LOCUS       sequence-36714-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Takeuchi N, Kawakami M, Omori A, Ueda T, Spremulli LL, Watanabe K
  TITLE     Mammalian mitochondrial methionyl-tRNA transformylase from bovine 
            liver. Purification, characterization, and gene structure.
  JOURNAL   J Biol Chem. 1998 Jun 12. 273(24):15085-90.
  PUBMED    9614118
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1998 Jun 12. 273(24):15085-90."
COMMENT     Sequence Label: pET15-MTFmt
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             14..36
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             55..72
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..99
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 cctctagaat aattttgttt aactttaaga aggagatata ccatgggcag cagccatcat
       61 catcatcatc acagcagcgg cctggtgccg cgcggcagcc atatggcgtc ccctggctgg
      121 gaggacggcc agggcgctcg agtccgcgag aagccgcctt ggagggtgct ctttttcggc
      181 aatgaccagt tcgcccgcga aacgctgcgg gcgctgcacg ccgccaggga aaacaaagag
      241 gaagagttaa ttgagaagtt ggaggtggtc acagtgccct ccccatcacc aaaaggactg
      301 ccagtgaagc aatatgctgt ccagtctcag cttccagtgt atgagtggcc agatgtggga
      361 tctggagaat acgatgttgg cgtggtagca tcatttggcc gacttttgag tgaggctttt
      421 attcttaaat ttccctacgg catcttgaat gtccatccca gttgccttcc gaggtggcgt
      481 ggtccagccc ctataatcca caccatcctg catggagaca caattgctgg agtaacgatt
      541 atgcaaatta aacccagaag gtttgatgta ggtccaatcc tcaaacagga gacggttcct
      601 gtgccaccca agagcacctc aaaggaattg gaagcagtgc tgtcaagact gggtgccaac
      661 atgcttattt cagttttgaa aaatttgcct gaaagtttga ataatggaag acagcagcca
      721 gccgaggggg tgacacacgc ccctaagatt tctgctgcta ccagctgtat aaaatgggag
      781 gaacaaactt cagagcagat attcagactt tatcgtgctg ttggaaatat aattccgttg
      841 cagacactct ggatggataa taccattaaa cttctggatt tggtagaagt tgataattca
      901 atcctcagtg attcaaaatt aacaggacaa gctgttattc cagga
//
