LOCUS       sequence-36705-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Bullard JM, Cai YC, Spremulli LL
  TITLE     Expression and characterization of the human mitochondrial 
            leucyl-tRNA synthetase.
  JOURNAL   Biochim Biophys Acta. 2000 Feb 29. 1490(3):245-58.
  PUBMED    10684970
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta. 2000 Feb 29. 1490(3):245-58."
COMMENT     Sequence Label: pET21c(+)-mtLeuRS
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cctctagaaa taattttgtt taactttaag aaggagatat acatatgcta aatggtgggc
       61 cagatgtcat caagtgggaa aggagagtaa ttcccggatg taccagaagc atctacagtg
      121 ccacgggaaa gtggacaaaa gagtatacat tgcagacaag aaaggatgtt gagaaatggt
      181 ggcatcaacg aataaaagaa caggcctcca aaatttcaga agctgataaa tcgaagccaa
      241 aattttacgt gctttccatg ttcccttatc cttctggtaa gctgcacatg ggccatgtgc
      301 gtgtctacac catcagcgac accatagcac ggttccagaa gatgagaggg atgcaggtca
      361 tcaaccccat gggatgggat gcttttggat tgcctgctga aaatgccgca gtcgagagga
      421 atctacatcc acaaagttgg acacaaagta atattaaaca catgaggaaa cagcttgatc
      481 gtctgggcct gtgtttcagc tgggataggg aaataactac gtgtttgcca gattactaca
      541 agtggactca gtatctcttt attaaactgt atgaggctgg gctggcctat caaaaggagg
      601 ccctggttaa ctgggaccca gtggatcaaa cagtgcttgc caatgagcag gtggatgaac
      661 atggctgttc atggcgttct ggagcaaagg tggaacagaa gtacctcaga caatggttta
      721 ttaagacaac cgcttatgca aaggccatgc aggacgcgtt ggcagacctt ccagaatggt
      781 atggaataaa aggcatgcaa gcccactgga ttggggactg tgtgggctgc cacctggact
      841 tcacattaaa ggttcatggg caagccacgg gcgaaaagct gactgcctat acggccaccc
      901 ctgaagccat ttatggcacc tcccacgtgg cc
//
