LOCUS       sequence-36703-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Koc EC, Spremulli LL
  TITLE     Identification of mammalian mitochondrial translational initiation 
            factor 3 and examination of its role in initiation complex formation
            with natural mRNAs.
  JOURNAL   J Biol Chem. 2002 Sep 20. 277(38):35541-9.
  PUBMED    12095986
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2002 Sep 20. 277(38):35541-9."
COMMENT     Sequence Label: pET21c-IF3mt
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             792..809
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(862..880)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tctagaataa ttttgtttaa ctttaagaag gagatataca tatgacagca ccagcacagt
       61 tgtcccctat tgcttctgcc ccaagactct ccttcctaat tcatgcaaaa gcctttagta
      121 ccgctgaaga cacccagaat gaaggaaaaa agacaaaaaa gaataaaaca gcttttagta
      181 acgttggaag aaaaattagt cagcgagtta ttcacttatt tgatgagaag ggcaatgatt
      241 tgggaaacat gcaccgagca aatgtgatta gacttatgga tgagcgagac ctgcgactgg
      301 ttcaaaggaa caccagcaca gaacctgcag agtatcagct catgacagga ttgcagatcc
      361 tccaggagcg gcagaggctg agggagatgg agaaggcgaa ccccaaaact ggaccaaccc
      421 tgagaaagga actgattttg tcttcaaata ttggacaaca tgatttggac acaaagacta
      481 aacagattca gcagtggatt aagaaaaaac acctagtcca gattaccata aagaaaggaa
      541 aaaatgtaga cgtgtcagaa aatgaaatgg aggagatatt tcatcaaata ctccagacta
      601 tgcctggaat agctacattc tcatctaggc cacaagctgt tcaaggagga aaagctttaa
      661 tgtgtgttct tcgtgctttg agcaaaaatg aggagaaggc atataaagaa actcaagaga
      721 cccaggaaag agacactttg aacaaagacc atggaaatga taaggaatca aatgttctgc
      781 atcagctcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg
      841 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
      901 aacgggt
//
