LOCUS       sequence-367018-        9877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-SWAT compatible vector for C-terminal tagging with IAA17 
            (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 
            homology arms for genome integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9877)
  AUTHORS   Shively CA, Dong F, Mitra RD
  TITLE     A Suite of New Strain Construction Vectors for Gene Expression 
            Knockdown in Budding Yeast.
  JOURNAL   ACS Synth Biol. 2023 Jan 17. doi: 10.1021/acssynbio.2c00547.
  PUBMED    36650116
REFERENCE   2  (bases 1 to 9877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Jan 17. doi: 10.1021/acssynbio.2c00547."
COMMENT     Sequence Label: C-SWAT IAA17-6FLAG
FEATURES             Location/Qualifiers
     source          1..9877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             440..1774
                     /codon_start=1
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /product="O-acetylserine and O-acetylhomoserine 
                     sulfhydrylase, required for sulfur amino acid synthesis"
                     /label=MET17
                     /translation="MPSHFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGSQ
                     LFGLEVPGYVYSRFQNPTSNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGDNI
                     VSTSYLYGGTYNQFKISFKRFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPKYNV
                     PDFEKIVAIAHKHGIPVVVDNTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTIGGII
                     VDSGKFPWKDYPEKFPQFSQPAEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPLMNPFA
                     SFLLLQGVETLSLRAERHGENALKLAKWLEQSPYVSWVSYPGLASHSHHENAKKYLSNG
                     FGGVLSFGVKDLPNADKETDPFELSGAQVVDNLKLASNLANVGDAKTLVIAPYFTTHKQ
                     LNDKEKLASGVTKDLIRVSVGIEFIDDIIADFQQSFETVFAGQKP"
     rep_origin      complement(1879..2334)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2016..2037)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2228..2247
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2460..2482
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2474..2491
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2475..2491
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2501..2520
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2501..2519
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2544..2560
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2545..2561
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2655..3335
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="GSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLNNE
                     PANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGG
                     PEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFMNE
                     RKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAMEK
                     CKSRA"
     CDS             3381..3404
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3411..3434
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3441..3464
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3471..3494
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3501..3524
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3531..3554
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        4042..4443
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             4465..4485
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5023..5049
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5059..5115
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5152..5172
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5188..6909
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="TYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVG
                     NCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEEL
                     RMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEVE
                     DRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTLA
                     KILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYPL
                     CAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELRV
                     FPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLC
                     ILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIA
                     FAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKGC
                     QVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     primer_bind     complement(7104..7124)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7104..7122)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7143..7159)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7143..7159)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7156..7178)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7167..7183
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7191..7197,7198..7215,7216..7221))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7236..7257
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7374..7391)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7545..8133)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7625..7644)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8304..9095,9096..9164))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8927..8946
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9165..9269)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    9306..9809
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     9850..9868
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accacgcttt tcaattcaat tcatcaccat cctcatgaaa actgtgtaac ataataaccg
      241 aagtgtcgaa aaggtggcac cttgtccaat tgaacacgct cgatgaaaaa aataagatat
      301 atataaggtt aagtaaagcg tctgttagaa aggaagtttt tcctttttct tgctctcttg
      361 tcttttcatc tactatttcc ttcgtgtaat acagggtcgt cagatacata gatacaattc
      421 tattaccccc atccatacaa tgccatctca tttcgatact gttcaactac acgccggcca
      481 agagaaccct ggtgacaatg ctcacagatc cagagctgta ccaatttacg ccaccacttc
      541 ttatgttttc gaaaactcta agcatggttc gcaattgttt ggtctagaag ttccaggtta
      601 cgtctattcc cgtttccaaa acccaaccag taatgttttg gaagaaagaa ttgctgcctt
      661 agaaggtggt gctgctgctt tggctgtttc ctccggtcaa gccgctcaaa cccttgccat
      721 ccaaggtttg gcacacactg gtgacaacat cgtttccact tcttacttat acggtggtac
      781 ttataaccag ttcaaaatct cgttcaaaag atttggtatc gaggctagat ttgttgaagg
      841 tgacaatcca gaagaattcg aaaaggtctt tgatgaaaga accaaggctg tttatttgga
      901 aaccattggt aatccaaagt acaatgttcc ggattttgaa aaaattgttg caattgctca
      961 caaacacggt attccagttg tcgttgacaa cacatttggt gccggtggtt acttctgtca
     1021 gccaattaaa tacggtgctg atattgtaac acattctgct accaaatgga ttggtggtca
     1081 tggtactact atcggtggta ttattgttga ctctggtaag ttcccatgga aggactaccc
     1141 agaaaagttc cctcaattct ctcaacctgc cgaaggatat cacggtacta tctacaatga
     1201 agcctacggt aacttggcat acatcgttca tgttagaact gaactattaa gagatttggg
     1261 tccattgatg aacccatttg cctctttctt gctactacaa ggtgttgaaa cattatcttt
     1321 gagagctgaa agacacggtg aaaatgcatt gaagttagcc aaatggttag aacaatcccc
     1381 atacgtatct tgggtttcat accctggttt agcatctcat tctcatcatg aaaatgctaa
     1441 gaagtatcta tctaacggtt tcggtggtgt cttatctttc ggtgtaaaag acttaccaaa
     1501 tgccgacaag gaaactgacc cattcgaact ttctggtgct caagttgttg acaatttaaa
     1561 gcttgcctct aacttggcca atgttggtga tgccaagacc ttagtcattg ctccatactt
     1621 cactacccac aaacaattaa atgacaaaga aaagttggca tctggtgtta ccaaggactt
     1681 aattcgtgtc tctgttggta tcgaatttat tgatgacatt attgcagact tccagcaatc
     1741 ttttgaaact gttttcgctg gccaaaaacc atgagtgtgc gtgatgagtt gtaaaattat
     1801 gtataaacct actttctctc acaagtactg cggtgtgaaa taccgcacag atgcgtaagg
     1861 agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     1921 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     1981 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     2041 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     2101 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     2161 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     2221 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     2281 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc
     2341 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     2401 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     2461 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
     2521 cgaattgggt accgggcccc ccctcgaggt cgacggtatc gataagcttg atatcgaatt
     2581 cctgcagcta tattaccctg ttatccctac gtacgctgca ggtcgacggt ggcggttctg
     2641 gcggtggcgg atccggctct gttgagttaa acctgcgtga gaccgagcta tgtcttggtc
     2701 tacccggtgg tgacactgta gcaccagtga caggcaacaa aaggggcttc agcgagactg
     2761 tagatctgaa gctaaactta aacaacgaac cagctaataa ggaaggtagt acaacacatg
     2821 atgtggttac gtttgacagc aaggagaaga gtgcctgtcc caaagatccg gcaaagcccc
     2881 cggcgaaggc ccaggttgta ggttggcctc cggtaaggtc ctatcgtaaa aacgtaatgg
     2941 ttagttgtca gaagtccagc ggaggacctg aagcagcggc gttcgtcaaa gtatctatgg
     3001 atggcgcccc atacttgaga aagatagacc tgcgtatgta taagtcttac gacgagttat
     3061 caaatgcgct atctaacatg ttcagcagtt tcaccatggg caaacatggt ggtgaggagg
     3121 gaatgatcga cttcatgaac gagagaaaac ttatggacct ggtgaatagc tgggattatg
     3181 tccctagcta cgaggataaa gacggggatt ggatgcttgt gggggatgtt ccgtggccga
     3241 tgttcgtcga tacgtgtaag cgtctgcgtc taatgaaggg ctccgatgct attggtttag
     3301 ctccgcgtgc tatggaaaag tgtaagtcca gagctgtatc acccgggata cattatacga
     3361 agttatgcat gctcactagt gactacaagg acgacgatga caagtctagt gactacaagg
     3421 acgacgatga caagtctagt gactacaagg acgacgatga caagtctagt gactacaagg
     3481 acgacgatga caagtctagt gactacaagg acgacgatga caagtctagt gactacaagg
     3541 acgacgatga caagtctaga tgaggcgcgc tagatctgtt tagcttaaga gttcttcttt
     3601 gagatatcga ttgaacgagc tcgaattcat cgatctatat taccctgtta tccctagcgt
     3661 aactatgcca aatgacaata agacacccaa taggtccagc actcccaagt ttactaaaaa
     3721 accagtaacc ccgaatgata aaattcctga aagacgggtg tacaatatgg acttcctctt
     3781 ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt ctccctaaca
     3841 tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca taatgggcta
     3901 aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa ctaatactgt
     3961 agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt ccatttgcca
     4021 tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt ttctctctcc
     4081 cccgttgttg tctcaccata tccgcaatga caaaaaaatg atggaagaca ctaaaggaaa
     4141 aaattaacga caaagacagc accaacagat gtcgttgttc cagagctgat gaggggtatc
     4201 tcgaagcaca cgaaactttt tccttccttc attcacgcac actactctct aatgagcaac
     4261 ggtatacggc cttccttcca gttacttgaa tttgaaataa aaaaaagttt gctgtcttgc
     4321 tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat tgttctcgtt
     4381 ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag catacaatca
     4441 actatctcat atacaactag aatgcccaag aagaaacgta aggtgggttc cacccttacg
     4501 agcggtcaac gtgaccacag agacgttgaa aatgagttat ccagtgcagc ttttagcagt
     4561 caaagtttcg gggttccaga tatgtcattc aaaccggggt gctccagtga cgtcgcagtc
     4621 actgatgcgg gtatgcctag ccaagggtta tggaacaacc agacgcaaag gatgcgtacc
     4681 ttcactaagg tccagaaaag gggctctgtt gggaggagta tcgacatcac caggtatcgt
     4741 gattacgatg agctacgtca tgatctggca tgtatgttcg gcatacaggg gcagttagaa
     4801 gacccttatc gtatggattg gaagttagtt tatgtggatc acgagaacga tatactgttg
     4861 gtgggcgacg acccgtggga ggaatttgta ggttgtgtta agtctatcaa gattctatct
     4921 gctgcagagg tgcagcaaat gagcttggac ggggatcttg gtggagttcc gccccaaacc
     4981 caggcgtgca gtgcttcaga cgatgcgaac gcttggagag gctatcccta cgacgtgcct
     5041 gattatgcgg gtagcggggc cactaacttc agcttgttga agcaggctgg cgatgtggag
     5101 gagaatccag gtccggcggc taccgataaa gcggaattaa ttcccgagcc tccaaaaaag
     5161 aagagaaagg tcgaattggg taccgccacg tacttcccgg aggaggtggt ggagcacatc
     5221 ttcagcttcc tgccggcgca gcgcgaccgc aacacggtct cgctcgtctg caaggtgtgg
     5281 tacgagatcg agaggctgag ccgccgcggc gtcttcgtgg gcaactgcta cgccgtgcgc
     5341 gccggccgcg tcgccgcgcg gttccccaac gtgcgggcgc tcacggtgaa ggggaagccc
     5401 cacttcgccg acttcaacct cgtgcccccc gactggggcg gctacgcggg gccgtggatc
     5461 gaggcggccg cgaggggatg ccacggcctg gaggagctca ggatgaagcg gatggtggtg
     5521 tccgacgaga gcctcgagct gctggctcgc tcgttcccgc ggttcagggc tcttgttctt
     5581 atcagctgcg aggggttcag cactgacggg ctagccgccg tcgcgagcca ttgcaagctt
     5641 ctgagggagt tggatttgca ggaaaatgaa gtggaggatc gagggcctag gtggctttcc
     5701 tgcttccctg attcctgcac atcacttgtc tcattgaatt ttgcctgcat caaaggggag
     5761 gttaatgctg gttcactgga gagacttgtt agcaggtccc caaacctgcg gagtttgagg
     5821 ctgaatcgat ctgtatcggt agatacactt gcaaagatac tactgcgtac ccctaacttg
     5881 gaggatttgg ggacagggaa tttgacagat gacttccaaa ctgagtccta ctttaagctt
     5941 accagtgctc tggagaaatg caagatgttg aggagtttgt ctggattctg ggatgcttct
     6001 cctgtttgcc tgtcatttat ctaccccctg tgtgctcaac tgacaggatt gaacttgagc
     6061 tatgcaccca cacttgatgc ttctgacctt acaaaaatga ttagccgctg tgtgaagctc
     6121 caacgccttt gggtactgga ttgtatctcg gacaaaggct tgcaagtggt ggcctccagt
     6181 tgcaaagact tgcaagaact cagggtattt ccatcagatt tctacgtagc tggttattct
     6241 gcagtgacag aggagggact tgttgcagta tccttgggct gtccaaaact gaactcacta
     6301 ctgtacttct gtcaccaaat gactaatgct gcactagtta ctgtcgccaa gaactgtcca
     6361 aatttcacac gattcagact ttgtattctt gagccaggga agcctgatgt tgtgacaagc
     6421 caaccattag atgaaggctt tggagctatt gttcgtgagt gcaagggatt acaacgtttg
     6481 tcaatatctg gtcttctcac agacaaagtt ttcatgtata ttgggaaata tgcaaaacaa
     6541 cttgagatgc tttctatagc atttgctggt gacagtgata agggtatgat gcatgttatg
     6601 aatggatgca agaatttaag gaaactggag ataagagata gcccgtttgg tgatgctgca
     6661 ctcttgggga attttgctag gtacgagaca atgcgatccc tttggatgtc atcttgcaat
     6721 gtcacgttaa aggggtgcca agtccttgcg tcaaagatgc cgatgctcaa tgttgaggtc
     6781 ataaatgagc gggatggtag caatgaaatg gaggaaaacc atggagatct gcctaaagtg
     6841 gagaaattat atgtgtaccg cacaactgct ggggcgaggg atgatgcacc aaattttgtt
     6901 aaaatcctag tcgactccgg ttctgctgct agtggttgaa cgcgtggcgc ctggaaaaag
     6961 attttcaagt gaataaggag ataaaaccgt attgactttt tatcattcta caaaagacca
     7021 gtaacgaagt gtaccctgct atattaccct gttatcccta gcgtaactcg gccgccaccg
     7081 cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
     7141 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     7201 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     7261 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     7321 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     7381 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7441 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7501 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7561 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7621 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7681 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7741 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7801 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7861 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7921 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7981 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     8041 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     8101 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     8161 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     8221 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     8281 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     8341 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     8401 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     8461 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     8521 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     8581 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     8641 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8701 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8761 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8821 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8881 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8941 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     9001 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     9061 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     9121 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     9181 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     9241 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcc
     9301 ttttcatcac gtgctataaa aataattata atttaaattt tttaatataa atatataaat
     9361 taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt ccgaagatgt
     9421 aaaagactct agggggatcg ccaacaaata ctacctttta tcttgctctt cctgctctca
     9481 ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag accacacacg aaaatcctgt
     9541 gattttacat tttacttatc gttaatcgaa tgtatatcta tttaatctgc ttttcttgtc
     9601 taataaatat atatgtaaag tacgcttttt gttgaaattt tttaaacctt tgtttatttt
     9661 tttttcttca ttccgtaact cttctacctt ctttatttac tttctaaaat ccaaatacaa
     9721 aacataaaaa taaataaaca cagagtaaat tcccaaatta ttccatcatt aaaagatacg
     9781 aggcgcgtgt aagttacagg caagcgatcc gtctaagaaa ccattattat catgacatta
     9841 acctataaaa ataggcgtat cacgaggccc tttcgtc
//
