKQ
                     LNDKEKLASGVTKDLIRVSVGIEFIDDIIADFQQSFETVFAGQKP"
     rep_origin      complement(1879..2334)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2016..2037)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2228..2247
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2460..2482
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2474..2491
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2475..2491
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2501..2520
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2501..2519
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2544..2560
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2545..2561
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2655..3335
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="GSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLNNE
                     PANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGG
                     PEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFMNE
                     RKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAMEK
                     CKSRA"
     CDS             3351..3440
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     promoter        3905..4306
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             4328..4348
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4886..4912
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4922..4978
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5015..5035
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5051..6772
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="TYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVG
                     NCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEEL
                     RMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEVE
                     DRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTLA
                     KILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYPL
                     CAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELRV
                     FPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLC
                     ILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIA
                     FAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKGC
                     QVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     primer_bind     complement(6967..6987)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6967..6985)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7006..7022)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7006..7022)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7019..7041)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7030..7046
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7054..7060,7061..7078,7079..7084))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7099..7120
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7237..7254)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7408..7996)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7488..7507)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8167..8958,8959..9027))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8790..8809
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9028..9132)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    9169..9672
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     9713..9731
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accacgcttt tcaattcaat tcatcaccat cctcatgaaa actgtgtaac ataataaccg
      241 aagtgtcgaa aaggtggcac cttgtccaat tgaacacgct cgatgaaaaa aataagatat
      301 atataaggtt aagtaaagcg tctgttagaa aggaagtttt tcctttttct tgctctcttg
      361 tcttttcatc tactatttcc ttcgtgtaat acagggtcgt cagatacata gatacaattc
      421 tattaccccc atccatacaa tgccatctca tttcgatact gttcaactac acgccggcca
      481 agagaaccct ggtgacaatg ctcacagatc cagagctgta ccaatttacg ccaccacttc
      541 ttatgttttc gaaaactcta agcatggttc gcaattgttt ggtctagaag ttccaggtta
      601 cgtctattcc cgtttccaaa acccaaccag taatgttttg gaagaaagaa ttgctgcctt
      661 agaaggtggt gctgctgctt tggctgtttc ctccggtcaa gccgctcaaa cccttgccat
      721 ccaaggtttg gcacacactg gtgacaacat cgtttccact tcttacttat acggtggtac
      781 ttataaccag ttcaaaatct cgttcaaaag atttggtatc gaggctagat ttgttgaagg
      841 tgacaatcca gaagaattcg aaaaggtctt tgatgaaaga accaaggctg tttatttgga
      901 aaccattggt aatccaaagt acaatgttcc ggattttgaa aaaattgttg caattgctca
      961 caaacacggt attccagttg tcgttgacaa cacatttggt gccggtggtt acttctgtca
     1021 gccaattaaa tacggtgctg atattgtaac acattctgct accaaatgga ttggtggtca
     1081 tggtactact atcggtggta ttattgttga ctctggtaag ttcccatgga aggactaccc
     1141 agaaaagttc cctcaattct ctcaacctgc cgaaggatat cacggtacta tctacaatga
     1201 agcctacggt aacttggcat acatcgttca tgttagaact gaactattaa gagatttggg
     1261 tccattgatg aacccatttg cctctttctt gctactacaa ggtgttgaaa cattatcttt
     1321 gagagctgaa agacacggtg aaaatgcatt gaagttagcc aaatggttag aacaatcccc
     1381 atacgtatct tgggtttcat accctggttt agcatctcat tctcatcatg aaaatgctaa
     1441 gaagtatcta tctaacggtt tcggtggtgt cttatctttc ggtgtaaaag acttaccaaa
     1501 tgccgacaag gaaactgacc cattcgaact ttctggtgct caagttgttg acaatttaaa
     1561 gcttgcctct aacttggcca atgttggtga tgccaagacc ttagtcattg ctccatactt
     1621 cactacccac aaacaattaa atgacaaaga aaagttggca tctggtgtta ccaaggactt
     1681 aattcgtgtc tctgttggta tcgaatttat tgatgacatt attgcagact tccagcaatc
     1741 ttttgaaact gttttcgctg gccaaaaacc atgagtgtgc gtgatgagtt gtaaaattat
     1801 gtataaacct actttctctc acaagtactg cggtgtgaaa taccgcacag atgcgtaagg
     1861 agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     1921 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     1981 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     2041 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     2101 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     2161 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     2221 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     2281 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc
     2341 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     2401 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     2461 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
     2521 cgaattgggt accgggcccc ccctcgaggt cgacggtatc gataagcttg atatcgaatt
     2581 cctgcagcta tattaccctg ttatccctac gtacgctgca ggtcgacggt ggcggttctg
     2641 gcggtggcgg atccggctct gttgagttaa acctgcgtga gaccgagcta tgtcttggtc
     2701 tacccggtgg tgacactgta gcaccagtga caggcaacaa aaggggcttc agcgagactg
     2761 tagatctgaa gctaaactta aacaacgaac cagctaataa ggaaggtagt acaacacatg
     2821 atgtggttac gtttgacagc aaggagaaga gtgcctgtcc caaagatccg gcaaagcccc
     2881 cggcgaaggc ccaggttgta ggttggcctc cggtaaggtc ctatcgtaaa aacgtaatgg
     2941 ttagttgtca gaagtccagc ggaggacctg aagcagcggc gttcgtcaaa gtatctatgg
     3001 atggcgcccc atacttgaga aagatagacc tgcgtatgta taagtcttac gacgagttat
     3061 caaatgcgct atctaacatg ttcagcagtt tcaccatggg caaacatggt ggtgaggagg
     3121 gaatgatcga cttcatgaac gagagaaaac ttatggacct ggtgaatagc tgggattatg
     3181 tccctagcta cgaggataaa gacggggatt ggatgcttgt gggggatgtt ccgtggccga
     3241 tgttcgtcga tacgtgtaag cgtctgcgtc taatgaaggg ctccgatgct attggtttag
     3301 ctccgcgtgc tatggaaaag tgtaagtcca gagctgtatc acccggggca tacccatacg
     3361 atgttcctga ctatgcgggc tatccctatg acgtcccgga ctatgcagga tcctatccat
     3421 atgacgttcc agattacgct gcttgactta agagttcttc tttgagatat cgattgaacg
     3481 agctcgaatt catcgatcta tattaccctg ttatccctag cgtaactatg ccaaatgaca
     3541 ataagacacc caataggtcc agcactccca agtttactaa aaaaccagta accccgaatg
     3601 ataaaattcc tgaaagacgg gtgtacaata tggacttcct cttttctggc aaccaaaccc
     3661 atacatcggg attcctataa taccttcgtt ggtctcccta acatgtaggt ggcggagggg
     3721 agatatacaa tagaacagat accagacaag acataatggg ctaaacaaga ctacaccaat
     3781 tacactgcct cattgatggt ggtacataac gaactaatac tgtagcccta gacttgatag
     3841 ccatcatcat atcgaagttt cactaccctt tttccatttg ccatctattg aagtaataat
     3901 aggcgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
     3961 atatccgcaa tgacaaaaaa atgatggaag acactaaagg aaaaaattaa cgacaaagac
     4021 agcaccaaca gatgtcgttg ttccagagct gatgaggggt atctcgaagc acacgaaact
     4081 ttttccttcc ttcattcacg cacactactc tctaatgagc aacggtatac ggccttcctt
     4141 ccagttactt gaatttgaaa taaaaaaaag tttgctgtct tgctatcaag tataaataga
     4201 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
     4261 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactatct catatacaac
     4321 tagaatgccc aagaagaaac gtaaggtggg ttccaccctt acgagcggtc aacgtgacca
     4381 cagagacgtt gaaaatgagt tatccagtgc agcttttagc agtcaaagtt tcggggttcc
     4441 agatatgtca ttcaaaccgg ggtgctccag tgacgtcgca gtcactgatg cgggtatgcc
     4501 tagccaaggg ttatggaaca accagacgca aaggatgcgt accttcacta aggtccagaa
     4561 aaggggctct gttgggagga gtatcgacat caccaggtat cgtgattacg atgagctacg
     4621 tcatgatctg gcatgtatgt tcggcataca ggggcagtta gaagaccctt atcgtatgga
     4681 ttggaagtta gtttatgtgg atcacgagaa cgatatactg ttggtgggcg acgacccgtg
     4741 ggaggaattt gtaggttgtg ttaagtctat caagattcta tctgctgcag aggtgcagca
     4801 aatgagcttg gacggggatc ttggtggagt tccgccccaa acccaggcgt gcagtgcttc
     4861 agacgatgcg aacgcttgga gaggctatcc ctacgacgtg cctgattatg cgggtagcgg
     4921 ggccactaac ttcagcttgt tgaagcaggc tggcgatgtg gaggagaatc caggtccggc
     4981 ggctaccgat aaagcggaat taattcccga gcctccaaaa aagaagagaa aggtcgaatt
     5041 gggtaccgcc acgtacttcc cggaggaggt ggtggagcac atcttcagct tcctgccggc
     5101 gcagcgcgac cgcaacacgg tctcgctcgt ctgcaaggtg tggtacgaga tcgagaggct
     5161 gagccgccgc ggcgtcttcg tgggcaactg ctacgccgtg cgcgccggcc gcgtcgccgc
     5221 gcggttcccc aacgtgcggg cgctcacggt gaaggggaag ccccacttcg ccgacttcaa
     5281 cctcgtgccc cccgactggg gcggctacgc ggggccgtgg atcgaggcgg ccgcgagggg
     5341 atgccacggc ctggaggagc tcaggatgaa gcggatggtg gtgtccgacg agagcctcga
     5401 gctgctggct cgctcgttcc cgcggttcag ggctcttgtt cttatcagct gcgaggggtt
     5461 cagcactgac gggctagccg ccgtcgcgag ccattgcaag cttctgaggg agttggattt
     5521 gcaggaaaat gaagtggagg atcgagggcc taggtggctt tcctgcttcc ctgattcctg
     5581 cacatcactt gtctcattga attttgcctg catcaaaggg gaggttaatg ctggttcact
     5641 ggagagactt gttagcaggt ccccaaacct gcggagtttg aggctgaatc gatctgtatc
     5701 ggtagataca cttgcaaaga tactactgcg tacccctaac ttggaggatt tggggacagg
     5761 gaatttgaca gatgacttcc aaactgagtc ctactttaag cttaccagtg ctctggagaa
     5821 atgcaagatg ttgaggagtt tgtctggatt ctgggatgct tctcctgttt gcctgtcatt
     5881 tatctacccc ctgtgtgctc aactgacagg attgaacttg agctatgcac ccacacttga
     5941 tgcttctgac cttacaaaaa tgattagccg ctgtgtgaag ctccaacgcc tttgggtact
     6001 ggattgtatc tcggacaaag gcttgcaagt ggtggcctcc agttgcaaag acttgcaaga
     6061 actcagggta tttccatcag atttctacgt agctggttat tctgcagtga cagaggaggg
     6121 acttgttgca gtatccttgg gctgtccaaa actgaactca ctactgtact tctgtcacca
     6181 aatgactaat gctgcactag ttactgtcgc caagaactgt ccaaatttca cacgattcag
     6241 actttgtatt cttgagccag ggaagcctga tgttgtgaca agccaaccat tagatgaagg
     6301 ctttggagct attgttcgtg agtgcaaggg attacaacgt ttgtcaatat ctggtcttct
     6361 cacagacaaa gttttcatgt atattgggaa atatgcaaaa caacttgaga tgctttctat
     6421 agcatttgct ggtgacagtg ataagggtat gatgcatgtt atgaatggat gcaagaattt
     6481 aaggaaactg gagataagag atagcccgtt tggtgatgct gcactcttgg ggaattttgc
     6541 taggtacgag acaatgcgat ccctttggat gtcatcttgc aatgtcacgt taaaggggtg
     6601 ccaagtcctt gcgtcaaaga tgccgatgct caatgttgag gtcataaatg agcgggatgg
     6661 tagcaatgaa atggaggaaa accatggaga tctgcctaaa gtggagaaat tatatgtgta
     6721 ccgcacaact gctggggcga gggatgatgc accaaatttt gttaaaatcc tagtcgactc
     6781 cggttctgct gctagtggtt gaacgcgtgg cgcctggaaa aagattttca agtgaataag
     6841 gagataaaac cgtattgact ttttatcatt ctacaaaaga ccagtaacga agtgtaccct
     6901 gctatattac cctgttatcc ctagcgtaac tcggccgcca ccgcggtgga gctccagctt
     6961 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     7021 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7081 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7141 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7201 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7261 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7321 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7381 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7441 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7501 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7561 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7621 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7681 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7741 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7801 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7861 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7921 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7981 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8041 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8101 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8161 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8221 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8281 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8341 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8401 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8461 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8521 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8581 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8641 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8701 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8761 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8821 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8881 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8941 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9001 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9061 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9121 aggggttccg cgcacatttc cccgaaaagt gccacctggg tccttttcat cacgtgctat
     9181 aaaaataatt ataatttaaa ttttttaata taaatatata aattaaaaat agaaagtaaa
     9241 aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga tgtaaaagac tctaggggga
     9301 tcgccaacaa atactacctt ttatcttgct cttcctgctc tcaggtatta atgccgaatt
     9361 gtttcatctt gtctgtgtag aagaccacac acgaaaatcc tgtgatttta cattttactt
     9421 atcgttaatc gaatgtatat ctatttaatc tgcttttctt gtctaataaa tatatatgta
     9481 aagtacgctt tttgttgaaa ttttttaaac ctttgtttat ttttttttct tcattccgta
     9541 actcttctac cttctttatt tactttctaa aatccaaata caaaacataa aaataaataa
     9601 acacagagta aattcccaaa ttattccatc attaaaagat acgaggcgcg tgtaagttac
     9661 aggcaagcga tccgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     9721 tatcacgagg ccctttcgtc
//
