LOCUS       sequence-36706-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Whitmarsh AJ, Shore P, Sharrocks AD, Davis RJ
  TITLE     Integration of MAP kinase signal transduction pathways at the serum 
            response element.
  JOURNAL   Science. 1995 Jul 21. 269(5222):403-7.
  PUBMED    7618106
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1995 Jul 21. 269(5222):403-7."
COMMENT     Sequence Label: pCDNA3 Flag MKK4(ala)
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     58..77
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        58..76
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             121..144
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgnggcttta gcgactctct ggctactgag acccactgct tactggctta tcgaaattaa
       61 tacgactcac tatagggaga cccaagcttg gtaccgagct cggatccggc agccaacatg
      121 gactataagg acgatgacga caaacagggt aaacgcaaag cactgaagtt gaattttgca
      181 aatccacctt tcaaatctac agcaaggttt actctgaatc ccaatcctac aggagttcaa
      241 aacccacaca tagagagact gagaacacac agcattgagt catcaggaaa actgaagatc
      301 tcccctgaac aacactggga tttcactgca gaggacttga aagaccttgg agaaattgga
      361 cgaggagctt atggttctgt caacaaaatg gtccacaaac caagtgggca aataatggca
      421 gttaaaagaa ttcggtcaac agtggatgaa aaagaacaaa aacaacttct tatggatttg
      481 gatgtagtaa tgcggagtag tgattgccca tacattgttc agttttatgg tgcactcttc
      541 agagagggtg actgttggat ctgtatggaa ctcatgtcta cctcgtttga taagttttac
      601 aaatatgtat atagtgtatt agatgatgtt attccagaag aaattttagg caaaatcact
      661 ttagcaactg tgaaagcact aaaccactta aaagaaaact tgaaaattat tcacagagat
      721 atcaaacctt ccaatattct tctggacaga agtggaaata ttaagctctg tgacttcggc
      781 atcagtggac agcttgtgga cgctattgcc aaggcaagag atgctggctg taggccatac
      841 atggcacctg aaagaataga cccaagcgca tcacgacaag gatatgatgt ccgctctgat
      901 gtctggagtt tggggatcac attgtatgag ttg
//
