LOCUS       sequence-36701-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Whitmarsh AJ, Shore P, Sharrocks AD, Davis RJ
  TITLE     Integration of MAP kinase signal transduction pathways at the serum 
            response element.
  JOURNAL   Science. 1995 Jul 21. 269(5222):403-7.
  PUBMED    7618106
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1995 Jul 21. 269(5222):403-7."
COMMENT     Sequence Label: pCDNA3 Flag MKK4(glu)
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     59..78
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        59..77
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             122..145
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgngggcnnt gcgactctct ggctactaga gacccactgc ttactggctt atcgaaatta
       61 atacgactca ctatagggag acccaagctt ggtaccgagc tcggatccgg cagccaacat
      121 ggactataag gacgatgacg acaaacaggg taaacgcaaa gcactgaagt tgaattttgc
      181 aaatccacct ttcaaatcta cagcaaggtt tactctgaat cccaatccta caggagttca
      241 aaacccacac atagagagac tgagaacaca cagcattgag tcatcaggaa aactgaagat
      301 ctcccctgaa caacactggg atttcactgc agaggacttg aaagaccttg gagaaattgg
      361 acgaggagct tatggttctg tcaacaaaat ggtccacaaa ccaagtgggc aaataatggc
      421 agttaaaaga attcggtcaa cagtggatga aaaagaacaa aaacaacttc ttatggattt
      481 ggatgtagta atgcggagta gtgattgccc atacattgtt cagttttatg gtgcactctt
      541 cagagagggt gactgttgga tctgtatgga actcatgtct acctcgtttg ataagtttta
      601 caaatatgta tatagtgtat tagatgatgt tattccagaa gaaattttag gcaaaatcac
      661 tttagcaact gtgaaagcac taaaccactt aaaagaaaac ttgaaaatta ttcacagaga
      721 tatcaaacct tccaatattc ttctggacag aagtggaaat atcaagctct gtgacttcgg
      781 catcagtgga cagcttgtag acgagattgc caaggaaaga gatgctggct gtaggccata
      841 catggcacct gaaagaatag acccaagcgc atcacgacaa ggatatgatg tccgctctga
      901 tgtctggagt tgggacnntt gtatgantgc ccaggcgatt cttatcnagt ggatatgatt
      961 gacactac
//
