LOCUS       sequence-367017-        8668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-SWAT compatible vector for C-terminal tagging with AID*-6FLAG for 
            auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology 
            arms for integration into the genome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8668)
  AUTHORS   Shively CA, Dong F, Mitra RD
  TITLE     A Suite of New Strain Construction Vectors for Gene Expression 
            Knockdown in Budding Yeast.
  JOURNAL   ACS Synth Biol. 2023 Jan 17. doi: 10.1021/acssynbio.2c00547.
  PUBMED    36650116
REFERENCE   2  (bases 1 to 8668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Jan 17. doi: 10.1021/acssynbio.2c00547."
COMMENT     Sequence Label: C-SWAT AID*-6FLAG osTIR1
FEATURES             Location/Qualifiers
     source          1..8668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             440..1774
                     /codon_start=1
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /product="O-acetylserine and O-acetylhomoserine 
                     sulfhydrylase, required for sulfur amino acid synthesis"
                     /label=MET17
                     /translation="MPSHFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGSQ
                     LFGLEVPGYVYSRFQNPTSNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGDNI
                     VSTSYLYGGTYNQFKISFKRFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPKYNV
                     PDFEKIVAIAHKHGIPVVVDNTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTIGGII
                     VDSGKFPWKDYPEKFPQFSQPAEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPLMNPFA
                     SFLLLQGVETLSLRAERHGENALKLAKWLEQSPYVSWVSYPGLASHSHHENAKKYLSNG
                     FGGVLSFGVKDLPNADKETDPFELSGAQVVDNLKLASNLANVGDAKTLVIAPYFTTHKQ
                     LNDKEKLASGVTKDLIRVSVGIEFIDDIIADFQQSFETVFAGQKP"
     rep_origin      complement(1879..2334)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2016..2037)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2228..2247
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2460..2482
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2474..2491
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2475..2491
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2501..2520
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2501..2519
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2544..2560
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2545..2561
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2655..2786
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             2832..2855
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2862..2885
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2892..2915
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2922..2945
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2952..2975
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2982..3005
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        3493..3894
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             3943..3963
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3979..5700
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="TYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVG
                     NCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEEL
                     RMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEVE
                     DRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTLA
                     KILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYPL
                     CAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELRV
                     FPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLC
                     ILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIA
                     FAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKGC
                     QVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     primer_bind     complement(5895..5915)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5895..5913)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5934..5950)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5934..5950)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5947..5969)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5958..5974
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5982..5988,5989..6006,6007..6012))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6027..6048
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6165..6182)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6336..6924)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6416..6435)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7095..7886,7887..7955))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7718..7737
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7956..8060)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    8097..8600
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     8641..8659
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accacgcttt tcaattcaat tcatcaccat cctcatgaaa actgtgtaac ataataaccg
      241 aagtgtcgaa aaggtggcac cttgtccaat tgaacacgct cgatgaaaaa aataagatat
      301 atataaggtt aagtaaagcg tctgttagaa aggaagtttt tcctttttct tgctctcttg
      361 tcttttcatc tactatttcc ttcgtgtaat acagggtcgt cagatacata gatacaattc
      421 tattaccccc atccatacaa tgccatctca tttcgatact gttcaactac acgccggcca
      481 agagaaccct ggtgacaatg ctcacagatc cagagctgta ccaatttacg ccaccacttc
      541 ttatgttttc gaaaactcta agcatggttc gcaattgttt ggtctagaag ttccaggtta
      601 cgtctattcc cgtttccaaa acccaaccag taatgttttg gaagaaagaa ttgctgcctt
      661 agaaggtggt gctgctgctt tggctgtttc ctccggtcaa gccgctcaaa cccttgccat
      721 ccaaggtttg gcacacactg gtgacaacat cgtttccact tcttacttat acggtggtac
      781 ttataaccag ttcaaaatct cgttcaaaag atttggtatc gaggctagat ttgttgaagg
      841 tgacaatcca gaagaattcg aaaaggtctt tgatgaaaga accaaggctg tttatttgga
      901 aaccattggt aatccaaagt acaatgttcc ggattttgaa aaaattgttg caattgctca
      961 caaacacggt attccagttg tcgttgacaa cacatttggt gccggtggtt acttctgtca
     1021 gccaattaaa tacggtgctg atattgtaac acattctgct accaaatgga ttggtggtca
     1081 tggtactact atcggtggta ttattgttga ctctggtaag ttcccatgga aggactaccc
     1141 agaaaagttc cctcaattct ctcaacctgc cgaaggatat cacggtacta tctacaatga
     1201 agcctacggt aacttggcat acatcgttca tgttagaact gaactattaa gagatttggg
     1261 tccattgatg aacccatttg cctctttctt gctactacaa ggtgttgaaa cattatcttt
     1321 gagagctgaa agacacggtg aaaatgcatt gaagttagcc aaatggttag aacaatcccc
     1381 atacgtatct tgggtttcat accctggttt agcatctcat tctcatcatg aaaatgctaa
     1441 gaagtatcta tctaacggtt tcggtggtgt cttatctttc ggtgtaaaag acttaccaaa
     1501 tgccgacaag gaaactgacc cattcgaact ttctggtgct caagttgttg acaatttaaa
     1561 gcttgcctct aacttggcca atgttggtga tgccaagacc ttagtcattg ctccatactt
     1621 cactacccac aaacaattaa atgacaaaga aaagttggca tctggtgtta ccaaggactt
     1681 aattcgtgtc tctgttggta tcgaatttat tgatgacatt attgcagact tccagcaatc
     1741 ttttgaaact gttttcgctg gccaaaaacc atgagtgtgc gtgatgagtt gtaaaattat
     1801 gtataaacct actttctctc acaagtactg cggtgtgaaa taccgcacag atgcgtaagg
     1861 agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     1921 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     1981 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     2041 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     2101 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     2161 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     2221 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     2281 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc
     2341 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     2401 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     2461 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
     2521 cgaattgggt accgggcccc ccctcgaggt cgacggtatc gataagcttg atatcgaatt
     2581 cctgcagcta tattaccctg ttatccctac gtacgctgca ggtcgacggt ggcggttctg
     2641 gcggtggcgg atcccctaaa gatccagcca aacctccggc caaggcacaa gttgtgggat
     2701 ggccaccggt gagatcatac cggaagaacg tgatggtttc ctgccaaaaa tcaagcggtg
     2761 gcccggaggc ggcggcgttc gtgaaggtat cacccgggat acattatacg aagttatgca
     2821 tgctcactag tgactacaag gacgacgatg acaagtctag tgactacaag gacgacgatg
     2881 acaagtctag tgactacaag gacgacgatg acaagtctag tgactacaag gacgacgatg
     2941 acaagtctag tgactacaag gacgacgatg acaagtctag tgactacaag gacgacgatg
     3001 acaagtctag atgaggcgcg ctagatctgt ttagcttaag agttcttctt tgagatatcg
     3061 attgaacgag ctcgaattca tcgatctata ttaccctgtt atccctagcg taactatgcc
     3121 aaatgacaat aagacaccca ataggtccag cactcccaag tttactaaaa aaccagtaac
     3181 cccgaatgat aaaattcctg aaagacgggt gtacaatatg gacttcctct tttctggcaa
     3241 ccaaacccat acatcgggat tcctataata ccttcgttgg tctccctaac atgtaggtgg
     3301 cggaggggag atatacaata gaacagatac cagacaagac ataatgggct aaacaagact
     3361 acaccaatta cactgcctca ttgatggtgg tacataacga actaatactg tagccctaga
     3421 cttgatagcc atcatcatat cgaagtttca ctaccctttt tccatttgcc atctattgaa
     3481 gtaataatag gcgcatgcaa cttcttttct ttttttttct tttctctctc ccccgttgtt
     3541 gtctcaccat atccgcaatg acaaaaaaat gatggaagac actaaaggaa aaaattaacg
     3601 acaaagacag caccaacaga tgtcgttgtt ccagagctga tgaggggtat ctcgaagcac
     3661 acgaaacttt ttccttcctt cattcacgca cactactctc taatgagcaa cggtatacgg
     3721 ccttccttcc agttacttga atttgaaata aaaaaaagtt tgctgtcttg ctatcaagta
     3781 taaatagacc tgcaattatt aatcttttgt ttcctcgtca ttgttctcgt tccctttctt
     3841 ccttgtttct ttttctgcac aatatttcaa gctataccaa gcatacaatc aactatctca
     3901 tatacaacta gaatggataa agcggaatta attcccgagc ctccaaaaaa gaagagaaag
     3961 gtcgaattgg gtaccgccac gtacttcccg gaggaggtgg tggagcacat cttcagcttc
     4021 ctgccggcgc agcgcgaccg caacacggtc tcgctcgtct gcaaggtgtg gtacgagatc
     4081 gagaggctga gccgccgcgg cgtcttcgtg ggcaactgct acgccgtgcg cgccggccgc
     4141 gtcgccgcgc ggttccccaa cgtgcgggcg ctcacggtga aggggaagcc ccacttcgcc
     4201 gacttcaacc tcgtgccccc cgactggggc ggctacgcgg ggccgtggat cgaggcggcc
     4261 gcgaggggat gccacggcct ggaggagctc aggatgaagc ggatggtggt gtccgacgag
     4321 agcctcgagc tgctggctcg ctcgttcccg cggttcaggg ctcttgttct tatcagctgc
     4381 gaggggttca gcactgacgg gctagccgcc gtcgcgagcc attgcaagct tctgagggag
     4441 ttggatttgc aggaaaatga agtggaggat cgagggccta ggtggctttc ctgcttccct
     4501 gattcctgca catcacttgt ctcattgaat tttgcctgca tcaaagggga ggttaatgct
     4561 ggttcactgg agagacttgt tagcaggtcc ccaaacctgc ggagtttgag gctgaatcga
     4621 tctgtatcgg tagatacact tgcaaagata ctactgcgta cccctaactt ggaggatttg
     4681 gggacaggga atttgacaga tgacttccaa actgagtcct actttaagct taccagtgct
     4741 ctggagaaat gcaagatgtt gaggagtttg tctggattct gggatgcttc tcctgtttgc
     4801 ctgtcattta tctaccccct gtgtgctcaa ctgacaggat tgaacttgag ctatgcaccc
     4861 acacttgatg cttctgacct tacaaaaatg attagccgct gtgtgaagct ccaacgcctt
     4921 tgggtactgg attgtatctc ggacaaaggc ttgcaagtgg tggcctccag ttgcaaagac
     4981 ttgcaagaac tcagggtatt tccatcagat ttctacgtag ctggttattc tgcagtgaca
     5041 gaggagggac ttgttgcagt atccttgggc tgtccaaaac tgaactcact actgtacttc
     5101 tgtcaccaaa tgactaatgc tgcactagtt actgtcgcca agaactgtcc aaatttcaca
     5161 cgattcagac tttgtattct tgagccaggg aagcctgatg ttgtgacaag ccaaccatta
     5221 gatgaaggct ttggagctat tgttcgtgag tgcaagggat tacaacgttt gtcaatatct
     5281 ggtcttctca cagacaaagt tttcatgtat attgggaaat atgcaaaaca acttgagatg
     5341 ctttctatag catttgctgg tgacagtgat aagggtatga tgcatgttat gaatggatgc
     5401 aagaatttaa ggaaactgga gataagagat agcccgtttg gtgatgctgc actcttgggg
     5461 aattttgcta ggtacgagac aatgcgatcc ctttggatgt catcttgcaa tgtcacgtta
     5521 aaggggtgcc aagtccttgc gtcaaagatg ccgatgctca atgttgaggt cataaatgag
     5581 cgggatggta gcaatgaaat ggaggaaaac catggagatc tgcctaaagt ggagaaatta
     5641 tatgtgtacc gcacaactgc tggggcgagg gatgatgcac caaattttgt taaaatccta
     5701 gtcgactccg gttctgctgc tagtggttga acgcgtggcg cctggaaaaa gattttcaag
     5761 tgaataagga gataaaaccg tattgacttt ttatcattct acaaaagacc agtaacgaag
     5821 tgtaccctgc tatattaccc tgttatccct agcgtaactc ggccgccacc gcggtggagc
     5881 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
     5941 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6001 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6061 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6121 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6181 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6241 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6301 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6361 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6421 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6481 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6541 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6601 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6661 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6721 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     6781 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     6841 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     6901 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     6961 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7021 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7081 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7141 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7201 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7261 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7321 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7381 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     7441 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7501 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7561 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7621 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7681 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     7741 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     7801 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     7861 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     7921 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     7981 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8041 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc cttttcatca
     8101 cgtgctataa aaataattat aatttaaatt ttttaatata aatatataaa ttaaaaatag
     8161 aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt tccgaagatg taaaagactc
     8221 tagggggatc gccaacaaat actacctttt atcttgctct tcctgctctc aggtattaat
     8281 gccgaattgt ttcatcttgt ctgtgtagaa gaccacacac gaaaatcctg tgattttaca
     8341 ttttacttat cgttaatcga atgtatatct atttaatctg cttttcttgt ctaataaata
     8401 tatatgtaaa gtacgctttt tgttgaaatt ttttaaacct ttgtttattt ttttttcttc
     8461 attccgtaac tcttctacct tctttattta ctttctaaaa tccaaataca aaacataaaa
     8521 ataaataaac acagagtaaa ttcccaaatt attccatcat taaaagatac gaggcgcgtg
     8581 taagttacag gcaagcgatc cgtctaagaa accattatta tcatgacatt aacctataaa
     8641 aataggcgta tcacgaggcc ctttcgtc
//
