LOCUS       sequence-367138-       10591 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express C-terminal YC fused influenza A virus partial NS1 protein 
            (27-236 nt) for dsRNA labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10591)
  AUTHORS   Cheng X, Deng P, Cui H, Wang A
  TITLE     Visualizing double-stranded RNA distribution and dynamics in living 
            cells by dsRNA binding-dependent fluorescence complementation.
  JOURNAL   Virology. 2015 Nov;485:439-51. doi: 10.1016/j.virol.2015.08.023. 
            Epub 2015 Sep 7.
  PUBMED    26351203
REFERENCE   2  (bases 1 to 10591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2015-11"; volume: "485:439-51. doi"; pages: " 
            10.1016/j.virol.2015.08.023. Epub 2015 Sep 7"
COMMENT     Sequence Label: pdRBFC-NS1-YC
FEATURES             Location/Qualifiers
     source          1..10591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        2306..2651
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2594..2614
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    2681..2735
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             2740..2763
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    2767..2791
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3008..3032)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     3219..3240
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      3361..4068
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(4093..4110)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4093..4109)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4102..4124)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4312..4336
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5637..6266
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6695..7768
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7834..8028
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8264..8286)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8372..8512
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8427..8446
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8527..8544)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8698..9286)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8778..8797)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9373..10167)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg
     1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata
     1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa
     1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac
     1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa
     1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc
     1681 cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt
     1741 cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac
     1801 cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
     1861 tagagaggcc tacgcagcag gtctcatcaa gacgatctac ccgagtaaca atctccagga
     1921 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat
     1981 caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat
     2041 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt
     2101 tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac
     2161 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga
     2221 aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata
     2281 cagtctcaga agaccaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc
     2341 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     2401 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg
     2461 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     2521 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
     2581 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     2641 tggagaggac acgctcgagt ataagagctc tatttttaca acaattacca acaacaacaa
     2701 acaacaaaca acattacaat tacatttaca attaccatgg actacaaaga cgatgacgac
     2761 aaaatcacaa gtttgtacaa aaaagcaggc ttcatgatgg atccaaacac tgtgtcaagc
     2821 tttcaggtag attgctttct ttggcatgtc cgcaaacgag ttgcagacca agaactaggt
     2881 gatgccccat tccttgatcg gcttcgccga gatcagaaat ccctaagagg aaggggcagc
     2941 accctcggtc tggacatcga gacagccaca cgtgctggaa agcagatagt ggagcggatt
     3001 ctgaaagacc cagctttctt gtacaaagtg gtgcctaggc ctgcttgtaa gatccctaac
     3061 gacctcaagc agaaagtcat gaaccacggc agcgtgcagc tcgccgacca ctaccagcag
     3121 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     3181 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     3241 accgccgccg ggatcactct cggcatggac gagctgtaca agtgacctag gtgagtctag
     3301 agagttaatt ctaggtgagt ctagagagtt aattaagacc cgggactagt ccctagagtc
     3361 ctgctttaat gagatatgcg agacgcctat gatcgcatga tatttgcttt caattctgtt
     3421 gtgcacgttg taaaaaacct gagcatgtgt agctcagatc cttaccgccg gtttcggttc
     3481 attctaatga atatatcacc cgttactatc gtatttttat gaataatatt ctccgttcaa
     3541 tttactgatt gtaccctact acttatatgt acaatattaa aatgaaaaca atatattgtg
     3601 ctgaataggt ttatagcgac atctatgata gagcgccaca ataacaaaca attgcgtttt
     3661 attattacaa atccaatttt aaaaaaagcg gcagaaccgg tcaaacctaa aagactgatt
     3721 acataaatct tattcaaatt tcaaaagtgc cccaggggct agtatctacg acacaccgag
     3781 cggcgaacta ataacgctca ctgaagggaa ctccggttcc ccgccggcgc gcatgggtga
     3841 gattccttga agttgagtat tggccgtccg ctctaccgaa agttacgggc accattcaac
     3901 ccggtccagc acggcggccg ggtaaccgac ttgctgcccc gagaattatg cagcattttt
     3961 ttggtgtatg tgggccccaa atgaagtgca ggtcaaacct tgacagtgac gacaaatcgt
     4021 tgggcgggtc cagggcgaat tttgcgacaa catgtcgagg ctcagcagga cctgcaggca
     4081 tgcaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     4141 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     4201 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgct agagcagctt
     4261 gagcttggat cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat
     4321 atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag
     4381 ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc
     4441 tcgggatcaa agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg
     4501 ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc
     4561 tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa
     4621 tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct
     4681 gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca
     4741 cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc
     4801 ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct
     4861 agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc
     4921 cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg
     4981 catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg
     5041 cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac
     5101 cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt
     5161 gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg
     5221 cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt
     5281 gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa
     5341 ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg
     5401 atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa
     5461 atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa
     5521 gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat
     5581 gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga
     5641 aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc
     5701 tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg
     5761 gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg
     5821 accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg
     5881 acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc
     5941 tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg
     6001 ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg
     6061 cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc
     6121 ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca
     6181 caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg
     6241 ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac
     6301 aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag
     6361 cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac
     6421 caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata
     6481 catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg
     6541 ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca
     6601 tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgcctgc cggccctgca
     6661 atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc
     6721 ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc
     6781 cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc
     6841 tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa
     6901 gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac
     6961 ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg
     7021 agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc
     7081 ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac
     7141 cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc
     7201 acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga
     7261 cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa
     7321 ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa
     7381 gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat
     7441 gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt
     7501 tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa
     7561 ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt
     7621 caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga
     7681 tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat
     7741 cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct
     7801 agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc
     7861 aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa
     7921 ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa
     7981 ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca
     8041 gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc
     8101 cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc
     8161 aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca
     8221 tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     8281 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     8341 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     8401 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     8461 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     8521 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8581 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8641 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8701 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8761 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8821 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8881 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8941 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9001 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9061 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9121 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9181 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9241 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9301 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9361 gcattctagg tactaaaaca attcatccag taaaatataa tattttattt tctcccaatc
     9421 aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc cgatatcctc
     9481 cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc cgcttctccc
     9541 aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt ctcccaggtc
     9601 gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat catacagctc
     9661 gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat cggccagatc
     9721 gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg tatagggaca
     9781 atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct cgataatctt
     9841 ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg cctcactcat
     9901 gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa caggcagctt
     9961 tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg tccctttata
    10021 ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct tatatacctt
    10081 agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca gttttttcaa
    10141 ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct acagtattta
    10201 aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt ccttgcattc
    10261 taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt ggcgtataac
    10321 atagtatcga cggagccgat tttgaaaccg cggtgatcac aggcagcaac gctctgtcat
    10381 cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag
    10441 ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc
    10501 tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga
    10561 gctgccggtc ggggagctgt tggctggctg g
//
