LOCUS       sequence-367109-        8322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  KSHV anti-RTA_3Kb_Reverse.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8322)
  TITLE     Dittmer lab plasmids Jan 2022
REFERENCE   2  (bases 1 to 8322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pOME0294M
FEATURES             Location/Qualifiers
     source          1..8322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3913..3930)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3919..4143
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4189..4617
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4276..4295)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4486..4507
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4626..4646)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4631..4960
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4811..4946
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4873..4892
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5027..5821
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5081..5100)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5691..5710
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5995..6116
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6032..6051)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6086..6105
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6165..6181)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6165..6181)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6178..6200)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6189..6205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6213..6219,6220..6237,6238..6243))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6258..6279
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6396..6413)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6567..7155)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6647..6666)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7326..8117,8118..8186))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7949..7968
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8187..8291)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga ctcgagcggc cgcactgttc catttctagc tggaaaaata
      961 acatatgact tgttacgcac aaatgttacc acatccaccc tcgtctcaag cggttcgaga
     1021 tgcaggcttg tcagcccaca gtcccgagaa taattcacaa gccacatgaa ctggtttcgg
     1081 cgccttggat ccaacccaga catgtccagc atggtccgtt ttttcctagc ccgccacgaa
     1141 aagtcctctc aatccctgca cgactaatac acctgttcga agacgagcat ttacgtggcg
     1201 accatgtaga cttgctgtcg acaggttttt tttgtttcca agtcgacgtg caattttaga
     1261 cataggactt tcaggagcat ttactgtcaa agatggttct ttttgaaaaa atggtgcgac
     1321 cggtaggtga ctttcctccc tcgtgtgcag ggcaacacgc aacaatccta tagggattga
     1381 agcctcgcct ctgccggcct gcgagggtgt tttaccctct ccgtggtgag acacgtcagg
     1441 cgccagcccc caagactaag gtccccgcgg cacaccccca taacctctca gttttgagac
     1501 ccgtcaggga attactcgag agagagtttt ggatacgtcg gtcgcaggtg atcaccgtcg
     1561 tacggcaatt attgtgggga atagaacagc aacggttcaa acatgttgac gacgtccctt
     1621 attcggttta agcgggatcg gcgcccttgg tccatgccga gcgaaacagc cacgacctgg
     1681 ttatagactt accagaaacg ttccatatcc cagaagagtt gcaaatctcg cccatgcacc
     1741 gtcagaccta actcccaccg ctgcctgtcc catagattga ggacttcata gactagggtc
     1801 ctgcccatta ctatggattt gtctaccaac ttgtccacta gaaattcccc ggaagagcta
     1861 cagtaacatt tttgatactg tgcggtgaga gaggaatccc attcttcgaa gccgccagga
     1921 cacacctttc gaagcagccg gagagcctgc ttgacttccg ggttgagatg gtcacacacg
     1981 aggaatattt actgcgtatg ctttgttaga tgctagggtc actggattta tctcaccacc
     2041 ttctacacac gtaagcctaa tactttctta caaggttcga accacgcgat acaccagaca
     2101 aatgtctgta attgtacaaa ttggaaaaga cgaaaaaagc acggagagct tactcctggt
     2161 ttccgcgccg gcccatgttg cacggtacgc gtctccgtag ggttccgtaa taagcctagg
     2221 agtgcctctc ctagaataag acgtgtcttt tccgtaaaga ctgtcggcgt acgtcgcccc
     2281 actcggacgg aggtcggtat acattcgatg atgtgcttta tatgtgactt tacttccggt
     2341 ttacggaccc ccggacctcc gctgagcaga cgttagcctc cgggtaatac tataattgga
     2401 gaagggatga cttcgagatg ttgtgcatgc tatggcggcc cgacgagaga ctcgtcaggt
     2461 cccgggagac ggaaaaccaa aaggttggcc agatggaagg ctcctaatac cgcggcgacc
     2521 tctagtactg gttcccagtc gagcggggac ttttgaaaat gtcgtagtgg ccaagacgac
     2581 tctttgcggc cggttaatgg tggccgttcc agtgacctga caggataggt ccttcgccag
     2641 agtacggtct tagaaattaa aacggttagg acctcggtcc tgacaacggc cgaaggtacc
     2701 atctggagtc gctacacgac cgttttgggc ggcaataaga ctcgcgggga cgggactcgg
     2761 tcaaacagta atcgtttggg gtagggttgt acggcgtgtg gcagtcgtag taggggaaat
     2821 tgggtagccc atgtctgggc cgcaaataat catgcaccgt ccggcgcagt gtcttatacc
     2881 acatgttgtg taggtggcgc gggaattttg gccggtggcc atcaagtgtc tgccacagtc
     2941 agttccgcca acgagttccc cggcactaat gacgctgttg ccacggcgtc cgttacggtc
     3001 gcgccccatg gcctcccctc aacggacatt acagtcgcag gtgaggacgt tctctagtcc
     3061 agcgacgtac aaaacagcgt ctcttgtggc ctctaagagg gctgttgggc tcgagaaagg
     3121 actgcagtac agtgggaacg ctaggcttgt gccaatatca ccgggtcgtt aaagttggtg
     3181 gcgttacgca atgcaacaac gtccaatgga cacgggggag aagctgtggt ggggggctaa
     3241 gttgtcaggc ccggggccac cacgtcaacg gttgtcatca gggagacggc cggtcgcgca
     3301 aggagggccg cgagcgggtt ggtcttcgga gcccgcttct cgaaggcccg ccagtactgc
     3361 ctctggttcc acacggcaca tctctaagtt gccgccgccg atgccgccgt ctccgctgtt
     3421 gtgggtttgc tttcgtctct tcgtttctct cctcgagttt cttcgcattc cgaaactggc
     3481 acggtcttcg gctgtggtgc ggtagctggt gctgtggacc atggagaaac cctagttaat
     3541 ggtggggggt cctacacgtg cggtgcctac agcggtgcag actccctggt agcctccgtg
     3601 ttgggggcga tgacagcaat gggggcggtg acctgcatct agtctcagat aagcgggaca
     3661 atctgcttcg cccgggactt tgtaccctac agcccagcgg agagaggggg tgactgctgc
     3721 gcgacaacag gtcataagac gttcctgaca tggtcgacct gtgcggtggc ggagacgcca
     3781 gtgggagggg gcgaaggaag ccgggcctca gaggccgcct atatggcagt ggaagaccac
     3841 ctctcgaatt ccaccacact ggactagtgg atccgagctc ggtaccaagc ttaagtttaa
     3901 accgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     3961 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     4021 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     4081 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     4141 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     4201 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     4261 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     4321 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     4381 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     4441 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     4501 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     4561 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     4621 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     4681 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     4741 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     4801 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4861 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     4921 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     4981 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     5041 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     5101 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     5161 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     5221 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     5281 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     5341 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     5401 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     5461 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     5521 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     5581 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     5641 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     5701 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     5761 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     5821 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     5881 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     5941 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     6001 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6061 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6121 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     6181 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     6241 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6301 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     6361 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     6421 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     6481 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6541 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6601 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6661 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6721 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6781 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6841 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6901 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6961 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     7021 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7081 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7141 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7201 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7261 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7321 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7381 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7441 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7501 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7561 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7621 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7681 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7741 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7801 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7861 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7921 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7981 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8041 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8101 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8161 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8221 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8281 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
