LOCUS       sequence-367129-        8246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses nuclear-localised EGFP under EF1a promoter. For insertion 
            of genetic barcodes into 3'UTR of EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8246)
  AUTHORS   Ratz M, von Berlin L, Larsson L, Martin M, Westholm JO, La Manno G, 
            Lundeberg J, Frisen J
  TITLE     Clonal relations in the mouse brain revealed by single-cell and 
            spatial transcriptomics.
  JOURNAL   Nat Neurosci. 2022 Mar;25(3):285-294. doi: 
            10.1038/s41593-022-01011-x. Epub 2022 Feb 24.
  PUBMED    35210624
REFERENCE   2  (bases 1 to 8246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-022-01011-x"; journalName: "Nat Neurosci"; date: 
            "2022-03"; volume: "25"; issue: "3"; pages: "285-294"
COMMENT     Sequence Label: pMR506-EF1a-H2B-EGFP
FEATURES             Location/Qualifiers
     source          1..8246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    133..154
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(169..174,175..192,193..199)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    207..223
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     212..234
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     231..247
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     231..247
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     266..286
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        268..286
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        314..540
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             541..721
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    768..893
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1386..1619
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1804..1848
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2146..2394
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2146..2166
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2317..2336
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    2490..2607
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2675..3853
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2906..3844
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3801..3821
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3884..4261
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      4274..4283
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4280..4282,4283..4285,4286..4999)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4280..4282,4283..4285,4286..4996)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4325..4346)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4586..4605)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4933..4954
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5078..5311
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5383..5504
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5420..5439)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5474..5493
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5544..5679
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5699..5718)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5700..5718)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5728..5745)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5728..5744)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5737..5759)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5886..6341
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5973..5992)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6183..6204
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6367..6471
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6472..6540,6541..7332)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6690..6709)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7503..8091
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7992..8011
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(8245..8246,1..16)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
       61 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
      121 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
      181 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
      241 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
      301 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
      361 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
      421 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
      481 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
      541 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
      601 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      661 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      721 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      781 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      841 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      901 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      961 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     1021 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     1081 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     1141 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     1201 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     1261 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1321 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1381 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1441 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1501 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1561 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1621 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1681 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1741 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1801 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1861 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1921 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1981 ctattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     2041 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     2101 cggtatcgat cacgagacta gcctcgagcg gccgccccct tcaccgaggg cctatttccc
     2161 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tggaattaat
     2221 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
     2281 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
     2341 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccggcaac
     2401 aagatgaaga gcaccaactc gagttggtgc tcttcatctt gttgttttta attctcgacc
     2461 tcgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     2521 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     2581 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt
     2641 tggactagtc taggtcttga aaggagtggg aattggctcc ggtgcccgtc agtgggcaga
     2701 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc
     2761 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     2821 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     2881 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct
     2941 ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat
     3001 tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg
     3061 agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg
     3121 aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa
     3181 tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca
     3241 agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc
     3301 ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg
     3361 gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc
     3421 gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct
     3481 tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg
     3541 tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc
     3601 cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc
     3661 gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga
     3721 gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga
     3781 gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt
     3841 tcaggtgtcg tgacgtacgg ccaccatgag cgtcgacgcc accatgccag agccagcgaa
     3901 gtctgctccc gccccgaaaa agggctccaa gaaggcggtg actaaggcgc agaagaaagg
     3961 cggcaagaag cgcaagcgca gccgcaagga gagctattcc atctatgtgt acaaggttct
     4021 gaagcaggtc caccctgaca ccggcatttc gtccaaggcc atgggcatca tgaactcgtt
     4081 tgtgaacgac attttcgagc gcatcgcagg tgaggcttcc cgcctggcgc attacaacaa
     4141 gcgctcgacc atcacctcca gggagatcca gacggccgtg cgcctgctgc tgcctgggga
     4201 gttggccaag cacgccgtgt ccgagggtac taaggccatc accaagtaca ccagcgctaa
     4261 ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     4321 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     4381 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     4441 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     4501 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     4561 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     4621 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     4681 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     4741 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     4801 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     4861 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     4921 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     4981 ggacgagctg tacaagtaaa tgcatcggta cctttaagac caatgactta caaggcagct
     5041 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     5101 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     5161 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     5221 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     5281 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     5341 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     5401 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     5461 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     5521 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     5581 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     5641 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
     5701 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     5761 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     5821 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     5881 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     5941 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     6001 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     6061 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     6121 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     6181 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     6241 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     6301 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     6361 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6421 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     6481 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     6541 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     6601 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     6661 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     6721 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     6781 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     6841 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6901 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6961 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     7021 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     7081 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     7141 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     7201 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     7261 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     7321 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     7381 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     7441 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7501 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7561 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7621 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     7681 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7741 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7801 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7861 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7921 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7981 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     8041 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     8101 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     8161 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     8221 cgccgcagcc gaacgaccga gcgcag
//
