LOCUS       sequence-367136-        9329 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NDUFA10 subunit of Complex I fused with C-terminal FKBP and HA 
            epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9329)
  AUTHORS   Balderas E, Eberhardt DR, Lee S, Pleinis JM, Sommakia S, Balynas AM,
            Yin X, Parker MC, Maguire CT, Cho S, Szulik MW, Bakhtina A, Bia RD, 
            Friederich MW, Locke TM, Van Hove JLK, Drakos SG, Sancak Y, 
            Tristani-Firouzi M, Franklin S, Rodan AR, Chaudhuri D
  TITLE     Mitochondrial calcium uniporter stabilization preserves energetic 
            homeostasis during Complex I impairment.
  JOURNAL   Nat Commun. 2022 May 19;13(1):2769. doi: 10.1038/s41467-022-30236-4.
  PUBMED    35589699
REFERENCE   2  (bases 1 to 9329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-05-19"; volume: "13(1):2769. doi"; pages: " 
            10.1038/s41467-022-30236-4"
COMMENT     Sequence Label: pLenti-NDUFA10-FKBP-HA
FEATURES             Location/Qualifiers
     source          1..9329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     32..52
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     78..102
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      158..167
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1108..1122
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
     CDS             1253..1573
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             1586..1612
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    1622..2210
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1675..1695)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2093..2104)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        2224..2723
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2259..2282)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2656..2675
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2744..3343
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2744..2763)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3240..3260
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3481..3714
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3786..3907
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3823..3842)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3877..3896
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3947..4082
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4102..4121)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4103..4121)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4131..4148)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4131..4147)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4140..4162)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4289..4744
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4376..4395)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4586..4607
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4770..4874
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4875..4943,4944..5735)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5093..5112)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5906..6494
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6395..6414
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6648..6665
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6782..6803
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6818..6823,6824..6841,6842..6848)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6856..6872
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6861..6883
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6880..6896
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6880..6896
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6915..6935
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6917..6935
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6963..7189
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7190..7370
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7417..7542
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8035..8268
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8453..8497
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8764..8880
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        8903..9206
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(9207..9329,1..81)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
       61 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
      121 cgctgttttg acctccatag aagacaccga ctctagagcc accatggcct tgcggctcct
      181 gaagctggca gcgacgtccg cgtccgcccg ggtcgtggcg gcgggcgccc agcgcgtgag
      241 aggaattcat agcagtgtgc agtgcaagct gcgctatgga atgtggcatt tcctacttgg
      301 ggataaagca agcaaaagac tgacagaacg cagcagagtg ataactgtag atggcaatat
      361 atgtactgga aaaggcaaac ttgcaaaaga aatagcagag aaactaggct tcaagcactt
      421 tcctgaagcg gggattcatt atccagacag taccacagga gatgggaagc ccctcgccac
      481 cgactataat ggcaactgta gtttggagaa attttacgat gatccgagaa gcaatgatgg
      541 caacagttac cgcctgcagt cctggttgta cagcagtcgc ctgctgcagt actcagatgc
      601 cttggagcac ttgctgacca caggacaagg tgttgtgttg gagcgctcca tcttcagtga
      661 ctttgtgttc ctggaggcga tgtacaacca gggattcatc cgaaagcagt gtgtggacca
      721 ctacaacgag gtgaagagcg tcaccatctg cgattacctg cccccccacc tggtgattta
      781 catcgatgtg cccgttccag aggtccagag gcggattcag aagaaaggag atccacatga
      841 aatgaagatc acctctgcct atctacagga cattgagaat gcctataaga aaacctttct
      901 ccctgagatg agtgaaaaat gtgaggtttt acagtattct gcaagggaag ctcaagattc
      961 aaaaaaggtg gtagaggaca ttgaatacct gaagttcgat aaagggccgt ggctcaagca
     1021 ggacaatcgc actttatacc acctgcgatt actggttcag gataagtttg aggtgctgaa
     1081 ttacacaagc attcctatct ttctcccgga agtcaccatt ggagctcatc agactgaccg
     1141 tgtcttacat cagttcagag agctgccggg ccgcaagtac agccctgggt acaacaccga
     1201 ggtgggagac aagtggatct ggttaaacat tggtgggata tcaggcggtg gaggagtgca
     1261 ggtggaaacc atctccccag gagacgggcg caccttcccc aagcgcggcc agacctgcgt
     1321 ggtgcactac accgggatgc ttgaagatgg aaagaaattt gattcctccc gggacagaaa
     1381 caagcccttt aagtttatgc taggcaagca ggaggtgatc cgaggctggg aagaaggggt
     1441 tgcccagatg agtgtgggtc agagagccaa actgactata tctccagatt atgcctatgg
     1501 tgccactggg cacccaggca tcatcccacc acatgccact ctcgtcttcg atgtggagct
     1561 tctaaaactg gaaggatccg gtggataccc atacgatgtt ccagattacg cttaagtcga
     1621 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     1681 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     1741 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     1801 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     1861 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     1921 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     1981 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     2041 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     2101 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     2161 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattct
     2221 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
     2281 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
     2341 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
     2401 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
     2461 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
     2521 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
     2581 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
     2641 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
     2701 cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc
     2761 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     2821 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     2881 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     2941 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     3001 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     3061 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     3121 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     3181 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     3241 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     3301 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc
     3361 gcagcgcccg accgaaagga gcgcacgacc ccatgcatct cgagggcccg gtacctttaa
     3421 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     3481 tggaagggct agctcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     3541 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     3601 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     3661 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     3721 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     3781 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3841 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     3901 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     3961 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     4021 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4081 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     4141 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     4201 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     4261 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     4321 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4381 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4441 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4501 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4561 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4621 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     4681 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     4741 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4801 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4861 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4921 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4981 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     5041 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     5101 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     5161 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     5221 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     5281 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     5341 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     5401 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     5461 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     5521 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     5581 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     5641 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5701 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5761 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5821 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5881 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5941 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6001 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     6061 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6121 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6181 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6241 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6301 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6361 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6421 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6481 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6541 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     6601 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     6661 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     6721 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     6781 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     6841 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     6901 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     6961 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     7021 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     7081 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     7141 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     7201 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7261 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7321 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     7381 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     7441 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     7501 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     7561 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     7621 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     7681 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     7741 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     7801 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     7861 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     7921 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     7981 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     8041 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     8101 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     8161 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     8221 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     8281 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     8341 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     8401 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     8461 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     8521 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     8581 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     8641 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     8701 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     8761 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     8821 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     8881 atcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     8941 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     9001 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     9061 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     9121 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     9181 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     9241 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     9301 tgttttggca ccaaaatcaa cgggacttt
//
