LOCUS       sequence-367141-        2602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-TT5
FEATURES             Location/Qualifiers
     source          1..2602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(328..333,334..350,351..356))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(483..1071)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(563..582)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1478..1494
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1478..1494
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1499..1518
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1499..1517
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(1692..2602,1..280))
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     2511..2530
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg
       61 ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca
      121 acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat
      181 cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga
      241 cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt
      301 agcaatttaa ctgtgataaa ctaccgcatt aaagctaatc gatgataagc tgtcaaacat
      361 gagaattaat tcatgttctt tcctgcgtta tcccctgatt ctgtggataa cgcaggaaag
      421 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      481 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      541 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      601 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      661 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      721 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      781 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      841 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      901 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      961 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1021 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1081 ttgatctttt ctacggggtc tgacgctcag tggaacgacg cgtaactcac gttaagggat
     1141 tttggtcatg ggtggctcga ccagatatgg tctcggcaat tgctagtaat gggggtggca
     1201 aacaggctct tgagactgtt cagcgccttc taccagttct ctgtcaagcc cacggcctga
     1261 cgcccgagca agttgtagcg attgctagta atgggggtgg caaacaggct cttgagactg
     1321 ttcagcgcct tctaccagtt ctctgtcaag cccacggcct gacgcccgag caagttgtag
     1381 cgatttgaga ccagctttgg tctagaggag gcatgatgat atatttttat cttgtgcaat
     1441 gtaacatcag agattttgag acacgggcca gagctgccag gaaacagcta tgaccatgta
     1501 atacgactca ctatagggga tattttattt tctcccaatc aggcttgatc cccagtaagt
     1561 caaaaaatag ctcgacatac tgttcttccc cgatcaaggg ttggtttgcg cattcacagt
     1621 tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg
     1681 ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg
     1741 cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag
     1801 gcggcataaa tcgccgtgac gatcagcggt ccaatgatcg aagttaggct ggtaagagcc
     1861 gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc
     1921 tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc
     1981 cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg
     2041 ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg
     2101 agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga
     2161 aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg
     2221 ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag
     2281 ctgactgggt tgaaggctct caagggcatg ggtcggcgct ctcccttatg cgactcctgc
     2341 attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt
     2401 gcatgtaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg
     2461 ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg
     2521 gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg
     2581 gcgtagagaa tccacaggac gg
//
