LOCUS       sequence-36715-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Tournier C, Whitmarsh AJ, Cavanagh J, Barrett T, Davis RJ
  TITLE     The MKK7 gene encodes a group of c-Jun NH2-terminal kinase kinases.
  JOURNAL   Mol Cell Biol. 1999 Feb . 19(2):1569-81.
  PUBMED    9891090
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1999 Feb . 19(2):1569-81."
COMMENT     Sequence Label: pCDNA3 Flag MKK7b2
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     58..77
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        58..76
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             104..127
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(254..265)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 cgnggtttag cgactctctg gctactgaga acccactgct tactggctta tcgaaattaa
       61 tacgactcac tatagggaga cccaagcttc ggcagccaac atggactata aggacgatga
      121 tgacaaaatg gcggcgtcct ccctggagca gaagctgtcc cgcctggaag ccaagctgaa
      181 gcaggagaac cgtgaggccc gcaggaggat cgacctcaac ttggatatca gcccacagcg
      241 gcccaggccc accctgcaac tcccactggc caacgatggg ggcagccgct caccatcctc
      301 agagagctcc ccacagcacc ctacaccccc cacccggccc cgccacatgc tggggctccc
      361 atcaaccttg ttcacaccgc gcagtatgga gagcatcgag attgaccaga agctgcagga
      421 gatcatgaag cagacagggt acctgactat cgggggccag cgttatcagg cagaaatcaa
      481 tgacttggag aacttgggtg agatgggcag tggtacctgt ggtcaggtgt ggaagatgcg
      541 gttccggaag acaggccaca tcattgctgt taagcaaatg cggcgctctg ggaacaagga
      601 agagaataag cgcattttga tggacctgga tgtagtactc aagagccatg actgccctta
      661 catcgttcag tgctttggca ccttcatcac caacacagac gtctttattg ccatggagct
      721 catgggcaca tgtgcagaga agctgaagaa acgaatgcag ggccccattc cagagcgaat
      781 cctgggcaag atgactgtgg cgattgtgaa agcactgtac tatctgaagg agaagcatgg
      841 cgtcatccat cgcgatgtca actcat
//
