LOCUS       sequence-36708-e        9153 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Live imaging of apoptotic cells in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9153)
  AUTHORS   van Ham TJ, Mapes J, Kokel D, Peterson RT
  TITLE     Live imaging of apoptotic cells in zebrafish.
  JOURNAL   FASEB J. 2010 Nov;24(11):4336-42. Epub 2010 Jul 2.
  PUBMED    20601526
REFERENCE   2  (bases 1 to 9153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J."; 
            date: "2010-11"; volume: "24(11)"; pages: "4336-42. Epub 2010 Jul 2"
COMMENT     Sequence Label: pBH-UAS-secA5-YFP
FEATURES             Location/Qualifiers
     source          1..9153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(561..572,573..735)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    1554..1578
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2599..2623)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2637..2646
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2643..2645,2646..2648,2649..3362)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2688..2709)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2949..2968)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3296..3317
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3407..3541
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3431..3450)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3485..3504
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3677..4387)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3955..3974)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4224..4242
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    join(6683..6870,6871..6882)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(6916..6932)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6916..6932)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6969..6989)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6969..6987)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7008..7024)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7008..7024)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7021..7043)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7032..7048
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7056..7062,7063..7080,7081..7086))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7101..7122
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7239..7256)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7410..7998)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7490..7509)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8169..8960,8961..9029))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8792..8811
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9030..9134)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggccac aatgacggct actacatggt
      361 gccattcctt cctctttata ggaatggaga ctacctcctg tccaacaagg ctcttggata
      421 cgagtacgcc tacctgttgg acccaggtca ttgcacaaca ccagaaatgc cctctgatct
      481 gcaaaagacg tgaatatctg ttcagacacc catatccact ctgttccaca caggtcagag
      541 gtttgtccag gagttcttga cagaggtgta aaaagtactc aaaaatttta ctcaagtgaa
      601 agtacaagta cttagggaaa attttactca attaaaagta aaagtatctg gctagaatct
      661 tacttgagta aaagtaaaaa agtactccat taaaattgta cttgagtatt aaggaagtaa
      721 aagtaaaagc aagaaagaaa actagagatt cttgtttaag cttttaatct caaaaaacat
      781 taaatgaaat gcatacaagg ttttatcctg ctttagaact gtttgtattt aattatcaaa
      841 ctataagaca gacaatctaa tgccagtaca cgctactcaa agttgtaaaa cctcagattt
      901 aacttcagta gaagctgatt ctcaaaattg ttagtgtcaa gcctagctct tttggggctg
      961 aaaagcaatc ctgcagtgct gaaaagcctc tcacaggcag ccgatgcggg aagaggtgta
     1021 ttagtcttga tagagaggct gcaaatagca ggaaacgtga gcagagactc cctggtgtct
     1081 gaaacacagg ccagatgggc ccccaaggcg gagtactgtc ctccgggctg gcggagtact
     1141 gtcctccggc aaggtcggag tactgtcctc cgacactaga ggtcggagta ctgtcctccg
     1201 acgcaaggcg gagtactgtc ctccgggctg cggagtactg tcctccggca aggtcggagt
     1261 actgtcctcc gacactagag gtcggagtac tgtcctccga cgcaaggtcg gagtactgtc
     1321 ctccgacact agaggtcgga gtactgtcct ccgacgcaag gtcggagtac tgtcctccga
     1381 cactagaggt cggagtactg tcctccgacg caaggcggag tactgtcctc cgggctggcg
     1441 gagtactgtc ctccggcaag ggtcgactct agagggtata taatggatcc catcgcgtct
     1501 cagcctcact ttgagctcct ccacacgaat tcgctagcgg gcccgatgat atcacaagtt
     1561 tgtacaaaaa agcaggctta accatgcata aggttttgct ggcactgttc tttatctttc
     1621 tggcaccagc aggcaccatg gcacaggttc tcagaggcac tgtgactgac ttccctggat
     1681 ttgatgagcg ggctgatgca gaaactcttc ggaaggctat gaaaggcttg ggcacagatg
     1741 aggagagcat cctgactctg ttgacatccc gaagtaatgc tcagcgccag gaaatctctg
     1801 cagcttttaa gactctgttt ggcagggatc ttctggatga cctgaaatca gaactaactg
     1861 gaaaatttga aaaattaatt gtggctctga tgaaaccctc tcggctttat gatgcttatg
     1921 aactgaaaca tgccttgaag ggagctggaa caaatgaaaa agtactgaca gaaattattg
     1981 cttcaaggac acctgaagaa ctgagagcca tcaaacaagt ttatgaagaa gaatatggct
     2041 caagcctgga agatgacgtg gtgggggaca cttcagggta ctaccagcgg atgttggtgg
     2101 ttctccttca ggctaacaga gaccctgatg ctggaattga tgaagctcaa gttgaacaag
     2161 atgctcaggc tttatttcag gctggagaac ttaaatgggg gacagatgaa gaaaagttta
     2221 tcaccatctt tggaacacga agtgtgtctc atttgagaaa ggtgtttgac aagtacatga
     2281 ctatatcagg atttcaaatt gaggaaacca ttgaccgcga gacttctggc aatttagagc
     2341 aactactcct tgctgttgtg aaatctattc gaagtatacc tgcctacctt gcagagaccc
     2401 tctattatgc tatgaaggga gctgggacag atgatcatac cctcatcaga gtcatggttt
     2461 ccaggagtga gattgatctg tttaacatca ggaaggagtt taggaagaat tttgccacct
     2521 ctctttattc catgattaag ggagatacat ctggggacta taagaaagct cttctgctgc
     2581 tctgtggaga agatgacgac ccagctttct tgtacaaagt ggtgatatca ccggtcgcca
     2641 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     2701 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     2761 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     2821 ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga
     2881 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     2941 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     3001 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     3061 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     3121 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     3181 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     3241 actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     3301 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     3361 aaagcggccg ccaccgcggt ggagctcgaa ttaattcatc gatgatgatc cagacatgat
     3421 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     3481 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     3541 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     3601 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcctc tagatcagat
     3661 ccagagtcgc ggccgcttac ttgtacagct cgtccatgcc gccggtggag tggcggccct
     3721 cggcgcgttc gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg atgtccaact
     3781 tgatgttgac gttgtaggcg ccgggcagct gcacgggctt cttggccttg taggtggtct
     3841 tgacctcagc gtcgtagtgg ccgccgtcct tcagcttcag cctctgcttg atctcgccct
     3901 tcagggcgcc gtcctcgggg tacatccgct cggaggaggc ctcccagccc atggtcttct
     3961 tctgcattac ggggccgtcg gaggggaagt tggtgccgcg cagcttcacc ttgtagatga
     4021 actcgccgtc ctgcagggag gagtcctggg tcacggtcac cacgccgccg tcctcgaagt
     4081 tcatcacgcg ctcccacttg aagccctcgg ggaaggacag cttcaagtag tcggggatgt
     4141 cggcggggtg cttcacgtag gccttggagc cgtacatgaa ctgaggggac aggatgtccc
     4201 aggcgaaggg cagggggcca cccttggtca ccttcagctt ggcggtctgg gtgccctcgt
     4261 aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg gagccctcca
     4321 tgtgcacctt gaagcgcatg aactccttga tgatggccat gttatcctcc tcgcccttgc
     4381 tcaccatcgg tacctgtctg ctttgctgtt ggtctgggct cctgggtcac tgacgtttct
     4441 aatggagtct ttatgtatga ggactcttat catttgttct tctataaagg tctgcagtgt
     4501 ttctgttcgt cccctacatg gacacccaga gcctcctaaa tacaggagcc ctgataactg
     4561 cacaagtgct cagattccag cagggtggaa aatgagataa agtgtgcaga tggggagggg
     4621 gacgtgaatg agagaagctt gagctcgaga tctgtcccga tctttctctt ctgtgctgtc
     4681 aatttagagt agtttttgag gtaatttggg tgcattctct gtaaaaacaa caaataaata
     4741 tttatattgt atgcaataaa tgtaacaatt acacatacac tctctgatta cagcattaaa
     4801 gggttagttc acccaaaaat gaaaagatgt cattaatgac tcaccctcat gtcgttccaa
     4861 gcccgtaaca cctccgttca tcttcggaac acagtttaag atattttaga tttagtccga
     4921 gagctttctg tccctccact gaaaatgtat gtacggtata ctgtccatgt ccagaaaggt
     4981 aataaaaaca tcaaagtagt ccatgtgaca tcagtgggtt aggtagaatt atttgaagca
     5041 tcgaaaatac attttggtcc aaaaataaca aaacctacga ctttattcag cattgtagcg
     5101 tcactgcgaa gtcgtgaacg cggattgaca acagacccgg aagagaatac aatgccgaat
     5161 aaagtcgtag gttttgttat ttttggacca aaatgtattc gatgcttcaa aaaattctaa
     5221 ctaacccact gatgtcacat ggactacttt gatgttttta ttacctttct ggacatggac
     5281 agtataccgt acatacattc tcaatggagg cacagaaagc tctcggacta aatctaaaat
     5341 atcttaaact gtgttctgaa gatgaacgga ggtcttacgg gcttggaacg acatgagggc
     5401 gagtcattaa tgacattatt ttcatttttg ggtgaactaa ccctttaata aaactgacat
     5461 ttgcagccaa ttgcacactt tataatgtta aatacttgtt gattttagaa atacagtagc
     5521 atttagtgac aatcaaacat taattttatt tctgattata ttgacaaaaa cacaggactg
     5581 taaggctgac atgacagggt gaattgattt cattacatta gttttgatgg taacactttt
     5641 acaataaggt tcgttagtta actacattag ttaacatgaa ctaataatga actgcacgta
     5701 tacatgttaa tttcaacatt tactaatact ttatatcttg ttaacattag ttaatgcact
     5761 gtgaactaac atgaacaaac aatgaacatc tgtatttcta ttaactaacg ttaacaaaga
     5821 ttaacagata cagtaacaaa tgtattgctc atggttattt aatgttagtt aatacatgaa
     5881 ctaatcaacc tcattgtaaa gtgttaccgt tttgacaata caacatgttt ggaaaaaaat
     5941 ttctcgcttg gttttcttgt gttttgcatg aattcaaaca caatgagagc acggaatgat
     6001 aatgattatt caaagctgca ataggcgcca aatttcccgt ggacctaacg ttaccaatta
     6061 caaatgcatt ttagcatttt attacgtgag ccttatattg tcaatcgcat gcattagtga
     6121 aaataggcct actacacata taaaaggcgc gccatcaacg tcaaaacatg cacacccaaa
     6181 aaaaaaaaaa aaaaaaaaaa aaaaaactat cagtaaaaca aaatacttaa tgtacttacc
     6241 tcaatatgct tccttaggtt tgatggcgaa cttttgaagg ccgatatttc tttattaagc
     6301 gggagacaga ggacacattt catcttgaat gaatctttat ttacaccact taaagaaaag
     6361 aattcgcgaa gatacggcca cgggtgctct tgatcctgtg gctgattttg gactgtgctg
     6421 ctcgcagctg ctgatgaatc acatacttcc tccattttct tccactgatt gactgttata
     6481 atttccctaa tttccaggtc aaggtgctgt gcattgtggt aatagatgtg acatgacgtc
     6541 acttccaaag gaccaatgaa catgtctgac caatttcata taatgtgaaa acgattttca
     6601 taggcagaat aaataacatt taaattaaac tgggcatcag cgcaattcaa ttggtttggt
     6661 aatagcaagg gaaaatagaa tgaagtgatc tccaaaaaat aagtactttt tgactgtaaa
     6721 taaaattgta aggagtaaaa agtacttttt tttctaaaaa aatgtaatta agtaaaagta
     6781 aaagtattga tttttaattg tactcaagta aagtaaaaat ccccaaaaat aatacttaag
     6841 tacagtaatc aagtaaaatt actcaagtac tttacacctc tggttcttga ccccctacct
     6901 tcagcaagcc cagcagatcc actagttcta gagcggccgc caccgcggtg gagctccagc
     6961 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
     7021 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     7081 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     7141 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     7201 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     7261 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     7321 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7381 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7441 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7501 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7561 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7621 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7681 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7741 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7801 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     7861 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7921 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7981 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8041 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8101 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     8161 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     8221 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     8281 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     8341 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8401 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8461 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8521 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8581 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8641 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8701 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8761 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8821 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8881 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8941 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     9001 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9061 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     9121 ataggggttc cgcgcacatt tccccgaaaa gtg
//
