LOCUS       sequence-367139-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-TT8
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     421..437
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     421..437
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     442..461
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        442..460
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(635..1825)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1454..1473
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(1873..1878,1879..1895,1896..1901))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(join(2028..2600,1..16))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2108..2127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
       61 cgacgcgtaa ctcacgttaa gggattttgg tcatgggtgg ctcgaccaga tatggtctcg
      121 aagtaatggg ggtggcaaac aggctcttga gactgttcag cgccttctac cagttctctg
      181 tcaagcccac ggcctgacgc ccgagcaagt tgtagcgatt gctagtaatg ggggtggcaa
      241 acaggctctt gagactgttc agcgccttct accagttctc tgtcaagccc acggcctgac
      301 gcccgagcaa gttgtagcga ttgcttcctg agaccagctt tggtctagag gaggcatgat
      361 gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacggg ccagagctgc
      421 caggaaacag ctatgaccat gtaatacgac tcactatagg ggatatttta ttttctccca
      481 atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatcaa
      541 gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg
      601 gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg
      661 caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca
      721 acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccaatga
      781 tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat
      841 ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa
      901 gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca
      961 gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg
     1021 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc
     1081 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg
     1141 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca
     1201 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atgggtcggc
     1261 gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga
     1321 gcaccgccgc cgcaaggaat ggtgcatgta aggagatggc gcccaacagt cccccggcca
     1381 cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc
     1441 gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg
     1501 tgatgccggc cacgatgcgt ccggcgtaga gaatccacag gacgggtgtg gtcgccatga
     1561 tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc
     1621 ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta
     1681 gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg
     1741 gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga
     1801 cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg
     1861 ataaactacc gcattaaagc taatcgatga taagctgtca aacatgagaa ttaattcatg
     1921 ttctttcctg cgttatcccc tgattctgtg gataacgcag gaaagaacat gtgagcaaaa
     1981 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2041 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2101 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2161 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2221 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2281 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2341 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     2401 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     2461 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2521 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2581 aagcagcaga ttacgcgcag
//
